FlyBase Allele Report: Dmel\p53[Ct.GUS]

General Information

Symbol

Dmel\p53^Ct.GUS

Species

D. melanogaster

Name

FlyBase ID

FBal0104952

Feature type

allele

Associated gene

Dmel\p53

Associated Insertion(s)

Carried in Construct

P{GUS-p53.Ct}

Key Links

Transgenic product class

dominant_negative_variant

(Brodsky et al., 2000)

inframe_deletion

(Brodsky et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Brodsky et al., 2000)

inframe_deletion

(Brodsky et al., 2000)

Mutagen

in vitro construct

(Brodsky et al., 2000)

Progenitor genotype

Carried in construct

P{GUS-p53.Ct}

Cytology

Description

Encodes the C-terminal fragment of p53 with defective DNA binding activity.

(Lee et al., 2003)

UAS and GMR regulatory sequences are fused of a C-terminal fragment (amino acids 282 to 385) of p53.

(Brodsky et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

UAS

binary expression system - regulatory region

Encoded product / tool

p53

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^{Arctic.Aβ42.UAS.Tag:SS(rPENK)}

(Iijima et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

chemical resistant | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Bauer et al., 2005)

decreased cell death, with Scer\GAL4^en-e16E

(Brodsky et al., 2000)

decreased cell death, with Scer\GAL4^GMR.PF

(Lee et al., 2006)

long lived, with Scer\GAL4^elav-C155

(Bauer et al., 2005)

long lived | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Bauer et al., 2005)

Phenotype Manifest In

wing disc posterior compartment, with Scer\GAL4^en-e16E

(Brodsky et al., 2000)

Detailed Description

Statement

Reference

Expression of p53^Ct.GUS under the control of Scer\GAL4^Bx-MS1096 has no effect on wing development.

(Villicaña et al., 2013)

Expression of the dominant-negative p53^Ct.GUS is expressed by Scer\GAL4^GMR.PF suppresses ionizing radiation-dependent apoptosis in the ninaE-expressing region.

(Lee et al., 2006)

Expression of p53^Ct.GUS, under the control of Scer\GAL4^elav-C155, extends lifespan by 58% in females and 32% in males. Expression of p53^Ct.GUS in adult neurons, driven by Scer\GAL4^{elav.Switch.PO} after induction with RU486, leads to a 18-26% increase in maximum lifespan in females fed on standard cornmeal food. When flies are fed on a high calorie diet, the lifespan of both males and females of this genotype is extended (by 13 and 11%, respectively). However, the lifespan of Scer\GAL4^{elav.Switch.PO}>p53^Ct.GUS flies is not increased further than the increase seen in controls when flies are fed on a low calorie diet.

The expression of p53^Ct.GUS in the muscle, driven by Scer\GAL4^{Mhc.Switch.PO}, or in the fat body, driven by either Scer\GAL4^Switch1.106 or Scer\GAL4^{bun-Switch1.32}, does not extend the lifespan of flies.

Flies that express p53^Ct.GUS, under the control of Scer\GAL4^{elav.Switch.PO}, are more resistant to paraquat poisoning than control flies. However, these flies are no more resistant to starvation or heat stress than controls. The daily egg production is not statistically different in Scer\GAL4^{elav.Switch.PO}>p53^Ct.GUS females from controls. There is also no difference in the total or average amount of spontaneous activity between Scer\GAL4^{elav.Switch.PO}>p53^Ct.GUS flies and control flies.

(Bauer et al., 2005)

Co-expression of p53^Ct.GUS suppresses the disruption of the eye caused by expression of p53^EP-1 under the control of Scer\GAL4^GMR.PF.

(Lee et al., 2003)

When expression is driven by Scer\GAL4^en-e16E in the posterior compartment of the wing disc radiation-induced cell death is dramatically reduced, compared to that in the anterior compartment. There is no effect on the block of entry into mitosis upon irradiation, which occurs as it does for wild type discs. There is also no notable effect on wing pattern and size.

