FlyBase Allele Report: Dmel\pros[UAS.cMa]

General Information

Symbol

Dmel\pros^UAS.cMa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Matsuzaki

FlyBase ID

FBal0096724

Feature type

allele

Associated gene

Dmel\pros

Associated Insertion(s)

Carried in Construct

P{UAS-pros.17K-2}

Also Known As

UAS-pros

Key Links

Transgenic product class

wild_type

(Manning and Doe, 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Manning and Doe, 1999)

Mutagen

in vitro construct

(Manning and Doe, 1999, Reddy and Rodrigues, 1999)

Progenitor genotype

Carried in construct

P{UAS-pros.17K-2}

Cytology

Description

UAS regulatory sequences drive expression of full length pros.

(Manning and Doe, 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

pros

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates brain cancer

modeled by brat¹¹

(Bello et al., 2006)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

antenna, with Scer\GAL4^sca-P309

(Sen et al., 2003)

embryonic epidermis, with Scer\GAL4^Kr.PM

(Li and Vaessin, 2000)

eye disc morphogenetic furrow, with Scer\GAL4^dpp.blk1

(Kumar and Moses, 2001)

intermediate neuronal progenitor | larval stage, with Scer\GAL4^GMR9D11

(Bayraktar et al., 2010)

intermediate neuronal progenitor | larval stage, with Scer\GAL4^GMR19H09

(Bayraktar et al., 2010)

interommatidial bristle, with Scer\GAL4^sca-109-68

(Manning and Doe, 1999)

interommatidial bristle & tormogen cell, with Scer\GAL4^sca-109-68

(Manning and Doe, 1999)

interommatidial bristle & trichogen cell, with Scer\GAL4^sca-109-68

(Manning and Doe, 1999)

larval brain, with Scer\GAL4^hs.PB

(Colonques et al., 2011)

larval outer optic anlage, with Scer\GAL4^hs.PB

(Colonques et al., 2011)

larval ventral nerve cord, with Scer\GAL4^Kr.PM

(Li and Vaessin, 2000)

mesoderm, with Scer\GAL4^Kr.PM

(Li and Vaessin, 2000)

microchaeta & antennal segment 3, with Scer\GAL4^sca-P309

(Sen et al., 2003)

neuroblast | larval stage, with Scer\GAL4^wor.PA

(Cabernard and Doe, 2009)

neuroblast NB7-3, with Scer\GAL4^en-e16E

(Mettler et al., 2006)

neuron | larval stage, with Scer\GAL4^GMR9D11

(Bayraktar et al., 2010)

neuron | larval stage, with Scer\GAL4^GMR19H09

(Bayraktar et al., 2010)

scutum & external sensory organ & tormogen cell, with Scer\GAL4^sca-P309

(Reddy and Rodrigues, 1999)

scutum & external sensory organ & trichogen cell, with Scer\GAL4^sca-P309

(Reddy and Rodrigues, 1999)

scutum & macrochaeta, with Scer\GAL4^sca-109-68

(Manning and Doe, 1999)

scutum & macrochaeta & tormogen cell, with Scer\GAL4^sca-109-68

(Manning and Doe, 1999)

scutum & macrochaeta & trichogen cell, with Scer\GAL4^sca-109-68

(Manning and Doe, 1999)

scutum & microchaeta, with Scer\GAL4^sca-109-68

(Manning and Doe, 1999)

scutum & microchaeta & tormogen cell, with Scer\GAL4^sca-109-68

(Manning and Doe, 1999)

scutum & microchaeta & trichogen cell, with Scer\GAL4^sca-109-68

(Manning and Doe, 1999)

type II neuroblast | larval stage, with Scer\GAL4^GMR19H09

(Bayraktar et al., 2010)

Detailed Description

Statement

Reference

Larvae expressing pros^Scer\UAS.cMa under the control of pros^Scer\UAS.cMa (using heat shock) show a marked decrease in the number of mitotically active cells in the brain compared to controls. This reduction is seen both in the outer proliferation centre and in the central brain.

(Colonques et al., 2011)

Expression of pros^UAS.cMa under the control of Scer\GAL4^GMR19H09 does not cause type II to type I neuroblast transformation, but does cause loss of type II neuroblasts and reduction in the number of intermediate progenitors and neurons generated by type II neuroblasts at 120 hours after larval hatching. Expression of pros^UAS.cMa under the control of Scer\GAL4^GMR9D11 does not cause loss of type II neuroblasts, but does cause a large reduction in the number of intermediate progenitors and neurons generated by type II neuroblasts at 96 hours and 120 hours after larval hatching.

(Bayraktar et al., 2010)

Expression of pros^Scer\UAS.cMa in the neuroblasts under the control of Scer\GAL4^wor.PA results in depletion of larval neuroblast numbers.

(Cabernard and Doe, 2009)

Ectopic expression of pros^Scer\UAS.cMa, under the control of Scer\GAL4^en-e16E causes a precocious stop in cell division within the NB7-3 lineage.

(Mettler et al., 2006)

When pros^Scer\UAS.cMa is driven by Scer\GAL4^sca-P309, a striking reduction in external structures on the adult antenna is seen. No increase in the number of glia or neurons is seen.

