FlyBase Allele Report: Dmel\gbb[4]

General Information

Symbol

Dmel\gbb⁴

Species

D. melanogaster

Name

FlyBase ID

FBal0092972

Feature type

allele

Associated gene

Dmel\gbb

Associated Insertion(s)

Carried in Construct

Also Known As

gbb-60A⁴

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Bangi and Wharton, 2006, Ray and Wharton, 2001, Wharton et al., 1999, Khalsa et al., 1998)

Mutagen

Progenitor genotype

Cytology

Description

Nucleotide substitution: C1607T.

(Wharton et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:23,852,956..23,852,956 [+]

Nucleotide change:

C23852956T

Reported nucleotide change:

C1607T

Amino acid change:

A401V | gbb-PA; A401V | gbb-PB

Reported amino acid change:

A380V

Comment:

The reported site of gbb⁴ in FBrf0109147 is base 1607 and amino acid 380 (Ala). This is not consistent because base 1607 actually corresponds to amino acid 401 which is also an Ala. The latter is the presumed site of the mutation since the codon (GCG) at that site matches the codon sequence reported in the paper while the codon corresponding to aa 380 is GCC.

(Wharton et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate (with gbb¹)

(Ralston and Blair, 2005)

abnormal neuroanatomy (with gbb¹)

(McCabe et al., 2003)

abnormal neurophysiology (with gbb¹)

(McCabe et al., 2003)

lethal, with gbb^{R126A.R325A.K334N.Tag:FLAG}

(Fritsch et al., 2012)

lethal (with gbb¹)

(Ray and Wharton, 2001)

lethal (with gbb^D20)

(Fritsch et al., 2010)

lethal | recessive

(Haerry et al., 1998)

partially lethal - majority live

(Christoforou et al., 2008)

viable

(Wharton et al., 1999, Khalsa et al., 1998)

viable (with gbb³)

(Bangi and Wharton, 2006)

visible

(Wharton et al., 1999)

visible (with gbb¹)

(Fritsch et al., 2010, Bangi and Wharton, 2006, Ray and Wharton, 2001)

visible (with gbb³)

(Ray and Wharton, 2001)

visible (with gbb^5I)

(Bangi and Wharton, 2006)

visible | adult stage (with gbb^5I)

(Tauscher et al., 2016)

visible | recessive

(Yu et al., 2000, Khalsa et al., 1998)

Phenotype Manifest In

anterior crossvein

(Khalsa et al., 1998)

bouton (with gbb¹)

(McCabe et al., 2003)

crossvein (with gbb¹)

(Bangi and Wharton, 2006)

discal wing cell

(Khalsa et al., 1998)

dorsocentral bristle

(Wharton et al., 1999)

eye

(Wharton et al., 1999, Khalsa et al., 1998)

female germline stem cell | heat sensitive (with gbb^D4)

(Song et al., 2004)

female germline stem cell | heat sensitive (with gbb^D20)

(Song et al., 2004)

hemocyte | pupal stage

(Christoforou et al., 2008)

leg

(Khalsa et al., 1998)

macrochaeta | ectopic

(Wharton et al., 1999)

male germline stem cell (with gbb¹)

(Shivdasani and Ingham, 2003)

male germline stem cell | heat sensitive (with gbb^D4)

(Kawase et al., 2004)

male germline stem cell | heat sensitive (with gbb^D20)

(Kawase et al., 2004)

mesothoracic bristle

(Khalsa et al., 1998)

metathoracic bristle

(Khalsa et al., 1998)

ommatidium

(Wharton et al., 1999)

posterior crossvein

(Wharton et al., 1999, Haerry et al., 1998, Khalsa et al., 1998)

posterior crossvein (with gbb¹)

(McCabe et al., 2003, Ray and Wharton, 2001)

posterior crossvein (with gbb³)

(Ray and Wharton, 2001)

posterior crossvein (with gbb^5I)

(Tauscher et al., 2016)

posterior crossvein (with gbb^D20)

(Fritsch et al., 2010)

postsynaptic membrane (with gbb¹)

