FlyBase Allele Report: Dmel\Tak1[K46R.UAS]

General Information

Symbol

Dmel\Tak1^K46R.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0117636

Feature type

allele

Associated gene

Dmel\Tak1

Associated Insertion(s)

Carried in Construct

P{UAS-Tak1.K46R}

Also Known As

UAS-dTak1^DN

Key Links

Transgenic product class

dominant_negative_variant

(Takatsu et al., 2000)

missense_variant

(Takatsu et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

dominant_negative_variant

(Takatsu et al., 2000)

missense_variant

(Takatsu et al., 2000)

Mutagen

in vitro construct

(Takatsu et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Tak1.K46R}

Cytology

Description

A dominant negative Tak1 cDNA (carries the amino acid replacement K46R) is expressed under the control of UASt sequences.

(Takatsu et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Tak1

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Ras85D^V12.UAS, fmt¹

(Ma et al., 2017)

modeled by Ras85D^V12.UAS, PpV^Δ1

(Ma et al., 2017)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased cell death | embryonic stage, with Scer\GAL4^Act5C.PU

(Yang and Su, 2011)

decreased cell death | embryonic stage, with Scer\GAL4^how-24B

(Yang and Su, 2011)

visible, with Scer\GAL4^pnr-MD237

(Takatsu et al., 2000)

Phenotype Manifest In

embryo, with Scer\GAL4^pnr-MD237

(Takatsu et al., 2000)

embryo | anterior, with Scer\GAL4^pnr-MD237

(Takatsu et al., 2000)

embryonic/first instar larval cuticle | dorsal, with Scer\GAL4^pnr-MD237

(Takatsu et al., 2000)

embryonic head, with Scer\GAL4^pnr-MD237

(Takatsu et al., 2000)

eye, with Scer\GAL4^GMR.PF

(Takatsu et al., 2000)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Takatsu et al., 2000)

mouth hook, with Scer\GAL4^pnr-MD237

(Takatsu et al., 2000)

secondary pigment cell, with Scer\GAL4^GMR.PF

(Takatsu et al., 2000)

tertiary pigment cell, with Scer\GAL4^GMR.PF

(Takatsu et al., 2000)

Detailed Description

Statement

Reference

Expression of Tak1^{K46R.Scer\UAS} under the control of Scer\GAL4^wor.PA does not induce neuroblast loss in larval brains.

(Kuang et al., 2014)

Tak1^{K46R.Scer\UAS} driven by Scer\GAL4^pnr-MD237 causes various cuticle defects in embryos. Head structure defects are seen in which mouth hooks are missing. Some of these defective embryos also exhibit an anterior open phenotype. In the most extreme cases the entire dorsal cuticle fail to close. Occasionally U shaped embryos are seen. Flies expressing Tak1^{K46R.Scer\UAS} driven by Scer\GAL4^GMR.PF display a rough eye phenotype in a dose dependant manner. In the pupal eye disc, bristles are mislocalised. The cell shapes of secondary and tertiary pigment cells are heterogeneous.

(Takatsu et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^Act5C.PU, Tak1^K46R.UAS has decreased cell death | embryonic stage phenotype, non-enhanceable by Scer\GAL4^Act5C.PU/dpp^UAS.cUa

(Yang and Su, 2011)

Scer\GAL4^how-24B, Tak1^K46R.UAS has decreased cell death | embryonic stage phenotype, non-enhanceable by raw¹

(Yang and Su, 2011)

NOT suppressed by

Statement

Reference

Scer\GAL4^Act5C.PU, Tak1^K46R.UAS has decreased cell death | embryonic stage phenotype, non-suppressible by Scer\GAL4^Act5C.PU/dpp^UAS.cUa

(Yang and Su, 2011)

Scer\GAL4^how-24B, Tak1^K46R.UAS has decreased cell death | embryonic stage phenotype, non-suppressible by raw¹

(Yang and Su, 2011)

Suppressor of

Statement

Reference

Tak1^K46R.UAS/Scer\GAL4^ptc.PU is a suppressor | partially of visible | adult stage phenotype of Scer\GAL4^ptc.PU, fmt^GD6928

(Ma et al., 2017)

Tak1^K46R.UAS, Scer\GAL4^FRT.Act5C is a suppressor | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, fmt¹

(Ma et al., 2017)

Tak1^K46R.UAS, Scer\GAL4^FRT.Act5C is a suppressor | somatic clone of neoplasia | somatic clone | third instar larval stage phenotype of PpV^Δ1, Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C

