FlyBase Allele Report: Dmel\Dfd[UAS.cBa]

General Information

Symbol

Dmel\Dfd^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Brown

FlyBase ID

FBal0098520

Feature type

allele

Associated gene

Dmel\Dfd

Associated Insertion(s)

Carried in Construct

P{UAS-Dfd.B}

Also Known As

UAS-Dfd, UAS-Dfd^W4

Key Links

Transgenic product class

cDNA

(Brown et al., 1999)

wild_type

(Brown et al., 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Brown et al., 1999)

wild_type

(Brown et al., 1999)

Mutagen

in vitro construct

(Miller et al., 2001, Brown et al., 1999)

Progenitor genotype

Carried in construct

P{UAS-Dfd.B}

Cytology

Description

UASt regulatory sequences drive expression of the Dfd coding region (from the pC41 cDNA).

(Brown et al., 1999)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Dfd

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal, with Scer\GAL4^69B

(Miller et al., 2001)

lethal, with Scer\GAL4^{l(3)31-1-31-1}

(Miller et al., 2001)

lethal, with Scer\GAL4^prd.RG1

(Miller et al., 2001)

lethal - all die during embryonic stage, with Scer\GAL4^69B

(Brown et al., 1999)

some die during embryonic stage, with Scer\GAL4^69B

(Brown et al., 1999)

Phenotype Manifest In

abdominal lateral monoscolopidial chordotonal organ lch1 | embryonic stage, with Scer\GAL4^prd.RG1

(Robertson et al., 2004)

abdominal segment & embryonic/first instar larval cuticle, with Scer\GAL4^prd.RG1

(Robertson et al., 2004)

abdominal ventral monoscolopidial chordotonal organ vch1 | embryonic stage, with Scer\GAL4^prd.RG1

(Robertson et al., 2004)

cephalopharyngeal skeleton | posterior, with Scer\GAL4^69B

(Brown et al., 1999)

cirri | ectopic | embryonic stage, with Scer\GAL4^prd.RG1

(Robertson et al., 2004)

dorsal pharyngeal sensillum | embryonic stage, with Scer\GAL4^prd.RG1

(Robertson et al., 2004)

dorsal ridge, with Scer\GAL4^prd.RG1

(Robertson et al., 2004)

embryonic/larval oenocyte precursor, with Scer\GAL4^prd.RG1

(Robertson et al., 2004)

embryonic gastric caecum & parasegment 3, with Scer\GAL4^how-24B

(Miller et al., 2001)

embryonic head, with Scer\GAL4^69B

(Brown et al., 1999)

embryonic thoracic segment & embryonic/first instar larval cuticle, with Scer\GAL4^prd.RG1

(Robertson et al., 2004)

embryonic tritocerebrum, with Scer\GAL4^sca-537.4

(Sprecher et al., 2004)

larval intersegmental nerve, with Scer\GAL4^prd.RG1

(Robertson et al., 2004)

larval segmental nerve, with Scer\GAL4^prd.RG1

(Robertson et al., 2004)

presumptive embryonic/larval tracheal system, with Scer\GAL4^prd.RG1

(Robertson et al., 2004)

Detailed Description

Statement

Reference

Dfd^Scer\UAS.cBa; Scer\GAL4^prd.RG1 embryos form ectopic maxillary structures (rostral hairs and, occasionally, sclerotized rod-like structures) in the trunk cuticle. These ectopic structures develop near the Keilin's Organ primordia in the thoracic segments and in analogous positions in the abdominal segments. In disco^M.Scer\UAS; Scer\GAL4^prd.RG1 embryos the segments expressing disco do not complete dorsal closure. The affected segments are curved and resemble the gnathal lobes. In addition, the dorsal ridge is reduced in these embryos. In stage 13 disco^M.Scer\UAS; Scer\GAL4^prd.RG1 embryos, dorsal tracheal cells and oenocytes are missing in the segments ectopically expressing disco. The trunk sensilla and and associated neurons are remodeled in these embryos: chordotonal organs are absent, dorsal and ventral sensory organ groups are shifted and segmental/intersegmental neurons do not extend as far dorsally as in wild-type.