(McCabe et al., 2003)

scutellar bristle

(Wharton et al., 1999)

scutellar bristle | ectopic

(Wharton et al., 1999)

second basal wing cell

(Khalsa et al., 1998)

second posterior wing cell

(Khalsa et al., 1998)

spermatocyte (with gbb¹)

(Shivdasani and Ingham, 2003)

submarginal wing cell

(Khalsa et al., 1998)

synaptic vesicle (with gbb¹)

(McCabe et al., 2003)

testis

(Shivdasani and Ingham, 2003)

testis (with gbb¹)

(Shivdasani and Ingham, 2003)

third posterior wing cell

(Khalsa et al., 1998)

vibrissal bristle

(Khalsa et al., 1998)

wing

(Wharton et al., 1999, Khalsa et al., 1998)

wing (with gbb¹)

(Ray and Wharton, 2001)

wing (with gbb³)

(Ray and Wharton, 2001)

wing cell

(Khalsa et al., 1998)

wing margin bristle | ectopic

(Khalsa et al., 1998)

wing sensillum

(Khalsa et al., 1998)

wing vein

(Yu et al., 2000, Wharton et al., 1999, Khalsa et al., 1998)

wing vein (with gbb¹)

(Fritsch et al., 2010)

wing vein | ectopic

(Wharton et al., 1999, Khalsa et al., 1998)

wing vein L2

(Wharton et al., 1999, Khalsa et al., 1998)

wing vein L4

(Wharton et al., 1999, Haerry et al., 1998)

wing vein L4 (with gbb^5I)

(Bangi and Wharton, 2006)

wing vein L4 | distal

(Khalsa et al., 1998)

wing vein L4 | distal (with gbb¹)

(Ray and Wharton, 2001)

wing vein L4 | distal (with gbb³)

(Ray and Wharton, 2001)

wing vein L5

(Wharton et al., 1999, Haerry et al., 1998, Khalsa et al., 1998)

wing vein L5 (with gbb¹)

(Bangi and Wharton, 2006, McCabe et al., 2003)

wing vein L5 (with gbb³)

(Ray and Wharton, 2001)

wing vein L5 (with gbb^5I)

(Bangi and Wharton, 2006)

wing vein L5 (with gbb^D20)

(Fritsch et al., 2010)

wing vein L5 | distal (with gbb¹)

(Ray and Wharton, 2001)

Detailed Description

Statement

Reference

gbb⁴ homozygotes show complete lethality in the presence of one copy of gbb^{R126A.R325A.K334N.T:Zzzz\FLAG}.

(Fritsch et al., 2012)

Rare gbb⁴/gbb^D20 adult escapers have small wings that lack the posterior crossvein and the distal quarter of vein L5.

(Fritsch et al., 2010)

Hemocytes do not localise to the posterior crossvein region in pupal wings homozygous for gbb⁴, in contrast to wild-type.

(Christoforou et al., 2008)

The wings of gbb¹/gbb⁴ flies show a loss of the L4-L5 intervein and a truncation of wing vein L5.

gbb⁴/gbb^5I wings have a small gap in both the L4 and L5 wing veins.

(Bangi and Wharton, 2006)

In gbb⁴/gbb^D4 males raised to adulthood at 18^oC and then shifted to 22^oC for 7 days, testes contain an average 1.3 germline stem cells compared to an average of 9.1 in wild-type. Germline stem cells are eliminated completely after a week at 25^oC. In gbb⁴/gbb^D20 males raised to adulthood at 18^oC and then shifted to 22^oC for 7 days, testes contain pn average 1.8 germline stem cells compared to an average of 9.1 in wild-type. Germline stem cells are eliminated completely after a week at 25^oC.

(Kawase et al., 2004)

In gbb⁴/gbb^D4 females raised to adulthood at 18^oC and then shifted to 29^oC for 7 days, 88% of germaria lack germ cells. Remaining germaria mostly contain only one germ cell. In identically treated gbb⁴/gbb^D20 females, 60% of germaria lack germ cells, and the remaining germaria mostly contain only one germ cell.

