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General Information
Symbol
Hsap\APLP2::Hsap\APPUAS.Tag:MYC
Species
H. sapiens
Name
FlyBase ID
FBal0095697
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

A chimeric construct in which the βA4 region and entire C-terminus of Hsap\APP695 has been replaced with the homologous region of Hsap\APLP2, tagged in the N-terminal region with Tag:MYC, is expressed under the control of UASt regulatory sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Expression of Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC induces cell death in the central nervous system (under the control of Scer\GAL4elav-C155), the developing eye (Scer\GAL4GMR.PF), thorax (Scer\GAL4pnr-MD237), and wing (Scer\GAL4ptc-559.1).

When Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC is driven by Scer\GAL4sca-537.4, a phenotype in the mechanosensory organs is seen.

When Hsap\APP695-Swedish.Scer\UAS is driven by Scer\GAL4Appl.G1a or Scer\GAL4179Y an accumulation of organelles is seen in larval axons (about 10 clogs per 20μm).

Flies expressing Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Mz1087.hx have a wing blister phenotype and develop a deformation in one or both wings. Large blisters may occupy more than 50% of the wing surface and in extreme cases the wings appear like balloons. The blisters appear transparent or black, are filled with hemolymph and occur in 5-20% of flies at 25oC. The pattern of wing veins is normal. Flies expressing Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC under the control of Scer\GAL469B or Scer\GAL4T80 have a wing blister phenotype. Flies expressing Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC under the control of Scer\GAL430A do not have a wing blister phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4Appl.G1a) is suppressed by the addition of Df(3L)GN24. The organelle clogging phenotype seen in mutant larvae (Driven by Scer\GAL4Appl.G1a) is suppressed by the addition of roblK/+.

Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Carried in a plasmid and transfected into SL-2 cells with a Scer\GAL4 expression plasmid. The expression and metabolism of Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC protein in these cells has been studied.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Symbol Synonym
Hsap\APLP2::Hsap\APPScer\UAS.T:Hsap\MYC
Hsap\APLP2::Hsap\APPT:Hsap\MYC
Hsap\APLP2::Hsap\APPT:Myc
Hsap\APLP2::Hsap\APPUAS.Tag:MYC
Name Synonyms
Secondary FlyBase IDs
    References (4)