FlyBase Allele Report: Dmel\klu[UAS.Tag:MYC]

General Information

Symbol

Dmel\klu^UAS.Tag:MYC

Species

D. melanogaster

Name

FlyBase ID

FBal0064839

Feature type

allele

Associated gene

Dmel\klu

Associated Insertion(s)

Carried in Construct

P{UAS-klu.Myc}

Key Links

Transgenic product class

cDNA

(Klein and Campos-Ortega, 1997)

wild_type

(Klein and Campos-Ortega, 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Klein and Campos-Ortega, 1997)

wild_type

(Klein and Campos-Ortega, 1997)

Mutagen

in vitro construct

(Klein and Campos-Ortega, 1997)

Progenitor genotype

Carried in construct

P{UAS-klu.Myc}

Cytology

Description

A klu cDNA, tagged at the 3' end with Tag:MYC, is expressed under the control of UASt regulatory sequences.

(Klein and Campos-Ortega, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

klu

Tagged with

Tag:MYC

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^klu-G410

(Kaspar et al., 2008)

visible, with Scer\GAL4^sca-537.4

(Kaspar et al., 2008)

visible | somatic clone, with Scer\GAL4^Tub.PU

(Kaspar et al., 2008)

Phenotype Manifest In

chaeta | ectopic, with Scer\GAL4^klu-G410

(Kaspar et al., 2008)

chaeta | ectopic, with Scer\GAL4^sca-537.4

(Kaspar et al., 2008)

chaeta | ectopic | somatic clone, with Scer\GAL4^Tub.PU

(Kaspar et al., 2008)

chaeta | somatic clone | increased number, with Scer\GAL4^Tub.PU

(Kaspar et al., 2008)

external sensory organ precursor cell, with Scer\GAL4^klu-G410

(Kaspar et al., 2008)

external sensory organ precursor cell | increased number, with Scer\GAL4^klu-G410

(Kaspar et al., 2008)

macrochaeta | increased number, with Scer\GAL4^klu-G410

(Kaspar et al., 2008)

microchaeta | increased number, with Scer\GAL4^klu-G410

(Kaspar et al., 2008)

ommatidium | somatic clone, with Scer\GAL4^Tub.PU

(Kaspar et al., 2008)

trichome of the posterior wing margin | increased number, with Scer\GAL4^klu-G410

(Kaspar et al., 2008)

Detailed Description

Statement

Reference

Expression of klu^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^klu-G410 results in the formation of many additional macrochaetae and microchaetae throughout the adult body. In addition bristles develop at ectopic positions, such as on the mesopleura, head, halteres and third antennal segment. Supernumerary non-innervated bristles of the posterior wing margin are also seen.

Expression of klu^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^klu-G410 results in the formation of many supernumerary sensory organ precursor cells (SOPs) in the wing disc. In addition, the SOPs appear to develop earlier than wild type, as indicated by the premature presence of SOPs in the region of the prescutum at the late third larval instar stage.

Expression of klu^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^sca-537.4 results in the formation of ectopic bristles in the adult head.

Expression of klu^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^tub.PU in clones results in patches with higher bristle density in the notum and bristles at ectopic positions. When clones are located in the eye, ommatidia are replaced by bristles.

(Kaspar et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of klu^{Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^klu-G410 results in the recovery of only a very few bristles on the notum in Df(1)sc10-1 flies. There is an increase in the number of bristles formed on the legs in these flies compared to Df(1)sc10-1 flies.

(Kaspar et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^klu-G410/klu^UAS.Tag:MYC partially rescues klu^G410/klu^819lR5

(Klein and Campos-Ortega, 1997)

Scer\GAL4^klu-G410/klu^UAS.Tag:MYC partially rescues klu^G410/klu^212lR51C

(Klein and Campos-Ortega, 1997)

Comments

Activation of klu^{Scer\UAS.T:Hsap\MYC} by Scer\GAL4^klu-G410 in a klu^G410/klu^819lR5 or klu^G410/klu^212lR51C background leads to complete rescue of the bristle phenotype and partial rescue of the leg phenotype.

(Klein and Campos-Ortega, 1997)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

klu^{Scer\UAS.T:Hsap\MYC}

klu^UAS.T:Myc

klu^UAS.Tag:MYC

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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