FlyBase Allele Report: Dmel\ab[k02807]

General Information

Symbol

Dmel\ab^k02807

Species

D. melanogaster

Name

FlyBase ID

FBal0064575

Feature type

allele

Associated gene

Dmel\ab

Associated Insertion(s)

P{lacW}ab^k02807

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}ab^k02807

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:11,221,528..11,221,528 [+]

(Tan et al., 2012, Li et al., 2004, Sugimura et al., 2004, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal posterior ventral multidendritic neuron vdap

(Li et al., 2004, Sugimura et al., 2004)

abdominal posterior ventral multidendritic neuron vdap (with ab^clu-1)

(Sugimura et al., 2004)

autophagic vacuole & larval fat body | somatic clone | cell autonomous

(Arsham and Neufeld, 2009)

larval dorsal multidendritic neuron ddaD

(Parrish et al., 2006)

larval dorsal multidendritic neuron ddaD | somatic clone

(Sugimura et al., 2004)

larval dorsal multidendritic neuron ddaE

(Parrish et al., 2006)

larval dorsal multidendritic neuron ddaE (with ab^clu-1)

(Sugimura et al., 2004)

larval dorsal multidendritic neuron ddaE | somatic clone

(Sugimura et al., 2004)

lobe system of adult mushroom body | somatic clone

(Kucherenko et al., 2012)

lysosome & larval fat body | somatic clone | cell autonomous

(Arsham and Neufeld, 2009)

mitochondrion & larval fat body | somatic clone | cell autonomous

(Arsham and Neufeld, 2009)

Detailed Description

Statement

Reference

ab^clu-1/ab^k02807 transheterozygotes die during early larval stages.

(Hattori et al., 2013)

Homozygous mushroom body neuron clones induced at the third instar larval stage migrate into α/β lobes (41%) or γ lobes (54%) (wild-type control clones induced at this stage are found in α'/β' lobes). Homozygous mushroom body neuron clones induced at the pupal stage form α/β lobes, as occurs for control clones.

(Kucherenko et al., 2012)

ab^k02807 clones in fat bodies from well-fed early third instar larvae accumulate lysosomes, as indicated by elevated LysoTracker staining, and show redistribution of the Atg8a product into autophagosomes. Mitochondria in ab^k02807 cells aggregate in large clumps.

(Arsham and Neufeld, 2009)

Targeting of dendrites to antennal lobe glomeruli occurs normally in antennal lobe projection neurons that are part of ab^k02807 homozygous somatic clones.

(Komiyama and Luo, 2007)

ab^k02807 mutants show a significant reduction in the number of class I neurons, an effect that is most pronounced in the dorsal cluster of PNS neurons.

(Parrish et al., 2006)

The vpda neurons of ab^k02807/+ larvae show an increased number of dendritic ends.

(Li et al., 2004)

ab^clu-1/ab^k02807 transheterozygotes hatch and grow normally until 30 hours AEL. The ddaE neurons of ab^clu-1/ab^k02807 larvae do not display such a comb-like design of dendritic arbors as wild-type ddaE neurons. Instead, mutant neurons produce supernumerary terminals that do not necessarily show oriented growth. About 20% of mutant ddaE cells do not extend lateral primary branches and in extreme cases, a single mutant ddaE extends two primary branches dorsally instead. Time-lapse recording of mutant ddaE shows that secondary branches appear with curved morphology and remain associated with fine higher-order branches that are oriented in dorsal and ventral directions. Ventral class I ventral posterior da (vpda) neurons also show dendritic pattern defects.

ddaD and ddaE ab^clu-1 mutant neuron clones in heterozygous mature larvae take on an abnormal shape abd produce branch terminals in excess. The ddaD neuron shows a statistically significant increase in the dendritic arbor area size while the area size of ddaE neurons is not significantly different. Class II, III and IV neuron clones do not show dendritic pattern defects.

(Sugimura et al., 2004)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

ab²⁶

(Li et al., 2004)

Rescued by

ab^k02807/ab^clu-1 is rescued by Scer\GAL4^pk-IG1-1/ab^UAS.L.cSa

(Sugimura et al., 2004)

ab^k02807/ab^clu-1 is rescued by ab^UAS.S.cSa/Scer\GAL4^pk-IG1-1

(Sugimura et al., 2004)

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

Bloomington

10520

y¹ w^*; P{lacW}ab^k02807 P{neoFRT}40A/CyO

Kyoto

102241

y¹ w^67c23 ; P{lacW}ab^k02807 / CyO

111153

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{lacW}ab^k02807 P{neoFRT}40A/CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

This allele was listed in the BDGP database as a lethal or sterile line during the period 1994-1999, but was discarded from the gene disruption project prior to the summary publication (FBrf0111489). Reasons for excluding lines from the collection described in FBrf0111489 include presence of more than one P insertion on the mutant chromosome, separation of lethality (or sterility) from the location of the insertion, and loss of lethality (or sterility) from the stock. Further information is available from http://www.fruitfly.org/bfd/ and from Dr. Spradling (spradling@mail1.ciwemb.edu).

Complements: l(2)k00224^k00224. Complements: cana^k07716. Complements: RfC38^k13807.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

ab^K02807

(Jang et al., 2009)

ab^k02807

(Yalgin et al., 2015, Hattori et al., 2013, Kucherenko et al., 2012, Tan et al., 2012, Arsham and Neufeld, 2009, Long et al., 2009, Komiyama and Luo, 2007, Kim et al., 2006, Parrish et al., 2006, Sugimura et al., 2004)

ab^l(2)K02807

(Li et al., 2004)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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