FlyBase Allele Report: Dmel\mael[M391]

General Information

Symbol

Dmel\mael^M391

Species

D. melanogaster

Name

FlyBase ID

FBal0082460

Feature type

allele

Associated gene

Dmel\mael

Associated Insertion(s)

P{?lacW}mael^M391

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Findley et al., 2003)

Mutagen

P-element activity

(Findley et al., 2003)

Progenitor genotype

P{lacW}mael^11A4

(Findley et al., 2003)

Associated Insertion(s)

P{?lacW}mael^M391

Cytology

Description

Excision of the P{lacW} element, leaving a 17bp P-element residue, accompanied by a deletion of 1319bp of genomic DNA. The deletion removes nucleotides corresponding to 124-1270 of the mael mRNA.

(Findley et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | male

(Pek et al., 2009)

female sterile (with Df(3L)79E-F)

(Findley et al., 2003)

male sterile (with Df(3L)79E-F)

(Pek et al., 2009)

sterile (with Df(3L)79E-F)

(Sienski et al., 2012)

Phenotype Manifest In

cystoblast | increased number (with Df(3L)79E-F)

(Pek et al., 2012)

egg chamber

(Clegg et al., 1997)

female germline stem cell | increased number (with Df(3L)79E-F)

(Pek et al., 2012)

germarium region 3 (with Df(3L)79E-F)

(Pek et al., 2012)

germline cell

(Clegg et al., 1997)

karyosome

(Findley et al., 2003)

male germline cell (with Df(3L)79E-F)

(Pek et al., 2009)

male germline cyst (with Df(3L)79E-F)

(Pek et al., 2009)

male germline stem cell | ectopic (with Df(3L)79E-F)

(Pek et al., 2009)

ovary

(Findley et al., 2003)

spermatocyte ring canal (with Df(3L)79E-F)

(Pek et al., 2009)

Detailed Description

Statement

Reference

mael^M391/Df(3L)79E-F ovaries show a defect in germline stem cell differentiation, with approximately 70% of germaria containing more than six spectrosome-containing germline stem cells/cystoblasts, and fewer differentiating cysts than normal being seen. Approximately 80% of germaria have a 'two-oocyte phenotype' in region 3, indicating a delay in oocyte commitment (a single oocyte is already determined in approximately 70% of wild-type germaria by this stage).

(Pek et al., 2012)

A massive accumulation of early germ cells is seen in testes of 8-day old mael^M391/Df(3L)79E-F males. The accumulation of early germ cells becomes more severe as the flies age, and is accompanied by a drastic drop in fertility and the presence of fewer sperm bundles.

Mutant spermatogonia can undergo more than the normal four rounds of mitotic cell division to form cysts containing more than 16 cells, such as 32-cell cysts. In addition, cysts are seen harbouring abnormal numbers of cells instead of the normal 2, 4, 8 and 16, with many 2-cell cysts and single cells that mingle with larger cysts of abnormal cell number.

Single cells and cysts with spherical fusomes and remnants caused by the closure of ring canals are seen in mael^M391/Df(3L)79E-F testes.

Ectopic germline stem cell-like cells are seen both near and away from the hub and active mitosis of germline cells is seen away from the niche.

(Pek et al., 2009)

Mutant ovaries show some delay in restriction of synaptonemal complexes to a single cell. Mutant oocytes never form karyosomes and instead stage 5/6 oocytes show a nuclear morphology that is distinct from both stage 1 and the karyosome, the DNA forming variably distended loops and threads, often closely apposed to an invariably "deflated" nuclear envelope. This DNA morphology is maintained through at least stage 10B in mutant oocytes.

(Findley et al., 2003)

Hemizygous oocytes exhibit rapid depletion of germ cells after eclosion and egg chambers have A/P and D/V axis defects.

