FlyBase Allele Report: Dmel\Met[27]

General Information

Symbol

Dmel\Met²⁷

Species

D. melanogaster

Name

FlyBase ID

FBal0089546

Feature type

allele

Associated gene

Dmel\Met

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Wilson et al., 2006, Wilson and Ashok, 1998, Wilson et al., 1998)

Mutagen

X ray

(Ashok et al., 1998)

60Co gamma ray

(Wilson and Ashok, 1998)

gamma ray

(Wilson et al., 2006)

Progenitor genotype

Cytology

Description

Probably a chromosomal break in the transcriptional regulatory region.

(Wilson and Ashok, 1998)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

Met²⁷ flies tolerate dietary methoprene better than Met[+] controls.

(Jindra et al., 2015)

Mutant larvae are resistant to the juvenile hormone agonist pyriproxyfen compared to wild type.

Mutant females show reduced fecundity: they begin ovipositing at 6.0 days after eclosion (in contrast to 3.0 days for wild-type females) and after 30 days, the total number of eggs laid by a single mutant female is only 37% that of wild type. The hatching rate of the mutant eggs is only 78%.

(Abdou et al., 2011)

Mutant males treated with methoprene during larval development have normal appearing genitalia (wild-type control males show a failure of rotation of the genital disc under these conditions).

(Baumann et al., 2010)

Mutant prepupae show reduced proliferation in both the outer and the inner proliferation zones of the optic lobe at an early time compared to controls. In addition, they show precocious separation of the axons of photoreceptors R7 and R8 in the medulla which is evident by 6 hours after puparium formation. The mutants show a two-hour advancement in the ingrowth of lamina neurons into the medulla compared to controls.

The gross anatomy of the lamina and medulla appears normal in mutant adults and there is no mistargeting of photoreceptor axons to inappropriate layers. However, the lobula shows gross abnormalities with irregular lobes that project towards, or sometimes penetrate, the medulla neuropil. These projections severely disrupt the layering of the lobula and the lobula plate, such that neurons that normally have their dendrites confined to a single layer now project in a disorganised fashion throughout the lobula. The beginnings of these irregular projections are evident at 12 hours after puparium formation.

(Riddiford et al., 2010)

Rst(1)JH²⁷ flies show good survival, with <15% mortality during pupal development. Rst(1)JH²⁷ females show only ~20% of the level of oogenesis seen in wild-type females.

(Wilson et al., 2006)

Homozygotes show increased resistance to methoprene compared to controls.

Homozygous females have a reduced rate of oviposition compared to controls.

66% of mutant flies have a small number of defective ommatidia in the posterior quarter of the eye.

(Wilson et al., 2006)

Under normal conditions, 1 day old mutant females show a significantly lower juvenile hormone (JH)-hydrolysing activity compared to wild-type females. Mutant and wild-type females exposed to 38^oC show a significant decrease in JH-hydrolysing activity compared to control females at 25^oC. 1 day old mutant females have lower levels of stress reactivity (calculated as the percent decrease in JH-hydrolysing activity at 38^oC relative to normal conditions) than control females. Under normal conditions, the level of JH degradation in 5 day old mutant females is the same as in control females. After exposure to 38^oC, mature mutant female show no changes in the level of JH metabolism, in contrast to control females which show a significant decrease in JH-hydrolysing activity.

(Gruntenko et al., 2000)

Mutant females have significantly lower fertility than control flies at 25^oC. At 38^oC, control females show a much larger decrease in fertility compared to 25^oC than mutant females.

(Gruntenko et al., 2000)

Homozygous larvae show resistance to both the toxic and morphogenetic effects of methoprene compared to wild-type larvae. Heterozygous larvae show partial resistance to the morphogenetic effects of methoprene, but otherwise appear wild-type. Homozygous and hemizygous females show reduced oviposition; the onset of oviposition is delayed and the rate of oviposition is lower than wild-type. Homozygous females have fewer vitellogenic oocytes than wild-type females.

