FlyBase Allele Report: Dmel\Rpn6[k00103]

General Information

Symbol

Dmel\Rpn6^k00103

Species

D. melanogaster

Name

FlyBase ID

FBal0064643

Feature type

allele

Associated gene

Dmel\Rpn6

Associated Insertion(s)

P{lacW}Rpn6^k00103

Carried in Construct

Also Known As

l(2)k00103

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Lier and Paululat, 2002)

Mutagen

P-element activity

(BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}Rpn6^k00103

Cytology

Description

P{lacW} insertion within the 5' UTR of Rpn6, 187bp upstream of the start codon.

(Lier and Paululat, 2002)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:14,752,221..14,752,221 [+]

(Lier and Paululat, 2002, Beaton, 2000.1.31, Spradling et al., 1999, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone

(Watts et al., 2003)

decreased cell number | somatic clone

(Watts et al., 2003)

lethal | recessive

(Beaton, 2000.1.31, Spradling et al., 1999, BDGP Project Members, 1994-1999)

lethal - all die before end of larval stage | recessive

(Lier and Paululat, 2002)

some die during larval stage | recessive

(Lier and Paululat, 2002)

Phenotype Manifest In

adult mushroom body | somatic clone

(Watts et al., 2003)

adult mushroom body gamma-lobe | somatic clone

(Watts et al., 2003)

neuroblast | somatic clone

(Watts et al., 2003)

Detailed Description

Statement

Reference

When homozygous clones are made no gross defects in axon guidance are seen in the mushroom body, however a block on axon pruning during metamorphosis is seen. As a consequence of this phenotype mutant γ neurons also maintain their dorsal projections. A reduction is also seen of cell numbers in neuroblast clones. Mutant clones show an age-dependant loss of cell number and axon projections.

(Watts et al., 2003)

Homozygous embryos develop and hatch normally, but during the first and second instar, the larvae become immobile and sluggish and die without any observable defects.

(Lier and Paululat, 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Rpn6[+]/Rpn6^k00103 is a suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Other

Statement

Reference

CSN4^k08018, Rpn6^k00103 has increased cell death | oogenesis phenotype

(Doronkin et al., 2003)

CSN5^L4032, Rpn6^k00103 has increased occurrence of cell division | oogenesis phenotype

(Doronkin et al., 2003)

Phenotype Manifest In

Suppressor of

Statement

Reference

Rpn6[+]/Rpn6^k00103 is a suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

Other

Statement

Reference

CSN4^k08018, Rpn6^k00103 has nurse cell phenotype

(Doronkin et al., 2003)

CSN4^k08018/CSN4[+], Rpn6^k00103 has nurse cell phenotype

(Doronkin et al., 2003)

CSN4^k08018/CSN4[+], Rpn6^k00103 has egg chamber phenotype

(Doronkin et al., 2003)

CSN5^L4032/CSN5[+], Rpn6^k00103 has nurse cell phenotype

(Doronkin et al., 2003)

CSN5^L4032/CSN5[+], Rpn6^k00103 has egg chamber phenotype

(Doronkin et al., 2003)

CSN4^k08018, Rpn6^k00103 has egg chamber phenotype

(Doronkin et al., 2003)

CSN4^k08018, Rpn6[+]/Rpn6^k00103 has nurse cell phenotype

(Doronkin et al., 2003)

CSN4^k08018, Rpn6[+]/Rpn6^k00103 has egg chamber phenotype

(Doronkin et al., 2003)

CSN5^L4032, Rpn6[+]/Rpn6^k00103 has nurse cell phenotype

(Doronkin et al., 2003)

CSN5^L4032, Rpn6[+]/Rpn6^k00103 has egg chamber phenotype

(Doronkin et al., 2003)

CSN5^L4032, Rpn6^k00103 has nurse cell phenotype

(Doronkin et al., 2003)

CSN5^L4032, Rpn6^k00103 has egg chamber phenotype

(Doronkin et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Unlike either single heterozygote, nurse cells in CSN4^k08018; Rpn6^k00103 or CSN5^L4032; Rpn6^k00103 transheterozygous females exhibit ectopic apoptosis, incorrect numbers of mitotic divisions and fusions of neighbouring egg chambers.

(Doronkin et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Rpn6^20F

(Lier and Paululat, 2002)

Rpn6^2F

(Lier and Paululat, 2002)

Rpn6^k14707

(BDGP Project Members, 1994-1999)