(Song et al., 2004)

gbb¹/gbb⁴ mutants exhibit a slight but consistent reduction in neurotransmitter release (measured by intracellular recordings from muscle 6 of segment A3). These mutants also show a small reduction in synaptic bouton number. Gross synaptic ultrastructure (examined in 3rd instar larval muscles 6 & 7) appears normal apart from a small population of large vesicles distinct from and unlike synaptic vesicles, and occasional aberrant cytoplasmic electron-dense structures ('T-bodies') that can cluster synaptic vesicles. In the active zones of gbb¹/gbb⁴ mutants there are intermittent detachments between pre- and postsynaptic membranes. Mutant boutons are approximately the same size as wild-type boutons, but the bouton surface area per active zone is increased. However, the bouton surface area per T bar is decreased compared to wild-type. The wings of gbb¹/gbb⁴ adults lack a posterior crossvein, and have a truncated L5 vein.

(McCabe et al., 2003)

gbb¹/gbb⁴ males raised at 18^oC have significantly smaller testes than normal, with a dramatic reduction in the number of germ cells of all stages, particularly germ line stem cells, spermatogonia and spermatocytes. In the most extreme cases, germ line stem cells, spermatogonia and spermatocytes are completely missing.

(Shivdasani and Ingham, 2003)

gbb¹/gbb⁴ adults lack the posterior crossvein and the distal tips of veins L4 and L5. The overall size of the wing is reduced compared to wild type. gbb³/gbb⁴ adults lack the posterior crossvein, vein L5 is truncated to the base and L4 is truncated distally. The overall size of the wing is reduced compared to wild type.

(Ray and Wharton, 2001)

gbb¹/gbb⁴ flies show wing vein truncations.

(Yu et al., 2000)

Wings small, and more pointed than wild type. In gbb²/gbb⁴, posterior crossveins are absent. Regions of L4 and L5 are lost. The extent of vein loss depends on the strength of the allelic combination. Some mutant combinations show a slight thickening of distal L2 sometimes with ectopic flanking vein material. The eyes of gbb¹/gbb⁴ are reduced in size with 10-20% ommatidia fewer than wild type. Loss of ommatidia is restricted to the ventral portion of the eye. gbb¹/gbb⁴ and gbb⁴/gbb⁴ show extra scutellar and dorsocentral bristles. Ectopic scutellars most often occur in close proximity to the endogenous bristle, but sometimes between the anterior and posterior scutellar bristles.

(Wharton et al., 1999)

Less than 5% of gbb¹/gbb⁴ flies survive to adulthood at 25^oC. Escapers show a reduction of wing veins L4 and L5 and lack the posterior crossvein.

(Haerry et al., 1998)

gbb¹/gbb⁴ animals generally die as larvae, with rare adult escapers (less than 1%). The larvae appear transparent, due to a defect both in the quantity and quality of the fat body, and a dramatic reduction in imaginal tissues. Areas of the brain are also reduced. The larvae develop more slowly than wild-type and never attain wild-type size. Adult escapers have small, misshapen wings, which lack the posterior crossvein, much of wing vein L5, distal portions of vein L4 and the posterior half of the anterior crossvein. There is a loss of intervein material, especially between veins L2 and L3, between L4 and L5 and posterior to L5, resulting in narrow, pointed wings. Thinning of vein L2 and some ectopic vein material in the intervein region flanking vein L2 is common. No abnormalities in position or type of wing bristles are seen along the wing margin, although ectopic margin bristles are often seen along a vein or in intervein tissue in distal regions of the wing. The eyes are smaller than normal and have supernumerary vibrissae, there is an increase in the number of thoracic bristles, and leg segments may be misshapen. Female sterility is also seen. Homozygous adults have a wing phenotype similar to, but less severe than, gbb¹/gbb⁴ escapers; the wing shape is broader and less pointed, more like wild-type, 54% of individuals have a complete anterior crossvein and longitudinal veins L4 and L5 are longer than in gbb¹/gbb⁴ escapers.