(Wilson and Ashok, 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Met²⁷, gce^2.5k has some die during P-stage phenotype, suppressible | partially by Met^{+tCH321-28O05}

(Baumann et al., 2017)

Met²⁷, gce^2.5k has some die during P-stage phenotype, suppressible by gce^{+tCH321-09E09}

(Baumann et al., 2017)

Met²⁷, gce^2.5k has lethal phenotype, suppressible by Scer\GAL4^arm.PU/Met^UAS.cJa

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, suppressible by Scer\GAL4^Tub.PU/Met^UAS.cJa

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, suppressible by gce^UAS.cJa/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, suppressible by gce^UAS.cJa/Scer\GAL4^Tub.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, suppressible by gce^UAS.Tag:FLAG/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, suppressible by gce^UAS.Tag:FLAG/Scer\GAL4^Tub.PU

(Jindra et al., 2015)

Met²⁷ has chemical resistant phenotype, suppressible by gce^UAS.cJa/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷ has chemical resistant phenotype, suppressible by gce^UAS.cBa/Scer\GAL4^Act5C.PI

(Baumann et al., 2010)

Met²⁷ has chemical resistant phenotype, suppressible by gce^UAS.cBa/Scer\GAL4^Tub.PU

(Baumann et al., 2010)

Met²⁷ has chemical resistant phenotype, suppressible by gce^UAS.cBa/Scer\GAL4^Lsp2.PH

(Baumann et al., 2010)

NOT suppressed by

Statement

Reference

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^T272Y.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^T272Y.UAS/Scer\GAL4^Tub.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^{T272Y.UAS.Tag:FLAG}/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^{T272Y.UAS.Tag:FLAG}/Scer\GAL4^Tub.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^{V315F.UAS.Tag:FLAG}/Scer\GAL4^Tub.PU

(Jindra et al., 2015)

Met²⁷ has chemical resistant phenotype, non-suppressible by gce^T272Y.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷ has chemical resistant phenotype, non-suppressible by gce^V315F.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷ has chemical resistant phenotype, non-suppressible by gce^C366M.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^V315F.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by Scer\GAL4^Tub.PU/gce^V315F.UAS

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^C366M.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by gce^C366M.UAS/Scer\GAL4^Tub.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by Met^V449F.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by Scer\GAL4^Tub.PU/Met^V449F.UAS

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by Met^C500M.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype, non-suppressible by Scer\GAL4^Tub.PU/Met^C500M.UAS

(Jindra et al., 2015)

Enhancer of

Statement

Reference

Met²⁷ is an enhancer of lethal phenotype of Scer\GAL4^Tub.PU, gce^RNAi.UAS.cBa

(Baumann et al., 2010)

Met²⁷/Met[+] is an enhancer of lethal phenotype of br^rbp-1

(Wilson et al., 2006)

Suppressor of

Statement

Reference

Met¹, gce^2.5k, Met²⁷, gce^MI02742 is a suppressor | partially of abnormal cell migration | parental effect | embryonic stage phenotype of Hmgcr^UAS.cvDa, Scer\GAL4^48Y

(Barton et al., 2024)

Other

Statement

Reference

Met¹/Met²⁷, gce^2.5k/gce^MI02742 has partially lethal - majority die phenotype

(Barton et al., 2024)

Met¹/Met²⁷, gce^2.5k/gce^MI02742 has decreased fecundity phenotype

(Barton et al., 2024)

Met¹/Met²⁷, gce^2.5k/gce^MI02742 has abnormal cell migration | maternal effect | embryonic stage phenotype

(Barton et al., 2024)

Met²⁷, gce^2.5k has some die during P-stage phenotype

(Baumann et al., 2017)

Met²⁷, Scer\GAL4^arm.PU, gce^UAS.cJa has chemical sensitive phenotype

(Jindra et al., 2015)

Met²⁷, gce^2.5k has lethal phenotype

(Jindra et al., 2015)

Met²⁷, gce^2.5k has increased cell death phenotype

(Abdou et al., 2011)

Met²⁷, gce^2.5k has decreased body size | larval stage phenotype

(Abdou et al., 2011)