(Ma et al., 2017)

Scer\GAL4^Act5C.PU/Tak1^K46R.UAS is a suppressor of increased cell death | embryonic stage phenotype of Scer\GAL4^Act5C.PU, dpp^UAS.cUa

(Yang and Su, 2011)

Scer\GAL4^how-24B/Tak1^K46R.UAS is a suppressor of increased cell death | embryonic stage phenotype of raw¹

(Yang and Su, 2011)

Tak1^K46R.UAS/Scer\GAL4^69B is a suppressor of visible phenotype of Scer\GAL4^69B, nmo^c5-1.UAS

(Mirkovic et al., 2002)

NOT Suppressor of

Statement

Reference

Tak1^K46R.UAS/Scer\GAL4^GMR.PF is a non-suppressor of increased cell death phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

Tak1^K46R.UAS/Scer\GAL4^69B is an enhancer of wing vein | ectopic phenotype of Scer\GAL4^69B, nmo^c5-1.UAS

(Mirkovic et al., 2002)

Scer\GAL4^LE/Tak1^K46R.UAS is an enhancer of embryonic epidermis | dorsal phenotype of arm³

(McEwen et al., 2000)

Suppressor of

Statement

Reference

Tak1^K46R.UAS, Scer\GAL4^FRT.Act5C is a suppressor | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of PpV^Δ1, Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C

(Ma et al., 2017)

Tak1^K46R.UAS/Scer\GAL4^ptc.PU is a suppressor | partially of anterior crossvein phenotype of Scer\GAL4^ptc.PU, fmt^GD6928

(Ma et al., 2017)

Tak1^K46R.UAS, Scer\GAL4^FRT.Act5C is a suppressor | somatic clone of eye-antennal disc | somatic clone | third instar larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, fmt¹

(Ma et al., 2017)

Scer\GAL4^how-24B/Tak1^K46R.UAS is a suppressor of embryonic cardioblast phenotype of raw¹

(Yang and Su, 2011)

Scer\GAL4^how-24B/Tak1^K46R.UAS is a suppressor of embryonic pericardial cell phenotype of raw¹

(Yang and Su, 2011)

Scer\GAL4^GMR.PF/Tak1^K46R.UAS is a suppressor of eye phenotype of Scer\GAL4^GMR.PF, egr^GS9830

(Igaki et al., 2002)

Tak1^K46R.UAS/Scer\GAL4^69B is a suppressor of posterior crossvein phenotype of Scer\GAL4^69B, nmo^c5-1.UAS

(Mirkovic et al., 2002)

NOT Suppressor of

Statement

Reference

Tak1^K46R.UAS/Scer\GAL4^GMR.PF is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF, nopo^UAS.cUa

(Ma et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

The strong tumorigenic activity of Scer\GAL4^{Scer\FRT.Act5C}>Ras85D^V12.Scer\UAS;fmt¹/fmt¹ MARCM clones in third instar larval eye-antennal discs is strongly impeded and their invasive capacity abolished by co-expression of Tak1^{K46R.Scer\UAS} in the clones.

Similarly, the tumorigenicity of Ras85D^V12.Scer\UAS-expressing clones is also enhanced if the clones are mutant for PpV^Δ1, they display invasive behavior and increased mitotic activity; these characteristics are suppressed by co-expression of Tak1^{K46R.Scer\UAS}.

The partial or complete loss of the wing anterior crossvein in flies expressing fmt^GD6928 under the control of Scer\GAL4^ptc.PU is partially suppressed by co-expression of Tak1^{K46R.Scer\UAS}.

(Ma et al., 2017)

Co-expression of dominant-negative Tak1^{K46R.Scer\UAS} does not suppress the small, rough eye phenotype found upon expression of nopo^Scer\UAS.cUa under the control of Scer\GAL4^GMR.PF.

(Ma et al., 2012)

The addition of hep¹/+ to egr^GS9830, Scer\GAL4^GMR.PF animals leads to a suppression of the eye phenotype. The addition of hep¹/Y leads to an almost complete suppression of the eye phenotype.

(Igaki et al., 2002)

Tak1^{K46R.Scer\UAS} rescues the posterior cross vein, wing shape and size defects seen in Scer\GAL4^69B, nmo^{c5-1.Scer\UAS} adults, but enhances the L2 extra vein phenotype.

(Mirkovic et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tak1^{K46R.Scer\UAS}

Tak1^K46R.UAS

Name Synonyms

Secondary FlyBase IDs

References (9)

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