FlyBase Allele Report: Dmel\raw[1]

General Information

Symbol

Dmel\raw¹

Species

D. melanogaster

Name

FlyBase ID

FBal0014432

Feature type

allele

Associated gene

Dmel\raw

Associated Insertion(s)

Carried in Construct

Also Known As

raw^IG

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: ?609term.

(Byars et al., 1999)

Nucleotide substitution: C?T.

(Byars et al., 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:8,711,779..8,711,779 [-]

Nucleotide change:

C8711779T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

?610term

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement.

(Byars et al., 1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | embryonic stage

(Yang and Su, 2011)

increased cell death | embryonic stage

(Yang and Su, 2011)

Phenotype Manifest In

denticle

(Blake et al., 1999)

embryo | dorsal

(Yang and Su, 2011)

embryo | dorsal closure stage

(Blake et al., 1999)

embryonic/first instar larval cuticle | ventral

(Byars et al., 1999)

embryonic/larval hindgut | embryonic stage

(Blake et al., 1999, Blake et al., 1998)

embryonic/larval midgut | embryonic stage

(Jack and Myette, 1997)

embryonic/larval salivary gland | embryonic stage

(Blake et al., 1999, Blake et al., 1998)

embryonic cardioblast

(Yang and Su, 2011)

embryonic epidermis

(Byars et al., 1999, Blake et al., 1998)

embryonic epidermis | dorsal

(Jack and Myette, 1997)

embryonic head

(Byars et al., 1999)

embryonic midgut constriction

(Jack and Myette, 1997)

embryonic myoblast

(Yang and Su, 2011)

embryonic pericardial cell

(Yang and Su, 2011)

larval dorsal hair

(Blake et al., 1999)

Malpighian tubule

(Blake et al., 1999, Jack and Myette, 1997)

mesoderm | dorsal

(Yang and Su, 2011)

mitotic domain 1 | extended germ band stage

(Byars et al., 1999)

presumptive embryonic/larval central nervous system

(Jack and Myette, 1997)

presumptive embryonic/larval peripheral nervous system

(Byars et al., 1999)

ventral denticle belt | absent | embryonic stage

(Yang and Su, 2011)

Detailed Description

Statement

Reference

The salivary gland of mutant embryos is shorter and wider than wild type. Malpighian tubules are short and wide. The hindgut is distorted. The raw¹ Malpighian tubule phenotype is partially suppressed by zip^mhc-c1.3 or zip². The amnioserosa remains completely exposed in mutant embryos, due to a failure in dorsal closure. Cuticular hairs are generally disorganised in appearance in mutant embryos, may be inappropriately clustered and are often forked or branched.

(Blake et al., 1999)

Homozygous embryos have a dorsal epidermal hole, defects in head involution and germband retraction and poorly differentiated ventral cuticle. The ventral cuticle does not have dorsal hairs, indicating that it is not transformed to a dorsal fate. Wild-type patterns of asymmetric gastrulation are seen in these embryos. Elongated neuronal cell bodies, thickened axon bundles and fasciculation defects are seen in the peripheral nervous system. The amnioserosal membrane is readily apparent in germband-retracted, stage 13-14 homozygous embryos and the cells in the amnioserosa are not apoptotic. Elongated leading edge cells are seen in homozygous embryos undergoing dorsal closure.

(Byars et al., 1999)

Almost all epidermal cells remain polygonal during dorsal closure in homozygous embryos, in contrast to wild-type where the cells elongate dorsoventrally. The epidermis appears wrinkled and the dorsal edge is folded inward. The shape of the salivary glands and hindgut is abnormal (although the hindgut phenotype is variable and the hindgut often resembles the wild-type), and the cells of both these organs are less columnar than in wild-type; salivary gland cells are approximately the same width as wild-type, but shorter than wild-type, and hindgut cells are both wider and shorter than wild-type. The salivary gland cells lack the wedge shape characteristic of wild-type salivary gland cells.

(Blake et al., 1998)

Malpighian tubules have a greater circumference than normal at their proximal end in homozygous embryos, giving them a gourd-like shape. The lumen of the tubules is greatly enlarged compared to wild-type. The epidermis never closes dorsally, the central nervous system fails to retract and midgut constrictions are incomplete.

(Jack and Myette, 1997)

embryonic lethal dorsal closure and cuticle differentiation defective.