(Khalsa et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

gbb⁴ has visible | recessive phenotype, enhanceable by dpp^d12

(Khalsa et al., 1998)

gbb⁴ has visible | recessive phenotype, enhanceable by Df(2L)tkv2

(Khalsa et al., 1998)

gbb⁴ has visible | recessive phenotype, enhanceable by dpp^d5

(Khalsa et al., 1998)

Suppressed by

Statement

Reference

gbb^5I/gbb⁴ has visible | adult stage phenotype, suppressible | partially by scw^UAS.Tag:FLAG/Scer\GAL4^dpp.3KK

(Tauscher et al., 2016)

gbb^5I/gbb⁴ has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^dpp.3KK/scw^{N1Q.UAS.Tag:FLAG}

(Tauscher et al., 2016)

gbb^5I/gbb⁴ has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^dpp.3KK/scw^{N2Q.UAS.Tag:FLAG}

(Tauscher et al., 2016)

gbb^5I/gbb⁴ has visible | adult stage phenotype, suppressible by Scer\GAL4^dpp.3KK/scw^{N1-N2Q.UAS.Tag:FLAG}

(Tauscher et al., 2016)

gbb^5I/gbb⁴ has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^dpp.3KK/gbb::scw^{scwPD.gbbLD.UAS.Tag:FLAG}

(Tauscher et al., 2016)

gbb^5I/gbb⁴ has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^dpp.3KK/gbb::scw^{scwPD.gbbLD+Glc.UAS.Tag:FLAG}

(Tauscher et al., 2016)

Dp(2;2)DTD48, gbb⁴ has visible phenotype, suppressible by Hsap\BMP7^gbb.PF

(Fritsch et al., 2012)

Dp(2;2)DTD48, gbb⁴ has visible phenotype, suppressible by gbb::Hsap\BMP7^BMP7LD.gbb

(Fritsch et al., 2012)

gbb⁴/gbb¹ has visible phenotype, suppressible by Agam\gbb1^gbb.PF

(Fritsch et al., 2010)

gbb⁴/gbb¹ has visible phenotype, suppressible by Agam\gbb2^gbb.PF

(Fritsch et al., 2010)

gbb⁴/gbb¹ has visible phenotype, suppressible by sax¹/Agam\gbb1^gbb.PF/sax²

(Fritsch et al., 2010)

gbb⁴/gbb¹ has visible phenotype, suppressible by sax¹/Agam\gbb2^gbb.PF/sax²

(Fritsch et al., 2010)

Dp(2;2)DTD48, gbb⁴ has visible phenotype, suppressible by scw^gbb.PF

(Fritsch et al., 2010)

gbb^D20/gbb⁴ has lethal phenotype, suppressible | partially by scw^gbb.PF

(Fritsch et al., 2010)

gbb⁴/gbb¹ has lethal phenotype, suppressible | partially by Dp(2;2)B16/Dp(2;2)DTD48

(Ray and Wharton, 2001)

gbb⁴/gbb¹ has lethal phenotype, suppressible | partially by Dp(2;2)DTD48

(Ray and Wharton, 2001)

gbb⁴/gbb¹ has lethal phenotype, suppressible | partially by Dp(2;2)B16

(Ray and Wharton, 2001)

NOT suppressed by

Statement

Reference

gbb⁴/gbb¹ has visible phenotype, non-suppressible by sax¹/scw^gbb.PF/sax²

(Fritsch et al., 2010)

Dp(2;2)DTD48, gbb⁴ has visible phenotype, non-suppressible by scw^gbb.PF/cv⁴³

(Fritsch et al., 2010)

Enhancer of

Statement

Reference

gbb⁴ is an enhancer of partially lethal - majority die phenotype of Neto¹⁰⁹

(Sulkowski et al., 2014)

gbb⁴/gbb[+] is an enhancer of visible | dominant phenotype of sog^EP7

(Araujo et al., 2003)