Met²⁷, gce^2.5k has abnormal developmental rate | larval stage phenotype

(Abdou et al., 2011)

Met²⁷, gce^2.5k has lethal | P-stage phenotype

(Abdou et al., 2011)

Met²⁷, gce^2.5k has decreased body size | P-stage phenotype

(Abdou et al., 2011)

Met²⁷, Scer\GAL4^Tub.PU, gce^RNAi.UAS.cBa has lethal | pupal stage phenotype

(Baumann et al., 2010)

Met²⁷, Scer\GAL4^Act5C.PI, gce^RNAi.UAS.cBa has lethal | pupal stage phenotype

(Baumann et al., 2010)

Met²⁷, br^rbp-1/br⁵ has lethal phenotype

(Wilson et al., 2006)

Met²⁷, br^rbp-2/br⁵ has lethal phenotype

(Wilson et al., 2006)

Met²⁷, br⁵/br^2Bc-1 has partially lethal phenotype

(Wilson et al., 2006)

Met²⁷, br^rbp-2 has lethal - all die during P-stage phenotype

(Wilson et al., 2006)

Met²⁷, br⁵/br¹ has lethal - all die during P-stage phenotype

(Wilson et al., 2006)

Met²⁷, br^rbp-1/br^2Bc-1 has lethal phenotype

(Wilson et al., 2006)

Met²⁷, br^rbp-2/br^rbp-1 has lethal - all die during P-stage phenotype

(Wilson et al., 2006)

Met²⁷, br¹/br^2Bc-1 has viable phenotype

(Wilson et al., 2006)

Met²⁷, br^rbp-2/br^2Bc-1 has viable phenotype

(Wilson et al., 2006)

Met²⁷, br¹ has lethal phenotype

(Wilson et al., 2006)

Met²⁷, br¹ has lethal - all die during P-stage phenotype

(Wilson et al., 2006)

Met²⁷/Met[+], br^rbp-1 has lethal - all die during P-stage phenotype

(Wilson et al., 2006)

Phenotype Manifest In

Enhanced by

Statement

Reference

Met²⁷ has ovary phenotype, enhanceable by br^rbp-2/br^rbp-2

(Wilson et al., 2006)

Met²⁷ has ovary phenotype, enhanceable by br¹/br¹

(Wilson et al., 2006)

Enhancer of

Statement

Reference

Met²⁷/Met²⁷ is an enhancer of ovary phenotype of br¹

(Wilson et al., 2006)

Met²⁷/Met²⁷ is an enhancer of ovary phenotype of br^rbp-2

(Wilson et al., 2006)

Suppressor of

Statement

Reference

Met¹, gce^2.5k, Met²⁷, gce^MI02742 is a suppressor | partially of primordial germ cell | parental effect | embryonic stage phenotype of Hmgcr^UAS.cvDa, Scer\GAL4^48Y

(Barton et al., 2024)

Other

Statement

Reference

Met¹/Met²⁷, gce^2.5k/gce^MI02742 has primordial germ cell | maternal effect | embryonic stage phenotype

(Barton et al., 2024)

Met²⁷, gce^2.5k has fat body phenotype

(Abdou et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Met²⁷, gce^2.5k double mutant females (crossed to control males) show 25.9% pupal lethality; this lethality is fully rescued upon expression of gce^{+tCH321-09E09}, but only partially rescued upon expression of Met^{+tCH321-28O05}.

(Baumann et al., 2017)

Met²⁷ flies expressing gce^Scer\UAS.cJa under the control of Scer\GAL4^arm.PU are sensitive to dietary methoprene, showing a greater sensitivity than wild-type controls.

Expression of either gce^{T272Y.Scer\UAS}, gce^{V315F.Scer\UAS} or gce^{C366M.Scer\UAS} under the control of Scer\GAL4^arm.PU in Met²⁷ flies does not restore sensitivity to dietary methoprene.

(Jindra et al., 2015)

Onset of metamorphosis (larval wandering stage) is delayed approximately 12 hours compared to wild type in Met²⁷ gce^2.5k double mutant larvae. Double mutant larvae and early pupae have a reduced body size compared to controls. The double mutants fail to undergo head eversion and die approximately 24 hours after pupariation. Fat body cells in the double mutants undergo precocious and enhanced programmed cell death compared to wild-type fat body cells.

