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General Information
Symbol
Dmel\cnksag32-3
Species
D. melanogaster
Name
FlyBase ID
FBal0095216
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

A17415826T

Amino acid change:

K1141term | cnk-PA; K1141term | cnk-PB

Reported amino acid change:

K1141term

Comment:

The mutation is reported as 2R:T17415826A leading to a nonsense mutation in K1141. The presumed typo should have read 2R:A17415826T .

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: K1141term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

cnksag32-3/Df(2R)BSC161 embryos derived from mothers bearing cnksag32-3 mutant germline clones and thus lacking both maternal and zygotic cnk show a complete loss of muscle founder cells at late embryonic stages and impaired tracheal development.

cnksag13L/cnksag32-3;cnkfTRG1248 rescue flies survive to adulthood but display rough eye phenotype.

Mutants die as late pupae with small rough eyes. Mutants contain only about 50% of the normal number of ommatidia. Third instar larval eye discs are smaller in size. In these discs the first mitotic wave is unaffected, but there is an approximately 50-50% reduction of the number of mitotic cells in the second mitotic wave. Within homozygous sag32-3 somatic clones in the eye, ommatidia lack the inner R7, and two or three of the outer photoreceptors. The mutant cells lack the pigment granules that are present in wild-type cells at the base of the rhabdomeres. In addition, the mutant photoreceptors form smaller rhabdomeres compared to wild-type. sagX-10/sag32-3 animals are viable but have rough eyes due to disorganised ommatidial arrays and reduced numbers of photoreceptor cells.

Homozygotes die at the late pupal stage. Pharate adults have rough eyes with only approximately 50% of the normal number of ommatidia. Third instar larval eye discs are smaller than wild-type.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference

sag[+]/cnksag32-3 is an enhancer of lethal | recessive phenotype of Raf12

NOT Suppressor of
Statement
Reference

Src42ASu(Raf)1-1/cnksag32-3 is a non-suppressor of lethal phenotype of Raf1

Phenotype Manifest In
Suppressor of
Statement
Reference

sag[+]/cnksag32-3 is a suppressor of phenotype of Su(Raf)43B43B

sag[+]/cnksag32-3 is a suppressor of phenotype of Dsor1Su34B

Additional Comments
Genetic Interactions
Statement
Reference

Dominantly suppresses the ability of Src42ASu(phl)1-1, Su(Raf)43B43B or Dsor1Su34B to suppress the lethality of phl1/Y flies. Dominantly enhances the lethality of phl12.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The lethality of either cnksag32-3/Df(2R)BSC161 or cnksag13L/cnksag32-3 mutants (but not cnksag13L or cnksag32-3 homozygotes) is rescued by combination with cnkfTRG1248 but the adult cnksag13L/cnksag32-3;cnkfTRG1248 rescue flies display rough eye phenotype.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Selected as: a mutation that dominantly suppresses the ability of Src42ASu(phl)1-1 to suppress the lethality of phl1/Y flies.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (3)