(Brodsky et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, mrn^GD4089, p53^Ct.GUS has increased cell death phenotype, suppressible by bsk^GD10555/Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

Scer\GAL4^Bx-MS1096, mrn^GD4089, p53^Ct.GUS has increased cell death phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

Enhancer of

Statement

Reference

p53^Ct.GUS/Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of Scer\GAL4^Bx-MS1096, mrn^GD4089

(Villicaña et al., 2013)

p53^Ct.GUS/Scer\GAL4^Bx-MS1096 is an enhancer of increased cell death phenotype of Scer\GAL4^Bx-MS1096, mrn^GD4089

(Villicaña et al., 2013)

NOT Suppressor of

Statement

Reference

p53^Ct.GUS/Scer\GAL4^GMR.PU is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PU, Sirt1^UAS.cGa

(Griswold et al., 2008)

p53^Ct.GUS/Scer\GAL4^GMR.PU is a non-suppressor of abnormal eye color phenotype of Scer\GAL4^GMR.PU, Sirt1^UAS.cGa

(Griswold et al., 2008)

p53^Ct.GUS, Scer\GAL4^GMR.PF is a non-suppressor of increased cell death phenotype of Lkb1^UAS.cLa, Scer\GAL4^GMR.PF

(Lee et al., 2006)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, mrn^GD4089, p53^Ct.GUS has wing disc phenotype, suppressible by BacA\p35^UAS.cUa, Scer\GAL4^Bx-MS1096

(Villicaña et al., 2013)

Enhancer of

Statement

Reference

p53^Ct.GUS/Scer\GAL4^Bx-MS1096 is an enhancer of wing disc phenotype of Scer\GAL4^Bx-MS1096, mrn^GD4089

(Villicaña et al., 2013)

p53^Ct.GUS/Scer\GAL4^Bx-MS1096 is an enhancer of wing phenotype of Scer\GAL4^Bx-MS1096, mrn^GD4089

(Villicaña et al., 2013)

NOT Suppressor of

Statement

Reference

p53^Ct.GUS/Scer\GAL4^GMR.PU is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PU, Sirt1^UAS.cGa

(Griswold et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of p53^Ct.GUS enhances the wing defects seen in flies expressing mrn^GD4089 under the control of Scer\GAL4^Bx-MS1096.

Co-expression of p53^Ct.GUS enhances the increased apoptosis seen in the wing discs of animals expressing mrn^GD4089 under the control of Scer\GAL4^Bx-MS1096.

The massive apoptosis seen in wing discs co-expressing p53^Ct.GUS and mrn^GD4089 under the control of Scer\GAL4^Bx-MS1096 is practically abolished by co-expression of bsk^GD10555.

(Villicaña et al., 2013)

Co-expression of p53^Ct.GUS does not suppress the defective eye phenotype caused by expression of Sir2^Scer\UAS.cGa under the control of Scer\GAL4^GMR.PU.

(Griswold et al., 2008)

Xenogenetic Interactions

Statement

Reference

The massive apoptosis seen in wing discs co-expressing p53^Ct.GUS and mrn^GD4089 under the control of Scer\GAL4^Bx-MS1096 is suppressed by co-expression of BacA\p35^Scer\UAS.cUa.

(Villicaña et al., 2013)

Co-expression of p53^Ct.GUS does not rescue the autonomous reduction in wing area caused by expression of Rcom\RA^cs2.Scer\UAS under the control of Scer\GAL4^en-e16E, but it does rescue the non-autonomous reduction in wing area caused by expression of Rcom\RA^cs2.Scer\UAS under the control of Scer\GAL4^en-e16E.

(Mesquita et al., 2010)

lkb1^Scer\UAS.cLa-induced apoptosis is not suppressed by expression of the dominant-negative p53^Ct.GUS, both under the control of Scer\GAL4^GMR.PF.

(Lee et al., 2006)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6583

w¹¹¹⁸; P{GUS-p53.Ct}3.1/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Carried in a plasmid, transfected into S2 cells and used in transcription activation assays. Dominant negative mutation.

(Brodsky et al., 2000)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

dp53^CT

(Mesquita et al., 2010)

dp53^DN

(Mesquita et al., 2010)

p53^Ct.GUS

p53^DN

(Flegel et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (18)

Report Sections

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