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

raw¹ has increased cell death | embryonic stage phenotype, suppressible by Scer\GAL4^how-24B/BacA\p35^UAS.cHa

(Yang and Su, 2011)

raw¹ has increased cell death | embryonic stage phenotype, suppressible by Scer\GAL4^how-24B/tkv^KK102319

(Yang and Su, 2011)

raw¹ has increased cell death | embryonic stage phenotype, suppressible by Scer\GAL4^how-24B/Tak1^K46R.UAS

(Yang and Su, 2011)

raw¹ has increased cell death | embryonic stage phenotype, suppressible by p53^DN.UAS.cUa/Scer\GAL4^how-24B

(Yang and Su, 2011)

NOT suppressed by

Statement

Reference

raw¹ has increased cell death | embryonic stage phenotype, non-suppressible by wg^UAS.cUa/Scer\GAL4^69B

(Yang and Su, 2011)

raw¹ has increased cell death | embryonic stage phenotype, non-suppressible by Scer\GAL4^how-24B/brk^UAS.cUa

(Yang and Su, 2011)

NOT Enhancer of

Statement

Reference

raw¹ is a non-enhancer of increased cell death | embryonic stage phenotype of tkv⁷

(Yang and Su, 2011)

raw¹ is a non-enhancer of decreased cell death | embryonic stage phenotype of Scer\GAL4^how-24B, Tak1^K46R.UAS

(Yang and Su, 2011)

Suppressor of

Statement

Reference

raw¹/raw[+] is a suppressor of partially lethal - majority die phenotype of slpr^BS06

(Polaski et al., 2006)

NOT Suppressor of

Statement

Reference

raw¹ is a non-suppressor of decreased cell death | embryonic stage phenotype of Scer\GAL4^how-24B, Tak1^K46R.UAS

(Yang and Su, 2011)

raw¹ is a non-suppressor of increased cell death | embryonic stage phenotype of tkv⁷

(Yang and Su, 2011)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

raw¹ has embryo | dorsal phenotype, non-enhanceable by tkv⁷

(Yang and Su, 2011)

Suppressed by

Statement

Reference

raw¹ has embryonic cardioblast phenotype, suppressible by Scer\GAL4^how-24B/tkv^KK102319

(Yang and Su, 2011)

raw¹ has embryonic cardioblast phenotype, suppressible by p53^DN.UAS.cUa/Scer\GAL4^how-24B

(Yang and Su, 2011)

raw¹ has embryonic pericardial cell phenotype, suppressible by Scer\GAL4^how-24B/tkv^KK102319

(Yang and Su, 2011)

raw¹ has embryonic cardioblast phenotype, suppressible by Scer\GAL4^how-24B/Tak1^K46R.UAS

(Yang and Su, 2011)

raw¹ has embryonic pericardial cell phenotype, suppressible by Scer\GAL4^how-24B/Tak1^K46R.UAS

(Yang and Su, 2011)

raw¹ has embryonic pericardial cell phenotype, suppressible by p53^DN.UAS.cUa/Scer\GAL4^how-24B

(Yang and Su, 2011)

raw¹ has embryonic/larval salivary gland | embryonic stage phenotype, suppressible by zip^mhc-c1.3

(Blake et al., 1999)

raw¹ has Malpighian tubule phenotype, suppressible by zip^mhc-c1.3

(Blake et al., 1999)

raw¹ has embryonic/larval salivary gland | embryonic stage phenotype, suppressible by zip²

(Blake et al., 1999)

raw¹ has Malpighian tubule phenotype, suppressible by zip²

(Blake et al., 1999)

raw¹ has embryo | dorsal closure stage phenotype, suppressible by zip²

(Blake et al., 1999)

raw¹ has denticle phenotype, suppressible by zip²

(Blake et al., 1999)

raw¹ has larval dorsal hair phenotype, suppressible by zip²

(Blake et al., 1999)

raw¹ has embryonic/first instar larval cuticle | ventral phenotype, suppressible by Jra^IA109

(Byars et al., 1999)

raw¹ has embryonic/first instar larval cuticle | ventral phenotype, suppressible by Scer\GAL4^69B/aop^ACT.UAS

(Byars et al., 1999)

NOT suppressed by

Statement

Reference

raw¹ has embryonic pericardial cell phenotype, non-suppressible by Scer\GAL4^how-24B/brk^UAS.cUa