Suppressor of

Statement

Reference

gbb⁴/gbb¹ is a suppressor of abnormal neuroanatomy phenotype of spict^mut

(Wang et al., 2007)

NOT Suppressor of

Statement

Reference

gbb⁴/gbb[+] is a non-suppressor of visible | dominant phenotype of sog^EP11

(Araujo et al., 2003)

Other

Statement

Reference

Dp(2;2)DTD48, gbb⁴ has visible phenotype

(Fritsch et al., 2012, Fritsch et al., 2010)

Dp(2;2)DTD48, gbb⁴ has viable phenotype

(Fritsch et al., 2010)

gbb⁴/gbb¹, sax⁴ has partially lethal phenotype

(Khalsa et al., 1998)

gbb⁴/gbb¹, sax⁵ has partially lethal phenotype

(Khalsa et al., 1998)

dpp^d12, gbb⁴ has partially lethal phenotype

(Khalsa et al., 1998)

Df(2L)tkv2, gbb⁴ has viable phenotype

(Khalsa et al., 1998)

gbb⁴, sax⁴ has partially lethal phenotype

(Khalsa et al., 1998)

gbb⁴, sax⁵ has partially lethal phenotype

(Khalsa et al., 1998)

Phenotype Manifest In

Enhanced by

Statement

Reference

gbb^5I/gbb⁴ has wing vein L5 phenotype, enhanceable by dpp^d12/dpp[+]

(Bangi and Wharton, 2006)

gbb^5I/gbb⁴ has wing vein L4 phenotype, enhanceable by dpp^d12/dpp[+]

(Bangi and Wharton, 2006)

gbb⁴ has wing vein L4 phenotype, enhanceable by dpp^d12

(Khalsa et al., 1998)

gbb⁴ has anterior crossvein phenotype, enhanceable by dpp^d12

(Khalsa et al., 1998)

gbb⁴ has wing phenotype, enhanceable by Df(2L)tkv2

(Khalsa et al., 1998)

gbb⁴ has anterior crossvein phenotype, enhanceable by dpp^d5

(Khalsa et al., 1998)

gbb⁴ has wing vein L4 phenotype, enhanceable by dpp^d5

(Khalsa et al., 1998)

gbb⁴ has wing vein L2 phenotype, enhanceable by dpp^d12

(Khalsa et al., 1998)

gbb⁴ has wing vein L3 phenotype, enhanceable by dpp^d12

(Khalsa et al., 1998)

gbb⁴ has wing vein L5 phenotype, enhanceable by dpp^d12

(Khalsa et al., 1998)

gbb⁴ has wing cell phenotype, enhanceable by dpp^d12

(Khalsa et al., 1998)

gbb⁴ has submarginal wing cell phenotype, enhanceable by dpp^d12

(Khalsa et al., 1998)

gbb⁴ has second posterior wing cell phenotype, enhanceable by dpp^d12

(Khalsa et al., 1998)

gbb⁴ has discal wing cell phenotype, enhanceable by dpp^d12

(Khalsa et al., 1998)

gbb⁴ has second basal wing cell phenotype, enhanceable by dpp^d12

(Khalsa et al., 1998)

Suppressed by

Statement

Reference

gbb^5I/gbb⁴ has posterior crossvein phenotype, suppressible | partially by scw^UAS.Tag:FLAG/Scer\GAL4^dpp.3KK

(Tauscher et al., 2016)

gbb^5I/gbb⁴ has posterior crossvein phenotype, suppressible | partially by Scer\GAL4^dpp.3KK/scw^{N1Q.UAS.Tag:FLAG}

(Tauscher et al., 2016)

gbb^5I/gbb⁴ has posterior crossvein phenotype, suppressible | partially by Scer\GAL4^dpp.3KK/scw^{N2Q.UAS.Tag:FLAG}

(Tauscher et al., 2016)

gbb^5I/gbb⁴ has posterior crossvein phenotype, suppressible by Scer\GAL4^dpp.3KK/scw^{N1-N2Q.UAS.Tag:FLAG}