(Sen et al., 2003)

Expression of pros^Scer\UAS.cMa under the control of Scer\GAL4^dpp.blk1 inhibits furrow reincarnation in the eye disc.

(Kumar and Moses, 2001)

When pros^Scer\UAS.cMa is driven by Scer\GAL4^eg-Mz360 or Scer\GAL4^sca-4512 no increase in NGB6-4t-derived glial cells is seen.

(Ragone et al., 2001)

Expression of pros^Scer\UAS.cMa under the control of Scer\GAL4^Kr.PM in embryos results in thinning and disruptions in the ventral nerve cord, and reduced epidermal and mesodermal structures. There is a strong reduction in BrdU labeling in these embryos compared to wild type.

(Li and Vaessin, 2000)

Animals expressing pros^Scer\UAS.cMa under the control of Scer\GAL4^sca-109-68 die as pharate adults and are virtually bald, having less than 1% of the normal external sense organ bristles in the notum and eye. The external sense organs lack both the bristle and socket.

(Manning and Doe, 1999)

Flies expressing pros^Scer\UAS.cMa under the control of Scer\GAL4^sca-P309 have a bald notum where both the shaft and socket of the bristles are absent.

(Reddy and Rodrigues, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^{elav.Switch.PO}, pros^UAS.cMa has abnormal cell cycle | larval stage phenotype, suppressible by Scer\GAL4^{elav.Switch.PO}, pros^UAS.cMa

(Kurusu et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/pros^UAS.cMa is a suppressor of abnormal neuroanatomy | somatic clone | adult stage phenotype of mir-279^S096207

(Hartl et al., 2011)

Scer\GAL4^{elav.Switch.PO}/pros^UAS.cMa is a suppressor of abnormal cell cycle | larval stage phenotype of Scer\GAL4^{elav.Switch.PO}, tll^UAS.cKa

(Kurusu et al., 2009)

Scer\GAL4^{elav.Switch.PO}, pros^UAS.cMa is a suppressor of abnormal cell cycle | larval stage phenotype of Scer\GAL4^{elav.Switch.PO}, pros^UAS.cMa

(Kurusu et al., 2009)

pros^UAS.cMa/Scer\GAL4^αTub84B.PL is a suppressor of abnormal neuroanatomy | somatic clone phenotype of brat¹¹

(Bello et al., 2006)

pros^UAS.cMa/Scer\GAL4^αTub84B.PL is a suppressor of increased cell number | somatic clone | larval stage phenotype of brat¹¹

(Bello et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^{elav.Switch.PO}, pros^UAS.cMa has brain | larval stage phenotype, suppressible by Scer\GAL4^{elav.Switch.PO}, pros^UAS.cMa

(Kurusu et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^Act.PU/pros^UAS.cMa is a suppressor of carbon dioxide sensitive neuron | ectopic | somatic clone phenotype of mir-279^S096207

(Hartl et al., 2011)

Scer\GAL4^wor.PA/pros^UAS.cMa is a suppressor of neuroblast | increased number phenotype of mud³/mud⁴

(Cabernard and Doe, 2009)

Scer\GAL4^{elav.Switch.PO}/pros^UAS.cMa is a suppressor of brain | larval stage phenotype of Scer\GAL4^{elav.Switch.PO}, tll^UAS.cKa

(Kurusu et al., 2009)

Scer\GAL4^{elav.Switch.PO}, pros^UAS.cMa is a suppressor of brain | larval stage phenotype of Scer\GAL4^{elav.Switch.PO}, pros^UAS.cMa

(Kurusu et al., 2009)

pros^UAS.cMa/Scer\GAL4^αTub84B.PL is a suppressor of larval brain | somatic clone phenotype of brat¹¹

(Bello et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of pros^Scer\UAS.cMa under the control of Scer\GAL4^Act.PU suppresses the formation of ectopic CO[[2]] neurons on the maxillary palp caused by mir-279^S096207 clones in 37% of animals.

(Hartl et al., 2011)

Co-overexpression of pros^Scer\UAS.cMa and tll^Scer\UAS.cKa by Scer\GAL4^{elav.Switch.PO} rescues the tll^Scer\UAS.cKa-induced brain hyperplasia in third instar larvae.

(Kurusu et al., 2009)

Expression of pros^Scer\UAS.cMa, under the control of Scer\GAL4^αTub84B.PL, in brat¹¹ mutant larval clones significantly suppresses the overproliferation phenotype in these clones in the central larval brain. Over 90% of Scer\GAL4^αTub84B.PL>pros^Scer\UAS.cMa, brat¹¹ clones in the central brain have <100 cells, while over 80% of brat¹¹ clones have 200-1000 cells.

(Bello et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^Act.PU/pros^UAS.cMa rescues pros^IG2227

(Hartl et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

F. Matsuzaki

(Manning and Doe, 1999)

F. Matsukazi

(Reddy and Rodrigues, 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

pros^Scer\UAS.cMa

pros^UAS.cMa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Matsuzaki

(Manning and Doe, 1999)

Secondary FlyBase IDs

References (19)

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