Rab6^D23D germline clones show oocyte-nurse cell membranes and membranes between nurse cells starting to collapse after stage 3.

Rab6^D23D eye clones made using the 'eyFLP' system display no "on" and "off" transients in electroretinograms but exhibit a normal depolarization. There are no differentiation defects in these eyes.

18.8% of R7 cells fail to target to the correct layers in adult brains containing Rab6^D23D clones made using the 'eyFLP' system.

In Rab6^D23D/+ females that carry Rab6^D23D germline clones, cyst formation and maturation appear normal. However, from stages 2 to 7, over 80% of Rab6^D23D egg chambers display a progressive disappearance of the nurse cell cortical actin cytoskeleton, aberrantly spaced nurse cell nuclei and a concomitant aggregation of ring canals and actin debris in the egg chamber. The phenotype falls into two broad classes: 32.5% of Rab6^D23D egg chambers have a severe phenotype in which all cortical actin is absent. Such egg chambers continue to grow but ultimately degenerate and do not develop beyond stage 7. The second class (67.5%) show a more heterogeneous phenotype in which all egg chambers possess a delimited oocyte.

Egg chambers from Rab6^D23D germline clones form 'open' syncytia instead of forming the compartmentalized cysts seen in wild-type chambers. In strongly affected rab6D23D egg chambers, all nuclei are found within a single open syncytium, with nuclei at the periphery of a common cytoplasm that has a mass of membranes, ring canals and actin debris at its center. Rab6^D23D egg chambers that show membrane loss but manage to develop past stage 7 display a stereotypic organization at mid-oogenesis. In these chambers, 12 out of the 15 nurse cell nuclei were reproducibly contained within two open syncytia, the first comprising eight, and the second four, nuclei. Oocyte fate is not affected and neither is the position of the nucleus within the oocyte.

Rab6^D23D egg chambers show an enlargement of the Golgi, but not of the ER compartment. Additionally, nearly 70% show mispolarization of the microtubule network.