(Tauscher et al., 2016)

gbb^5I/gbb⁴ has posterior crossvein phenotype, suppressible | partially by Scer\GAL4^dpp.3KK/gbb::scw^{scwPD.gbbLD.UAS.Tag:FLAG}

(Tauscher et al., 2016)

gbb^5I/gbb⁴ has posterior crossvein phenotype, suppressible | partially by Scer\GAL4^dpp.3KK/gbb::scw^{scwPD.gbbLD+Glc.UAS.Tag:FLAG}

(Tauscher et al., 2016)

Dp(2;2)DTD48, gbb⁴ has posterior crossvein phenotype, suppressible by Hsap\BMP7^gbb.PF

(Fritsch et al., 2012)

Dp(2;2)DTD48, gbb⁴ has posterior crossvein phenotype, suppressible by gbb::Hsap\BMP7^BMP7LD.gbb

(Fritsch et al., 2012)

gbb⁴/gbb¹ has wing vein phenotype, suppressible by Agam\gbb1^gbb.PF

(Fritsch et al., 2010)

gbb⁴/gbb¹ has wing vein phenotype, suppressible by Agam\gbb2^gbb.PF

(Fritsch et al., 2010)

gbb⁴/gbb¹ has wing vein phenotype, suppressible by sax¹/Agam\gbb1^gbb.PF/sax²

(Fritsch et al., 2010)

gbb⁴/gbb¹ has wing vein phenotype, suppressible by sax¹/Agam\gbb2^gbb.PF/sax²

(Fritsch et al., 2010)

Dp(2;2)DTD48, gbb⁴ has posterior crossvein phenotype, suppressible by scw^gbb.PF

(Fritsch et al., 2010)

gbb^D20/gbb⁴ has posterior crossvein phenotype, suppressible by scw^gbb.PF

(Fritsch et al., 2010)

gbb^D20/gbb⁴ has wing vein L5 phenotype, suppressible by scw^gbb.PF

(Fritsch et al., 2010)

gbb^D20/gbb⁴ has male germline stem cell | heat sensitive phenotype, suppressible | partially by Dad[+]/Dad^P1883

(Kawase et al., 2004)

NOT suppressed by

Statement

Reference

gbb⁴/gbb¹ has wing vein phenotype, non-suppressible by sax¹/scw^gbb.PF/sax²

(Fritsch et al., 2010)

Dp(2;2)DTD48, gbb⁴ has posterior crossvein phenotype, non-suppressible by scw^gbb.PF/cv⁴³

(Fritsch et al., 2010)

Enhancer of

Statement

Reference

gbb⁴/gbb[+] is an enhancer of wing vein phenotype of sog^EP7

(Araujo et al., 2003)

gbb⁴ is an enhancer of posterior crossvein phenotype of cv-2^k03511/cv-2^225-3

(Conley et al., 2000)

Suppressor of

Statement

Reference

gbb⁴/gbb¹ is a suppressor of embryonic/larval neuromuscular junction phenotype of spict^mut

(Wang et al., 2007)

gbb⁴/gbb⁴ is a suppressor of wing vein | ectopic | somatic clone | cell non-autonomous phenotype of sax⁴

(Bangi and Wharton, 2006)

NOT Suppressor of

Statement

Reference

gbb⁴/gbb[+] is a non-suppressor of wing vein phenotype of sog^EP11

(Araujo et al., 2003)

Other

Statement

Reference

Dp(2;2)DTD48, gbb⁴ has posterior crossvein phenotype

(Fritsch et al., 2012, Fritsch et al., 2010)

bam^hs.PO, gbb⁴/gbb^D4 has follicle cell & germarium | conditional - heat sensitive phenotype

(Kirilly et al., 2005)

bam^hs.PO, gbb⁴/gbb^D4 has germarium | heat sensitive phenotype

(Kirilly et al., 2005)

bam^hs.PO, gbb^D20/gbb⁴ has follicle cell & germarium | conditional - heat sensitive phenotype

