FB2026_02 , released June 18, 2026
Allele: Dmel\brmK804R.Tag:HA,Tag:polyHis
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General Information
Symbol
Dmel\brmK804R.Tag:HA,Tag:polyHis
Species
D. melanogaster
Name
FlyBase ID
FBal0086706
Feature type
allele
Associated gene
Dmel\brm
Associated Insertion(s)
Carried in Construct
P{brmK804R.Tag:HA}
Also Known As
brmK804R
Key Links
Transgenic product class
dominant_negative_variant
(Elfring et al., 1998)
inframe_deletion
(Elfring et al., 1998)
missense_variant
(Elfring et al., 1998)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Elfring et al., 1998)
inframe_deletion
(Elfring et al., 1998)
missense_variant
(Elfring et al., 1998)
Mutagen
in vitro construct
(Elfring et al., 1998, Moseley et al., 1998, Papoulas et al., 1998)
Progenitor genotype
brmTag:HA,Tag:polyHis
(Elfring et al., 1998)
Carried in construct
P{brmK804R.Tag:HA}
Cytology
Description

14.4kb BamHI-EcoRI genomic fragment that encompasses brm. The C-terminal two residues of brm have been replaced by sequence encoding the Tag:HA epitope followed by six His residues (details from FBrf0083116). A K804R mutation has been introduced into the brm coding sequence; this mutation is within the ATP-binding site and results in a dominant-negative protein.

(Elfring et al., 1998)
Allele components
Component
Use(s)
Regulatory region(s)
brm
Encoded product / tool
brm
Tagged with
Tag:HA
Tag:polyHis
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies carrying one or two copies of brmK804R.T:Ivir\HA1 are viable. Flies carrying two copies of brmK804R.T:Ivir\HA1 in a wild-type background frequently show partial transformation of haltere to wing, as shown by an increase in haltere size and the appearance of ectopic bristles on the capitellum. This phenotype is also seen in Df(3L)th102/+ animals carrying two copies of brmK804R.T:Ivir\HA1. brm2/Df(3L)th102 animals carrying two copies of brmK804R.T:Ivir\HA1 do not survive to adulthood.

      (Elfring et al., 1998)

      Female brm deficiency heterozygotes carrying an X-linked copy of brmK804R.T:Ivir\HA1 survive to adulthood, but males of this genotype are not viable.

      (Papoulas et al., 1998)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      brmK804R.Tag:HA,Tag:polyHis/brm[+] is a suppressor of eye phenotype of EgfrE3

      (Herr et al., 2010)

      brmK804R.Tag:HA,Tag:polyHis is a suppressor of eye phenotype of CycEJP

      (Brumby et al., 2002)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The small rough eye phenotype of EgfrE3/+ is suppressed by expression of the brmK804R.T:Ivir\HA1 genomic construct.

      (Herr et al., 2010)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Fails to rescue

      brmK804R.Tag:HA,Tag:polyHis fails to rescue Df(3L)th102/brm2

      (Elfring et al., 1998)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (7)
      Reported As
      Symbol Synonym
      brmDN
      (Herr et al., 2010)
      brmK804R.T:HA1
      brmK804R.T:Ivir\HA1
      brmK804R.Tag:HA,Tag:polyHis
      brmK804R.Tag:HA
      brmK804R
      (Armstrong et al., 2005, Marenda et al., 2004, Zraly et al., 2004, Elfring et al., 1998, Moseley et al., 1998, Papoulas et al., 1998)
      brmDN
      (Herr et al., 2010)
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0086704
      References (9)