FlyBase Allele Report: Dmel\cnk[sag13L]

cnk^sag13L/Df(2R)BSC161 embryos derived from mothers bearing cnk^sag13L mutant germline clones and thus lacking both maternal and zygotic cnk show a complete loss of muscle founder cells at stage 11/12.

cnk^sag13L/cnk^sag32-3;cnk^fTRG1248 rescue flies survive to adulthood but display rough eye phenotype.

(Wolfstetter et al., 2017)

Mutants die as late pupae with small rough eyes. Mutants contain only about 50% of the normal number of ommatidia. Third instar larval eye discs are smaller in size. In these discs the first mitotic wave is unaffected, but there is an approximately 50-50% reduction of the number of mitotic cells in the second mitotic wave. sag^X-10/sag^13L animals are viable but have rough eyes due to disorganised ommatidial arrays and reduced numbers of photoreceptor cells. 19% of sag^X-10/sag^13L ommatidia lack R7 cells. In sag^13L mutant eye discs, photoreceptor cells R1, R6 and R7 are missing in ommatidial preclusters. The numbers of cone and pigment cells are also dramatically reduced but not eliminated. Many ommatidia contain only one cone cell wrapped around by one primary pigment cell. The number of secondary and tertiary pigment cells are also reduced. The remaining cone and pigment cells vary in numbers from one ommatidium to the next. Within homozygous sag^13L somatic clones in the eye, ommatidia lack the inner R7, and photoreceptors R1 and R6. Photoreceptors R2 - R5 although present, are smaller than wild-type. The mutant cells lack the pigment granules that are present in wild-type cells at the base of the rhabdomeres. In addition, the mutant photoreceptors form smaller rhabdomeres compared to wild-type.

(Zhang and Lu, 2000)

Homozygotes die at the late pupal stage. Pharate adults have rough eyes with only approximately 50% of the normal number of ommatidia. The ommatidia are disorganised and 2 to 3 photoreceptor cells per ommatidium are missing. Degeneration of photoreceptor cells is seen. Third instar larval eye discs are smaller than wild-type. No wild-type ommatidia are found within homozygous clones in the eye; 75% lack the R7 cell and 94% lack 1 to 3 outer photoreceptors. No degeneration of the mutant photoreceptor cells is seen.

(Zhang et al., 1999)

External Data

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Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

cnk^sag13L/sag[+] is an enhancer of visible phenotype of rl^13R/rl¹

(Zhang et al., 1999)

cnk^sag13L/sag[+] is an enhancer of lethal | recessive phenotype of Raf¹²

(Zhang et al., 1999)

NOT Suppressor of

Statement

Reference

cnk^sag13L/Src42A^Su(Raf)1-1 is a non-suppressor of lethal phenotype of Raf¹

(Zhang and Lu, 2000)

Other

Statement

Reference

cnk^sag13L, rl^41-1 has visible | dominant phenotype

(Zhang et al., 1999)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

cnk^sag13L has photoreceptor cell R1 | somatic clone phenotype, non-enhanceable by Ras85D^V12.sev

(Zhang and Lu, 2000)

cnk^sag13L has photoreceptor cell R6 | somatic clone phenotype, non-enhanceable by Ras85D^V12.sev

(Zhang and Lu, 2000)

cnk^sag13L has photoreceptor cell R7 | somatic clone phenotype, non-enhanceable by Ras85D^V12.sev

(Zhang and Lu, 2000)

cnk^sag13L has eye photoreceptor cell | somatic clone phenotype, non-enhanceable by Ras85D^V12.sev

(Zhang and Lu, 2000)

cnk^sag13L has pigment granule | somatic clone phenotype, non-enhanceable by Ras85D^V12.sev

(Zhang and Lu, 2000)

cnk^sag13L has rhabdomere | somatic clone phenotype, non-enhanceable by Ras85D^V12.sev

(Zhang and Lu, 2000)

cnk^sag13L has ommatidium | somatic clone phenotype, non-enhanceable by Ras85D^V12.sev

(Zhang and Lu, 2000)

Suppressed by

Statement

Reference

cnk^sag13L has ommatidium | somatic clone phenotype, suppressible by aop^yan-1

(Zhang and Lu, 2000)

cnk^sag13L has photoreceptor cell R1 | somatic clone phenotype, suppressible by aop^yan-1

(Zhang and Lu, 2000)

cnk^sag13L has photoreceptor cell R6 | somatic clone phenotype, suppressible by aop^yan-1

(Zhang and Lu, 2000)

cnk^sag13L has photoreceptor cell R7 | somatic clone phenotype, suppressible by aop^yan-1

(Zhang and Lu, 2000)

cnk^sag13L has eye photoreceptor cell | somatic clone phenotype, suppressible by aop^yan-1

(Zhang and Lu, 2000)

cnk^X-10/cnk^sag13L has photoreceptor cell R7 | ectopic phenotype, suppressible by rl^D334N.hs.sev

(Zhang and Lu, 2000)

NOT suppressed by

Statement

Reference

cnk^sag13L has ommatidium | somatic clone phenotype, non-suppressible by Ras85D^V12.sev

(Zhang and Lu, 2000)

cnk^sag13L has photoreceptor cell R1 | somatic clone phenotype, non-suppressible by Ras85D^V12.sev