(Clegg et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

mael^M391/Df(3L)79E-F has male germline cell phenotype, suppressible by bam^hs.PO

(Pek et al., 2009)

mael^M391/Df(3L)79E-F has male germline cell phenotype, suppressible by Dcr-1^Q1147X/Dcr-1[+]

(Pek et al., 2009)

mael^M391/Df(3L)79E-F has male germline cell phenotype, suppressible by loqs[+]/loqs^f00791

(Pek et al., 2009)

mael^M391/Df(3L)79E-F has male germline cell phenotype, suppressible by Df(2R)mir-7-Δ1/mir-7[+]

(Pek et al., 2009)

NOT suppressed by

Statement

Reference

mael^M391/Df(3L)79E-F has male germline cell phenotype, non-suppressible by HnRNP-K^k08305/bl[+]

(Pek et al., 2009)

mael^M391/Df(3L)79E-F has male germline cell phenotype, non-suppressible by ban[+]/mir-ban^unspecified

(Pek et al., 2009)

Additional Comments

Genetic Interactions

Statement

Reference

The differentiation defects seen in the testes of mael^M391/Df(3L)79E-F males are rescued by expression of bam^hs.PO.

The differentiation defects seen in the testes of mael^M391/Df(3L)79E-F males are partially rescued by Dcr-1^Q1147X/+, loqs^f00791/+ or Df(2R)mir-7-Δ1/+.

The differentiation defects seen in the testes of mael^M391/Df(3L)79E-F males are not rescued by bl^k08305/+ or by ban^unspecified/+.

(Pek et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mael^M391/Df(3L)79E-F is rescued by Scer\GAL4^nanos.PG/mael^{UASp.Tag:FLAG}

(Pek et al., 2012)

mael^M391/Df(3L)79E-F is rescued by mael^{Tag:FLAG,EGFP}

(Sienski et al., 2012)

mael^M391/Df(3L)79E-F is rescued by mael^+t4.5

(Findley et al., 2003)

Partially rescued by

mael^M391/Df(3L)79E-F is partially rescued by Scer\GAL4^nanos.PG/mael^{S138A.UASp.Tag:FLAG}

(Pek et al., 2012)

mael^M391/Df(3L)79E-F is partially rescued by Scer\GAL4^nanos.PG/mael^{S138D.UASp.Tag:FLAG}

(Pek et al., 2012)

mael^M391/Df(3L)79E-F is partially rescued by mael^{ΔHMG.Tag:FLAG,EGFP}

(Sienski et al., 2012)

Not rescued by

mael^M391/Df(3L)79E-F is not rescued by mael^H291A.EGFP

(Sienski et al., 2012)

mael^M391/Df(3L)79E-F is not rescued by mael^E131A.EGFP

(Sienski et al., 2012)

Comments

Expression of either mael^{S138A.Scer\UAS.P\T.T:Zzzz\FLAG} or mael^{S138D.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^nos.PG rescues the differentiation defect seen in mael^M391/Df(3L)79E-F ovaries, such that the number of germline stem cells and cystoblasts returns to normal and robust differentiation is seen.

Expression of mael^{Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^nos.PG rescues the defect in oocyte commitment (high frequency of 'two-oocyte phenotype' in region 3 of the germarium) that is seen in mael^M391/Df(3L)79E-F germaria.

Expression of mael^{S138A.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^nos.PG does not rescue the defect in oocyte commitment (high frequency of 'two-oocyte phenotype' in region 3 of the germarium) that is seen in mael^M391/Df(3L)79E-F germaria.

Expression of mael^{S138D.Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^nos.PG partially rescues the defect in oocyte commitment (high frequency of 'two-oocyte phenotype' in region 3 of the germarium) that is seen in mael^M391/Df(3L)79E-F germaria.

(Pek et al., 2012)

The accumulation of early germ cells which is seen in the testes of mael^M391/Df(3L)79E-F males is rescued by expression of mael^{Scer\UAS.P\T.T:Zzzz\FLAG} under the control of Scer\GAL4^nos.PG.

(Pek et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mael^M391

(Onishi et al., 2020, Minakhina et al., 2014, Patil et al., 2014, Anand and Kai, 2012, Sienski et al., 2012, Nagao et al., 2010, Patil and Kai, 2010, Pek et al., 2009, Lim and Kai, 2007, Lim and Kai, 2007)

maelM391

(Pek et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (13)

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