(Yang and Su, 2011)

raw¹ has embryonic pericardial cell phenotype, non-suppressible by wg^UAS.cUa/Scer\GAL4^69B

(Yang and Su, 2011)

raw¹ has embryonic cardioblast phenotype, non-suppressible by wg^UAS.cUa/Scer\GAL4^69B

(Yang and Su, 2011)

raw¹ has embryonic cardioblast phenotype, non-suppressible by Scer\GAL4^how-24B/brk^UAS.cUa

(Yang and Su, 2011)

raw¹ has embryo | dorsal phenotype, non-suppressible by tkv⁷

(Yang and Su, 2011)

raw¹ has Malpighian tubule phenotype, non-suppressible by zip^mhc-c2.1

(Blake et al., 1999)

raw¹ has Malpighian tubule phenotype, non-suppressible by zip^mhc-c3.12

(Blake et al., 1999)

raw¹ has Malpighian tubule phenotype, non-suppressible by zip^mhc-c6.1

(Blake et al., 1999)

raw¹ has embryonic/larval salivary gland | embryonic stage phenotype, non-suppressible by zip^mhc-c2.1

(Blake et al., 1999)

raw¹ has embryonic/larval salivary gland | embryonic stage phenotype, non-suppressible by zip^mhc-c3.12

(Blake et al., 1999)

raw¹ has embryonic/larval salivary gland | embryonic stage phenotype, non-suppressible by zip^mhc-c6.1

(Blake et al., 1999)

Other

Statement

Reference

raw¹, rib¹ has presumptive embryonic/larval peripheral nervous system phenotype

(Jack and Myette, 1997)

raw¹, rib¹ has larval tracheal system phenotype

(Jack and Myette, 1997)

raw¹, rib¹ has embryonic/larval salivary gland | embryonic stage phenotype

(Jack and Myette, 1997)

Additional Comments

Genetic Interactions

Statement

Reference

The raw¹ salivary gland phenotype is partially suppressed by zip^mhc-c1.3; the salivary glands are more similar to wild type and raw¹ zip^mhc-c1.3 salivary glands accumulate a secreted product in their lumens (as in wild-type embryos). The phenotype is also partially suppressed by zip². The raw¹ dorsal closure phenotype is partially suppressed by zip²; a small amount of dorsal closure of the epidermis is seen. The severity of the branching and forking of cuticular hairs is seen in raw¹ embryos is reduced by zip².

(Blake et al., 1999)

The ventral cuticle in raw¹ Jra^IA109 double mutant embryos develops normally, as it does in Jra^IA109 single mutants. The ventral cuticle in raw¹ mutant embryos expressing aop^ACT.Scer\UAS under the control of Scer\GAL4^69B develops normally, as it does in embryos expressing aop^ACT.Scer\UAS under the control of Scer\GAL4^69B in a raw⁺ background.

(Byars et al., 1999)

rib¹ raw¹ double mutant embryos have rudimentary salivary glands and have smaller tracheae than in rib¹ single mutant embryos. The PNS is grossly disorganised in rib¹ raw¹ double mutant embryos, with nearly the entire PNS of each segment being clustered along the dorsal edge of the epidermis. Axons are not apparent.

(Jack and Myette, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

raw^k01021

(Byars et al., 1999)

raw⁰²⁴¹⁸

(Byars et al., 1999)

raw^k03514

(Byars et al., 1999)

raw^k03703

(Liu et al., 1999)

Rescued by

raw¹ is rescued by raw^UAS.Tag:FLAG/Scer\GAL4^69B

(Yang and Su, 2011)

Not rescued by

raw¹ is not rescued by Scer\GAL4^how-24B/raw^UAS.Tag:FLAG

(Yang and Su, 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

2749

raw¹ cn¹ bw¹ speck¹/CyO

Kyoto

107458

raw¹ cn¹ bw¹ speck¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

raw¹

(Ríos-Barrera et al., 2015, Yang and Su, 2011, Christensen and Cook, 2006.8.30, Christensen and Cook, 2006.8.30, Christensen and Cook, 2006.8.30, Polaski et al., 2006)

raw^IG

(Byars et al., 1999, Jack and Myette, 1999, Jack and Myette, 1997, Arora et al., 1995, Tearle and Nusslein-Volhard, 1987)

Name Synonyms

Secondary FlyBase IDs

References (14)

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