(Zhang and Lu, 2000)

cnk^sag13L has photoreceptor cell R6 | somatic clone phenotype, non-suppressible by Ras85D^V12.sev

(Zhang and Lu, 2000)

cnk^sag13L has photoreceptor cell R7 | somatic clone phenotype, non-suppressible by Ras85D^V12.sev

(Zhang and Lu, 2000)

cnk^sag13L has eye photoreceptor cell | somatic clone phenotype, non-suppressible by Ras85D^V12.sev

(Zhang and Lu, 2000)

cnk^sag13L has pigment granule | somatic clone phenotype, non-suppressible by Ras85D^V12.sev

(Zhang and Lu, 2000)

cnk^sag13L has rhabdomere | somatic clone phenotype, non-suppressible by Ras85D^V12.sev

(Zhang and Lu, 2000)

Enhancer of

Statement

Reference

cnk^sag13L/sag[+] is an enhancer of photoreceptor cell R7 phenotype of Ras85D^N17.sev

(Zhang and Lu, 2000)

cnk^sag13L is an enhancer of ommatidium phenotype of Sos^JC2, sev⁶

(Zhang and Lu, 2000)

cnk^sag13L/sag[+] is an enhancer of eye phenotype of rl^13R/rl¹

(Zhang et al., 1999)

Suppressor of

Statement

Reference

cnk^X-10/cnk^sag13L is a suppressor of photoreceptor cell R7 | ectopic phenotype of rl^D334N.hs.sev

(Zhang and Lu, 2000)

cnk^X-10/cnk^sag13L is a suppressor of ommatidium phenotype of rl^D334N.hs.sev

(Zhang and Lu, 2000)

cnk^sag13L/sag[+] is a suppressor | partially of photoreceptor cell R7 | ectopic phenotype of Ras85D^{V12.ΔCAAX.sev}

(Zhang and Lu, 2000)

cnk^sag13L/sag[+] is a suppressor of phenotype of Dsor1^Su34B

(Zhang et al., 1999)

cnk^sag13L/sag[+] is a suppressor of photoreceptor neuron phenotype of Ras85D^V12.sev

(Zhang et al., 1999)

cnk^sag13L/sag[+] is a suppressor of photoreceptor cell R7 | ectopic phenotype of Ras85D^V12.sev

(Zhang et al., 1999)

cnk^sag13L/sag[+] is a suppressor of phenotype of Src42A^Su(Raf)1-1

(Zhang et al., 1999)

cnk^sag13L/sag[+] is a suppressor of phenotype of Su(Raf)43B^43B

(Zhang et al., 1999)

Other

Statement

Reference

cnk^sag13L, rl^41-1 has ommatidium phenotype

(Zhang et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

In Ras85D^V12.sev;sag^13L double mutant somatic clones, exhibits an ommatidial phenotype no different from the phenotype seen in sag^13L clones. In sag^X-10/sag^13L; rl^D334N.hs.sev flies, 99% of ommatidia contain one R7 cell. In 90% of aop^yan-1/aop^yan-1, sag^13L/+ double mutant clones in the eye, there are the normal complement of photoreceptor cells.

(Zhang and Lu, 2000)

Dominantly suppresses the ability of Src42A^Su(phl)1-1, Su(Raf)43B^43B or Dsor1^Su34B to suppress the lethality of phl¹/Y flies. Dominantly enhances the eye phenotype of rl¹/rl^13R flies. rl^41-1 sag^13L double heterozygotes have disorganised ommatidia, although the eyes of either single heterozygote are normal. Dominantly suppresses the extra R7 photoreceptor cell phenotype of flies carrying Ras85D^V12.sev. Dominantly enhances the lethality of phl¹².

(Zhang et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

cnk^sag13L/Df(2R)BSC161 is rescued by cnk^{fTRG01248.sfGFP-TVPTBF}

(Wolfstetter et al., 2017)

cnk^sag13L/cnk^sag32-3 is rescued by cnk^{fTRG01248.sfGFP-TVPTBF}

(Wolfstetter et al., 2017)

Not rescued by

cnk^sag13L is not rescued by cnk^{fTRG01248.sfGFP-TVPTBF}

(Wolfstetter et al., 2017)

Comments

The lethality of either cnk^14C/Df(2R)BSC161 or cnk^sag13L/cnk^sag32-3 mutants (but not cnk^sag13L or cnk^sag32-3 homozygotes) is rescued by combination with cnk^fTRG1248 but the adult cnk^sag13L/cnk^sag32-3;cnk^fTRG1248 rescue flies display rough eye phenotype.

(Wolfstetter et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a mutation that dominantly suppresses the ability of Src42A^Su(phl)1-1 to suppress the lethality of phl¹/Y flies. Isolation: derived from the 'Su^Su(Raf)113 chromosome (which dominantly suppresses the ability of Src42A^Su(phl)1-1 to suppress the lethality of phl¹/Y flies) which contained two mutations; sag^13L and rl^13R. When separated by recombination, sag^13L showed all the suppressor of Src42A^Su(phl)1-1 activity, and rl^13R showed no suppressor of Src42A^Su(phl)1-1 activity.

(Zhang et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

cnk^sag13L

(Wolfstetter et al., 2017)

sag^13L

(Wolfstetter et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (3)

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