FlyBase Allele Report: Dmel\brm[K804R.Tag:HA]

General Information

Symbol

Dmel\brm^K804R.Tag:HA

Species

D. melanogaster

Name

FlyBase ID

FBal0086706

Feature type

allele

Associated gene

Dmel\brm

Associated Insertion(s)

Carried in Construct

P{brm^K804R.Tag:HA}

Also Known As

brm^K804R

Key Links

Transgenic product class

dominant_negative_variant

(Elfring et al., 1998)

inframe_deletion

(Elfring et al., 1998)

missense_variant

(Elfring et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Elfring et al., 1998)

inframe_deletion

(Elfring et al., 1998)

missense_variant

(Elfring et al., 1998)

Mutagen

in vitro construct

(Elfring et al., 1998, Moseley et al., 1998, Papoulas et al., 1998)

Progenitor genotype

brm^Tag:HA

(Elfring et al., 1998)

Carried in construct

P{brm^K804R.Tag:HA}

Cytology

Description

Amino acid replacement: K804R. K804R falls in the ATP-binding site.

(Elfring et al., 1998)

Amino acid replacement: K804R.

(Moseley et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

brm

Encoded product / tool

brm

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | recessive

(Elfring et al., 1998)

Phenotype Manifest In

capitellum

(Elfring et al., 1998)

capitellum & macrochaeta

(Elfring et al., 1998)

haltere

(Elfring et al., 1998)

macrochaeta | ectopic

(Elfring et al., 1998)

Detailed Description

Statement

Reference

Flies carrying one or two copies of brm^{K804R.T:Ivir\HA1} are viable. Flies carrying two copies of brm^{K804R.T:Ivir\HA1} in a wild-type background frequently show partial transformation of haltere to wing, as shown by an increase in haltere size and the appearance of ectopic bristles on the capitellum. This phenotype is also seen in Df(3L)th102/+ animals carrying two copies of brm^{K804R.T:Ivir\HA1}. brm²/Df(3L)th102 animals carrying two copies of brm^{K804R.T:Ivir\HA1} do not survive to adulthood.

(Elfring et al., 1998)

Female brm deficiency heterozygotes carrying an X-linked copy of brm^{K804R.T:Ivir\HA1} survive to adulthood, but males of this genotype are not viable.

(Papoulas et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

brm^K804R.Tag:HA/brm[+] is a suppressor of visible | dominant phenotype of Egfr^E3

(Herr et al., 2010)

Other

Statement

Reference

Hsc70-4⁰³⁵⁵⁰, brm^K804R.Tag:HA has viable | male phenotype

(Mollaaghababa et al., 2001)

Hsc70-4^54.1, brm^K804R.Tag:HA has viable | male phenotype

(Mollaaghababa et al., 2001)

Hsc70-4^II-R35-R7, brm^K804R.Tag:HA has viable | male phenotype

(Mollaaghababa et al., 2001)

Phenotype Manifest In

Suppressor of

Statement

Reference

brm^K804R.Tag:HA/brm[+] is a suppressor of eye phenotype of Egfr^E3

(Herr et al., 2010)

brm^K804R.Tag:HA is a suppressor of eye phenotype of CycE^JP

(Brumby et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

The small rough eye phenotype of Egfr^E3/+ is suppressed by expression of the brm^{K804R.T:Ivir\HA1} genomic construct.

(Herr et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

brm^K804R.Tag:HA fails to rescue Df(3L)th102/brm²

(Elfring et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

An engineered dominant-negative mutation.

(Moseley et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

brm^DN

(Herr et al., 2010)

brm^K804R.T:HA1

brm^{K804R.T:Ivir\HA1}

brm^K804R.Tag:HA

brm^K804R

(Armstrong et al., 2005, Marenda et al., 2004, Zraly et al., 2004, Elfring et al., 1998, Moseley et al., 1998, Papoulas et al., 1998)

brmDN

(Herr et al., 2010)

Name Synonyms

Secondary FlyBase IDs

FBal0086704

References (9)

Report Sections

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