(Robertson et al., 2004)

Expression of Dfd^Scer\UAS.cBa under the control of Scer\GAL4^sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 70-80%.

(Sprecher et al., 2004)

Expression of Dfd^Scer\UAS.cBa under the control of Scer\GAL4^lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain.

(Hirth et al., 2001)

When Dfd^Scer\UAS.cBa is driven by Scer\GAL4^how-24B, no homeosis in the abdomen is seen. These animals show reduced gastric caecum development at parasegment 3.

(Miller et al., 2001)

When driven by Scer\GAL4^69B, Scer\GAL4^prd.RG1, or Scer\GAL4^{l(3)31-1-31-1} leads to a significant loss in viability.

(Miller et al., 2001)

Head involution fails in Dfd¹²/Df(3R)Scr embryos expressing Dfd^Scer\UAS.cBa under the control of Scer\GAL4^69B and the posterior mouthparts fail to form. These embryos die without hatching.

(Brown et al., 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Dfd^UAS.cBa, Scer\GAL4^GMR.long is a non-suppressor of visible | adult stage phenotype of Scer\GAL4^GMR.long, Zzzz\GGGGCC^30.UAS.EGFP

(Jiao et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

Dfd^UAS.cBa, Scer\GAL4^prd.RG1, tsh⁸ has embryonic abdominal segment phenotype, enhanceable by disco^M.UAS, Scer\GAL4^prd.RG1

(Robertson et al., 2004)

Dfd^UAS.cBa, Scer\GAL4^prd.RG1, tsh⁸ has embryonic thoracic segment phenotype, enhanceable by disco^M.UAS, Scer\GAL4^prd.RG1

(Robertson et al., 2004)

Dfd^UAS.cBa, Scer\GAL4^prd.RG1, tsh⁸ has cirri | ectopic phenotype, enhanceable by disco^M.UAS, Scer\GAL4^prd.RG1

(Robertson et al., 2004)

Dfd^UAS.cBa, Scer\GAL4^prd.RG1 has embryonic abdominal segment phenotype, enhanceable by tsh⁸

(Robertson et al., 2004)

Dfd^UAS.cBa, Scer\GAL4^prd.RG1 has embryonic thoracic segment phenotype, enhanceable by tsh⁸

(Robertson et al., 2004)

Dfd^UAS.cBa, Scer\GAL4^prd.RG1 has cirri | ectopic phenotype, enhanceable by tsh⁸

(Robertson et al., 2004)

Suppressed by

Statement

Reference

Dfd^UAS.cBa, Scer\GAL4^prd.RG1 has abdominal segment & embryonic/first instar larval cuticle phenotype, suppressible by Df(1)XR14

(Robertson et al., 2004)

Dfd^UAS.cBa, Scer\GAL4^prd.RG1 has embryonic thoracic segment & embryonic/first instar larval cuticle phenotype, suppressible by Df(1)XR14

(Robertson et al., 2004)

Dfd^UAS.cBa, Scer\GAL4^prd.RG1 has cirri | ectopic | embryonic stage phenotype, suppressible by Df(1)XR14

(Robertson et al., 2004)

Suppressor of

Statement

Reference

Scer\GAL4^lab.PH/Dfd^UAS.cBa is a suppressor of embryonic tritocerebrum phenotype of lab¹⁴

(Hirth et al., 2001)

NOT Suppressor of

Statement

Reference

Dfd^UAS.cBa, Scer\GAL4^GMR.long is a non-suppressor of eye phenotype of Scer\GAL4^GMR.long, Zzzz\GGGGCC^30.UAS.EGFP

(Jiao et al., 2021)

Other

Statement

Reference

Dfd¹⁶, Dfd^UAS.cBa, Scer\GAL4^salm-459.2, Scr⁴ has adult corpus allatum phenotype

