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General Information
Symbol
Dmel\gclrev390
Species
D. melanogaster
Name
FlyBase ID
FBal0095410
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
gclΔ
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the P{lacW}cmp44Epngcl element, resulting in a deletion that removes the 3' end of the gcl transcription unit, including the entire gcl coding region.

Caused by aberration
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

gclrev390 homozygotes and gclrev390/gclE100K transheterozygotes present severe decreases in the number of primordial germ cells in the early embryo (i.e. mitotic cycles 12/13), as compared to wild-type and heterozygous controls; gclrev390 heterozygotes do not display any obvious dominant defects in fertility, as shown by the insignificant differences in progression through oogenesis and in laid eggs, as compared to controls.

In gclrev390 embryos, the normal transcriptional quiescence seen at nuclear cycle 10 is not seen.

When transformed with the cmp44E rescue construct cmp44E+t8.5, flies homozygous for Df(2R)cmp44E-rev390 lack any visible phenotypes. Mothers homozygous for Df(2R)cmp44E-rev390, with one copy of cmp44E+t8.5, produce embryos that hatch normally. At the blastoderm stage, however, about half lack any germ cells and the remainder have fewer pole cells than control embryos. At stage 14 about 2/3 of embryos lack pole cells and the pole cell survival rate is much lower than in controls - only about 2/5 of pole cells formed at the blastoderm stage successfully reach the embryonic gonad. The proportion of fertile adults match the proportion of embryos that have at least one pole cell at stage 14. The loss of pole cells appears to occur at Stage 10. Examination of earlier stages reveals that 1/5 embryos fail to form detectable pole buds.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Suppressed by
NOT suppressed by
NOT Suppressor of
Statement
Reference

gclrev390/gcl[+] is a non-suppressor of embryonic head phenotype of tsl4/tsl3

gclrev390/gcl[+] is a non-suppressor of embryonic telson phenotype of tsl4/tsl3

gclrev390/gcl[+] is a non-suppressor of primordial germ cell | embryonic stage phenotype of tsl4/tsl3

gclrev390/gclrev390 is a non-suppressor of embryonic head phenotype of tsl4/tsl3

gclrev390/gclrev390 is a non-suppressor of embryonic telson phenotype of tsl4/tsl3

Other
Additional Comments
Genetic Interactions
Statement
Reference

gclrev390, Cul3gft2 double heterozygotes present significant decreases in the number of primordial germ cells in the early embryo (i.e. mitotic cycles 12/13), as compared to wild-type and single heterozygous controls.

The decreased primordial germ cells in gclrev390 homozygous early embryos (i.e. mitotic cycles 12/13) is fully suppressed by tsl3/tsl4 transheterozygosity or by the Scer\GAL4mat.αTub67C.T:Hsim\VP16-driven expression of torHMS00021, torGL00222 or torGLV21002, but not by tsl3 or tsl4 heterozygosity, or by Scer\GAL4mat.αTub67C.T:Hsim\VP16-driven expression of Dsor1GLV21003, Dsor1HMS00145, rlGL00215 or rlHMS00173. gclrev390/+, tsl3/tsl4 and gclrev390/gclrev390, tsl3/tsl4 double mutants do not present any obvious defects in either centrosome positioning or division of primordial germ cells in the early embryo, as compared to controls.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (8)