(Kirilly et al., 2005)

bam^hs.PO, gbb^D20/gbb⁴ has germarium | heat sensitive phenotype

(Kirilly et al., 2005)

dpp^d12, gbb⁴/gbb¹ has prothoracic tarsal segment | male phenotype

(Khalsa et al., 1998)

dpp^d12, gbb⁴ has prothoracic tarsal segment | male phenotype

(Khalsa et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

The loss of posterior crossvein characteristic for gbb^5I/gbb⁴ can be partially rescued (with varying efficiency) by Scer\GAL4^dpp.3KK-driven expression of any of the following: scw^{Scer\UAS.T:Zzzz\FLAG}, scw^{N1Q.Scer\UAS.T:Zzzz\FLAG}, scw^{N2Q.Scer\UAS.T:Zzzz\FLAG}, gbb::scw^{scwPD.gbbLD.Scer\UAS.T:Zzzz\FLAG} or gbb::scw^{scwPD.gbbLD+Glc.Scer\UAS.T:Zzzz\FLAG} and fully rescued by the expression of scw^{N1-N2Q.Scer\UAS.T:Zzzz\FLAG} in the mutant background.

(Tauscher et al., 2016)

gbb⁴/Dp(2;2)DTD48 gbb⁴ flies show loss of the posterior crossvein.

(Fritsch et al., 2012)

A single copy of scw^gbb.PF partially rescues the lethality of gbb⁴/gbb^D20 animals (34% of the expected number of adults are recovered) and the loss of posterior crossvein and truncation of L5 that are seen in gbb⁴/gbb^D20 adult escapers are partially restored. Two copies of scw^gbb.PF rescue the posterior crossvein and vein L5 phenotypes to nearly wild type.

gbb⁴ homozygotes are viable in the presence of Dp(2;2)DTD48, but the flies completely lack the posterior crossvein.

The loss of posterior crossvein which is seen in Dp(2;2)DTD48, gbb⁴/gbb⁴ flies is rescued by a single copy of scw^gbb.PF.

The ability of scw^gbb.PF to rescue the loss of posterior crossvein which is seen in Dp(2;2)DTD48, gbb⁴/gbb⁴ flies is completely suppressed in a cv⁴³ background.

scw^gbb.PF cannot rescue the wing vein defects of gbb¹/gbb⁴ flies in a sax¹/sax² background.

(Fritsch et al., 2010)

spict^mut neuromuscular junction overgrowth phenotypes are fully suppressed in gbb¹/gbb⁴ mutants. The synaptic undergrowth phenotypes in larvae homozygous for spict^mut in a gbb¹/gbb⁴ background are indistinguishable from that of gbb¹/gbb⁴ mutants alone.

(Wang et al., 2007)

gbb⁴/gbb^5I, dpp^d12/+ flies show an enhancement of the loss of wing vein L4 seen in gbb⁴/gbb^5I flies. 3% of gbb⁴/gbb^5I, dpp^d12/+ flies show severe truncation of wing vein L5, a phenotype not seen in the single mutants.

(Bangi and Wharton, 2006)

sax⁴ clones induced in a gbb⁴ background that are posterior to L5 or anterior to L2 never lead to ectopic wing vein formation.

(Bangi and Wharton, 2006)

bam^hs.PO animals cultured at 18^oC and heat shocked as late stage pupae (37^oC, 4 times for 2 hours each with an 8-12 hour interval) produce adult females with agametic germaria. If these animals also carry the temperature sensitive allelic combination gbb⁴/gbb^D4 and are shifted to 29^oC (the non-permissive temperature) for 10 days, the majority of germaria disintegrate. Among remaining germaria, less than half contain follicle cells but all contain terminal filament cells. In identical experiments using gbb⁴/gbb^D20, no follicle cells remain, although the resulting germaria do contain terminal filament cells.

(Kirilly et al., 2005)

gbb⁴/gbb^D20 males raised to adulthood at 18^oC and then shifted to 22^oC for 4 days have an average of 2.4 germline stem cells per testis. This is increased to 4.8 by the presence of Dad^P1883/+.