(Sánchez-Higueras et al., 2014)

Dfd¹⁶, Dfd^UAS.cBa, Scer\GAL4^salm-459.2, Scr⁴ has prothoracic gland phenotype

(Sánchez-Higueras et al., 2014)

Dfd^UAS.cBa, Scer\GAL4^prd.RG1, tsh⁸ has mouth hook | ectopic phenotype

(Robertson et al., 2004)

Dfd^UAS.cBa, Scer\GAL4^arm.PS, disco^M.UAS, tsh⁸ has mouth hook | ectopic phenotype

(Robertson et al., 2004)

Dfd^UAS.cBa, Scer\GAL4^prd.RG1, disco^M.UAS, tsh⁸ has mouth hook | ectopic phenotype

(Robertson et al., 2004)

Additional Comments

Genetic Interactions

Statement

Reference

Addition of Dfd^Scer\UAS.cBa to Dfd¹⁶;Scr⁴ double mutant embryos (under the control of Scer\GAL4^salm-459.2) results in both the Mx and Lb segments forming corpora allata.

(Sánchez-Higueras et al., 2014)

Formation of ectopic maxillary structures in trunk segments of Dfd^Scer\UAS.cBa; Scer\GAL4^prd.RG1 embryos is suppressed by Df(1)XR14/Y.

tsh⁸/tsh⁸ enhances the formation of ectopic maxillary structures in thoracic and abdominal segments of Dfd^Scer\UAS.cBa; Scer\GAL4^prd.RG1 embryos: ectopic mouthpart-like material and rostral hairs are present in each affected segment, with well-shaped mouth hooks frequently observed, though the ectopic sclerotized material also can lack a specific shape. When disco^M.Scer\UAS is also present the enhancement is even stronger: Ectopic mouth hooks and rostral hairs form in almost all expressing segments, and amorphous sclerotized material is not seen. When Scer\GAL4^arm.PS is substituted as a driver in this experiment, ectopic mouth-hooks form in all segments.

Formation of ectopic maxillary structures in trunk segments of Dfd^Scer\UAS.cBa; Scer\GAL4^prd.RG1 embryos is suppressed by Df(1)XR14/Y. tsh⁸/tsh⁸ enhances the formation of ectopic maxillary structures in thoracic and abdominal segements of Dfd^Scer\UAS.cBa; Scer\GAL4^prd.RG1 embryos: ectopic mouthpart-like material and rostral hairs are present in each affected segment, with well-shaped mouth hooks frequently observed, though the ectopic sclerotized material also can lack a specific shape. When disco^M.Scer\UAS is also present the enhancement is even stronger: Ectopic mouth hooks and rostral hairs form in almost all expressing segments, and amorphous sclerotized material is not seen. When Scer\GAL4^arm.PS is substituted as a driver in this experiment, ectopic mouth-hooks form in all segments.

(Robertson et al., 2004)

Expression of Dfd^Scer\UAS.cBa under the control of Scer\GAL4^lab.PH rescues the tritocerebral defects seen in lab¹⁴ embryonic brains. 38.9% of embryos show a complete rescue of the defects (taking into account that the phenotypic penetrance of the lab¹⁴ phenotype is 88.6%).

(Hirth et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Dfd^UAS.cBa/Scer\GAL4^69B partially rescues Dfd¹²/Df(3R)Scr

(Brown et al., 1999)

Comments

The mouthhook and cirri defects of Dfd¹²/Df(3R)Scr embryos are rescued by expression of Dfd^Scer\UAS.cBa under the control of Scer\GAL4^69B.

(Brown et al., 1999)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7299

w¹¹¹⁸; P{UAS-Dfd.B}W4

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Dfd^Scer\UAS.cBa

Dfd^Scer\UAS.cMa

Dfd^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Brown

(Brown et al., 1999)

Secondary FlyBase IDs

FBal0124324

References (12)

Report Sections

Open Close