FlyBase Allele Report: Dmel\cnk[sag32-3]

General Information

Symbol

Dmel\cnk^sag32-3

Species

D. melanogaster

Name

FlyBase ID

FBal0095216

Feature type

allele

Associated gene

Dmel\cnk

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Zhang and Lu, 2000)

Mutagen

ethyl methanesulfonate

(Zhang et al., 1999)

Progenitor genotype

Cytology

Description

Amino acid replacement: K1141term.

(Wolfstetter et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:17,415,826..17,415,826 [-]

Nucleotide change:

A17415826T

Amino acid change:

K1141term | cnk-PA; K1141term | cnk-PB

Reported amino acid change:

K1141term

Comment:

The mutation is reported as 2R:T17415826A leading to a nonsense mutation in K1141. The presumed typo should have read 2R:A17415826T .

(Wolfstetter et al., 2017)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

eye

(Zhang and Lu, 2000, Zhang et al., 1999)

eye (with cnk^sag13L), with cnk^{fTRG01248.sfGFP-TVPTBF}

(Wolfstetter et al., 2017)

eye (with cnk^X-10)

(Zhang and Lu, 2000)

eye disc

(Zhang et al., 1999)

eye photoreceptor cell (with cnk^X-10)

(Zhang and Lu, 2000)

eye photoreceptor cell | somatic clone

(Zhang and Lu, 2000)

mitotic cell cycle & eye disc

(Zhang and Lu, 2000)

muscle founder cell | embryonic stage | maternal effect (with Df(2R)BSC161)

(Wolfstetter et al., 2017)

ommatidium

(Zhang and Lu, 2000, Zhang et al., 1999)

ommatidium (with cnk^X-10)

(Zhang and Lu, 2000)

photoreceptor cell R7 | somatic clone

(Zhang and Lu, 2000)

pigment granule | somatic clone

(Zhang and Lu, 2000)

rhabdomere | somatic clone

(Zhang and Lu, 2000)

tracheal section | embryonic stage | maternal effect (with Df(2R)BSC161)

(Wolfstetter et al., 2017)

Detailed Description

Statement

Reference

cnk^sag32-3/Df(2R)BSC161 embryos derived from mothers bearing cnk^sag32-3 mutant germline clones and thus lacking both maternal and zygotic cnk show a complete loss of muscle founder cells at late embryonic stages and impaired tracheal development.

cnk^sag13L/cnk^sag32-3;cnk^fTRG1248 rescue flies survive to adulthood but display rough eye phenotype.

(Wolfstetter et al., 2017)

Mutants die as late pupae with small rough eyes. Mutants contain only about 50% of the normal number of ommatidia. Third instar larval eye discs are smaller in size. In these discs the first mitotic wave is unaffected, but there is an approximately 50-50% reduction of the number of mitotic cells in the second mitotic wave. Within homozygous sag^32-3 somatic clones in the eye, ommatidia lack the inner R7, and two or three of the outer photoreceptors. The mutant cells lack the pigment granules that are present in wild-type cells at the base of the rhabdomeres. In addition, the mutant photoreceptors form smaller rhabdomeres compared to wild-type. sag^X-10/sag^32-3 animals are viable but have rough eyes due to disorganised ommatidial arrays and reduced numbers of photoreceptor cells.

(Zhang and Lu, 2000)

Homozygotes die at the late pupal stage. Pharate adults have rough eyes with only approximately 50% of the normal number of ommatidia. Third instar larval eye discs are smaller than wild-type.

(Zhang et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

sag[+]/cnk^sag32-3 is an enhancer of lethal | recessive phenotype of Raf¹²

(Zhang et al., 1999)

NOT Suppressor of

Statement

Reference

Src42A^Su(Raf)1-1/cnk^sag32-3 is a non-suppressor of lethal phenotype of Raf¹

(Zhang and Lu, 2000)

Phenotype Manifest In

Suppressor of

Statement

Reference

sag[+]/cnk^sag32-3 is a suppressor of phenotype of Dsor1^Su34B

(Zhang et al., 1999)

sag[+]/cnk^sag32-3 is a suppressor of phenotype of Su(Raf)43B^43B

(Zhang et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Dominantly suppresses the ability of Src42A^Su(phl)1-1, Su(Raf)43B^43B or Dsor1^Su34B to suppress the lethality of phl¹/Y flies. Dominantly enhances the lethality of phl¹².

(Zhang et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

cnk^sag13L/cnk^sag32-3 is rescued by cnk^{fTRG01248.sfGFP-TVPTBF}

(Wolfstetter et al., 2017)

cnk^sag32-3/Df(2R)BSC161 is rescued by cnk^{fTRG01248.sfGFP-TVPTBF}

(Wolfstetter et al., 2017)

Not rescued by

cnk^sag32-3 is not rescued by cnk^{fTRG01248.sfGFP-TVPTBF}

(Wolfstetter et al., 2017)

Comments

The lethality of either cnk^sag32-3/Df(2R)BSC161 or cnk^sag13L/cnk^sag32-3 mutants (but not cnk^sag13L or cnk^sag32-3 homozygotes) is rescued by combination with cnk^fTRG1248 but the adult cnk^sag13L/cnk^sag32-3;cnk^fTRG1248 rescue flies display rough eye phenotype.

(Wolfstetter et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a mutation that dominantly suppresses the ability of Src42A^Su(phl)1-1 to suppress the lethality of phl¹/Y flies.

(Zhang et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

cnk^sag32-3

(Wolfstetter et al., 2017)

sag^32-3

(Wolfstetter et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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