(Kawase et al., 2004)

The addition of gbb⁴ to cv-2^225-3/cv-2^k03511 animals produces an enhancement of the posterior crossvein phenotype seen in these flies.

(Conley et al., 2000)

The homozygous gbb⁴ phenotype is enhanced by dpp^d5; gbb⁴ dpp^d5; gbb⁴ + flies show a significant loss of wing vein L4 and a more frequent loss of the anterior crossvein than gbb⁴ +; gbb⁴ + flies. The gbb⁴/gbb⁴ and gbb¹/gbb⁴ phenotypes are dominantly enhanced by dpp^d12; there is a greater loss of wing vein L4, wing vein L2 and the anterior crossvein than in gbb single mutant flies. There is also loss of intervein tissue, especially between veins L2 and L3 and between veins L4 and L5, resulting in a reduction in the size of the wing. A gap at the distal end of vein L3 is seen in 3% of cases. A reduction in the loss of wing vein L5 is seen in these flies. Viability is reduced. gbb¹ dpp^d12/gbb⁴ + flies show truncations and/or fusions of the distal-most tarsal segments of the male prothoracic leg. Addition of Df(2L)tkv2 into gbb¹/gbb⁴ flies results in a more severe wing phenotype but less severe lethality. The viability of gbb¹/gbb⁴ flies is dominantly reduced by sax⁴ or sax⁵.

(Khalsa et al., 1998)

Xenogenetic Interactions

Statement

Reference

The loss of posterior crossvein seen in gbb⁴/Dp(2;2)DTD48 gbb⁴ flies is completely rescued by expression of either Hsap\BMP7^gbb.PF or gbb::Hsap\BMP7^BMP7LD.gbb, but is not rescued by expression of Hsap\BMP7^R292G.gbb.

(Fritsch et al., 2012)

Agam\gbb1^gbb.PF and Agam\gbb2^gbb.PF each completely rescue the wing vein defects of gbb¹/gbb⁴ animals.

Agam\gbb1^gbb.PF and Agam\gbb2^gbb.PF each partially rescue the wing vein defects of gbb¹/gbb⁴ animals in a sax¹/sax² background; partial rescue of the posterior crossvein is seen and in addition loss of the distal tips of vein L5 and loss of the distal quarter of vein L4 are seen.

(Fritsch et al., 2010)

Complementation and Rescue Data

Rescued by

gbb⁴ is rescued by Scer\GAL4^{VP16.nanos.UTR}/gbb^UAS.cKa

(Shivdasani and Ingham, 2003)

Partially rescued by

gbb⁴/gbb¹ is partially rescued by Scer\GAL4^how-24B/gbb^UAS.cKa

(Khalsa et al., 1998)

gbb⁴/gbb¹ is partially rescued by gbb^UAS.cKa/Scer\GAL4^hs.PB

(Khalsa et al., 1998)

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Mutant

Wild-type

Stocks (1)

Bloomington

98345

gbb⁴

Notes on Origin

Discoverer

Induced with: a second site lethal mutation that has subsequently been removed by recombination.

(Wharton et al., 1999)

Comments

Hemizygotes over Df(2R)b23 show a more severe phenotype than homozygotes. The gbb alleles fall into a phenotypic series. Starting with the most severe alleles: gbb¹ = gbb² > gbb³ > gbb⁴.

(Wharton et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ab-4

(Wharton et al., 1999)

gbb-60A⁴

(Araujo et al., 2003, Khalsa et al., 1998)

gbb⁴

(Hossain et al., 2023, Kamimura et al., 2019, Kanai et al., 2018, Anderson and Wharton, 2017, Tauscher et al., 2016, Liu et al., 2014, Sulkowski et al., 2014, Fritsch et al., 2012, Matsuda and Shimmi, 2012, Pilgram et al., 2011, Quijano et al., 2011, Fritsch et al., 2010, Christoforou et al., 2008, Serpe et al., 2008, Wang et al., 2007, Bangi and Wharton, 2006, Bangi and Wharton, 2006, Kirilly et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (31)

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