(Abdou et al., 2011)

Expression of gce^Scer\UAS.cBa under the control of either Scer\GAL4^Act5C.PI, Scer\GAL4^tub.PU or Scer\GAL4^Lsp2.PH reduces the increased resistance to methoprene which is seen in Met²⁷ animals.

Met²⁷ males expressing gce^Scer\UAS.cBa under the control of Scer\GAL4^tub.PU and treated with methoprene during larval development show abnormal genital rotation (a response similar to wild-type males raised under these conditions).

(Baumann et al., 2010)

While 86% of br^rbp-2/br^rbp-2, Rst(1)JH²⁷/+ flies survive to adulthood, <2% of br^rbp-2/br^rbp-2, Rst(1)JH²⁷/Rst(1)JH²⁷ flies survive this far, with most dying during the pharate adult stage. br^rbp-1/br^rbp-1, Rst(1)JH²⁷/+ double mutants die at an earlier stage (prepupal/early pupal) than br^rbp-1/br^rbp-1 mutants. Expression of the Rst(1)JH^+tSt-H.hs transgene can rescue the lethality of br^rbp-2/br^rbp-2, Rst(1)JH²⁷/Rst(1)JH²⁷ or br^rbp-2/br^rbp-1, Rst(1)JH²⁷/Rst(1)JH²⁷ flies. br¹/br¹, Rst(1)JH²⁷/Rst(1)JH²⁷ mutants are lethal in both pupal and especially pharate adult stages, but small numbers of escaper adults eclose. When these mutants express the Rst(1)JH^+tSt-H.hs transgene, they survive to adulthood. Like br⁵ single mutants, br⁵, Rst(1)JH²⁷ double mutants are lethal in prepupal/early pupal development. 54% of br⁵/br¹, Rst(1)JH²⁷/+ mutants survive to adulthood but br⁵/br¹, Rst(1)JH²⁷/Rst(1)JH²⁷ mutants are lethal in prepupal/early pupal development. br¹/br¹, Rst(1)JH²⁷/Rst(1)JH²⁷ and br^rbp-2/br^rbp-2, Rst(1)JH²⁷/Rst(1)JH²⁷ female escapers show strong reductions in oogenesis.

(Wilson et al., 2006)

The expressivity of the defective eye phenotype seen in Met²⁷ flies is increased by br^rbp-2 but not by br¹.

(Wilson et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Met³

(Wilson and Ashok, 1998)

Rescued by

Met²⁷ is rescued by Scer\GAL4^arm.PU/Met^UAS.cJa

(Jindra et al., 2015)

Not rescued by

Met²⁷ is not rescued by Met^V449F.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Met²⁷ is not rescued by Met^C500M.UAS/Scer\GAL4^arm.PU

(Jindra et al., 2015)

Comments

Expression of Met^Scer\UAS.cJa under the control of Scer\GAL4^arm.PU in Met²⁷ flies restores sensitivity to dietary methoprene.

(Jindra et al., 2015)

The lethality of Met²⁷ gce^2.5k double mutants is fully rescued by a single copy of Met^+t5.7 or by expression of gce^Scer\UAS.cBa under the control of Scer\GAL4^arm.PS.

(Abdou et al., 2011)

The defective eye phenotype seen in Met²⁷ flies is rescued by Met^+tSt-H.hs.

(Wilson et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

90979

w^* v^* Met²⁷

Notes on Origin

Discoverer

Comments

Transformation of Rst(1)JH²⁷ mutants with a Rst(1)JH⁺ cDNA restores methoprene susceptibility.

(Wilson et al., 1998)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Met27

(Gruntenko et al., 1999)

Rst(1)JH²⁷

Rst(1)JH^Met-27

met²⁷

(Arquier et al., 2021)

Name Synonyms

Secondary FlyBase IDs

FBal0086770

References (29)

Report Sections

Open Close