FlyBase Allele Report: Dmel\gig[193]

General Information

Symbol

Dmel\gig¹⁹³

Species

D. melanogaster

Name

FlyBase ID

FBal0095407

Feature type

allele

Associated gene

Dmel\gig

Associated Insertion(s)

Carried in Construct

Also Known As

Tsc2¹⁹³

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

X ray

Nature of the Allele

Allele class

Mutagen

X ray

(Ito and Rubin, 1999)

Progenitor genotype

Cytology

Description

166 bp deletion and 10bp insertion that causes an in frame deletion of 56 amino acids from amino acid 472 to 527 and the addition of four amino acids.

(Ito and Rubin, 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3L:20,134,220..20,134,385 [+]

Comment:

A 166 bp deletion and 10bp insertion that causes an in frame deletion of 56 amino acids from amino acid 472 to 527 and the addition of four amino acids. Position of mutation on reference sequence inferred by FlyBase curator based on author statement.

(Ito and Rubin, 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage (with gig¹⁰⁹)

(Zhang et al., 2006)

lethal - all die before end of larval stage | recessive

(Ito and Rubin, 1999)

melanotic mass phenotype

(Ito and Rubin, 1999)

some die during larval stage (with gig¹⁰⁹)

(Zhang et al., 2006)

some die during larval stage | recessive

(Ito and Rubin, 1999)

visible | recessive

(Ito and Rubin, 1999)

Phenotype Manifest In

eye

(Ito and Rubin, 1999)

ommatidium

(Ito and Rubin, 1999)

wing

(Ito and Rubin, 1999)

wing margin bristle

(Ito and Rubin, 1999)

Detailed Description

Statement

Reference

Cells in mutant clones in the eye and wing are larger than wild type siblings.

(Ito and Rubin, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

gig¹⁹³/gig¹⁰⁹ has lethal | larval stage phenotype, suppressible | partially by mTor^V2148D/Tor[+]

(Zhang et al., 2006)

gig¹⁹³/gig¹⁰⁹ has lethal | larval stage phenotype, suppressible | partially by Tor[+]/mTor^W1251R

(Zhang et al., 2006)

gig¹⁹³/gig¹⁰⁹ has lethal | larval stage phenotype, suppressible | partially by mTor^E161K/Tor[+]

(Zhang et al., 2006)

gig¹⁹³/gig¹⁰⁹ has lethal | larval stage phenotype, suppressible | partially by Tor[+]/mTor^ΔP

(Zhang et al., 2006)

gig¹⁹³/gig¹⁰⁹, mTor^+t9.4 has lethal | larval stage phenotype, suppressible | partially by mTor^2L1/Tor[+]

(Zhang et al., 2006)

gig¹⁹³/gig¹⁰⁹ has lethal | larval stage phenotype, suppressible | partially by mTor^2L1/Tor[+]

(Zhang et al., 2006)

gig¹⁹³/gig¹⁰⁹ has lethal | larval stage phenotype, suppressible | partially by Tor[+]/mTor^A948V

(Zhang et al., 2006)

NOT suppressed by

Statement

Reference

gig¹⁹³/gig¹⁰⁹, mTor^+t9.4 has lethal | larval stage phenotype, non-suppressible by Tor[+]/mTor^ΔP

(Zhang et al., 2006)

gig¹⁹³/gig¹⁰⁹ has lethal | larval stage phenotype, non-suppressible by Tor[+]/mTor^EP2353

(Zhang et al., 2006)

gig¹⁹³/gig¹⁰⁹ has lethal | larval stage phenotype, non-suppressible by Tor[+]/mTor^k17004

(Zhang et al., 2006)

gig¹⁹³/gig¹⁰⁹ has lethal | larval stage phenotype, non-suppressible | partially by Tor[+]/mTor^ΔP

(Zhang et al., 2006)

gig¹⁹³/gig¹⁰⁹ has lethal phenotype, non-suppressible by Tor[+]/mTor^G2256D

(Zhang et al., 2006)

gig¹⁹³/gig¹⁰⁹ has lethal | larval stage phenotype, non-suppressible by Tor[+]/mTor^G2256D

(Zhang et al., 2006)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The percentage of gig¹⁰⁹/gig¹⁹³ mutants that survive to adult stages is increased from 0 to around 30% when flies are also Tor^E161K/+, to around 45% when flies are also Tor^A948V/+ or Tor^W1251R/+ and to 60% when they are Tor^2L1/+. Around 2% of Tor^V2148D/+; gig¹⁰⁹/gig¹⁹³ flies survive to adulthood. Around 60% of gig¹⁰⁹/gig¹⁹³; Tor^ΔP/+ flies survive to the pupal, but not the adult stage. However, one copy of the Tor^+t9.4 transgene blocks the ability of Tor^ΔP/+ to rescue gig¹⁰⁹/gig¹⁹³ flies to pupal viability. The Tor^+t9.4 transgene reduces the amount of Tor^2L1/+; gig¹⁰⁹/gig¹⁹³ flies that survive to the pupal stage from around 85% to around 65%.