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General Information
Symbol
Dmel\raw1
Species
D. melanogaster
Name
FlyBase ID
FBal0014432
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
rawIG
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C8711779T

Reported nucleotide change:

C?T

Amino acid change:

Q609term | raw-PA; Q425term | raw-PB; Q609term | raw-PC; Q452term | raw-PD; Q609term | raw-PE

Reported amino acid change:

?610term

Comment:

Position of mutation on reference sequence inferred by FlyBase curator based on author statement.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: ?609term.

Nucleotide substitution: C?T.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The salivary gland of mutant embryos is shorter and wider than wild type. Malpighian tubules are short and wide. The hindgut is distorted. The raw1 Malpighian tubule phenotype is partially suppressed by zipmhc-c1.3 or zip2. The amnioserosa remains completely exposed in mutant embryos, due to a failure in dorsal closure. Cuticular hairs are generally disorganised in appearance in mutant embryos, may be inappropriately clustered and are often forked or branched.

Homozygous embryos have a dorsal epidermal hole, defects in head involution and germband retraction and poorly differentiated ventral cuticle. The ventral cuticle does not have dorsal hairs, indicating that it is not transformed to a dorsal fate. Wild-type patterns of asymmetric gastrulation are seen in these embryos. Elongated neuronal cell bodies, thickened axon bundles and fasciculation defects are seen in the peripheral nervous system. The amnioserosal membrane is readily apparent in germband-retracted, stage 13-14 homozygous embryos and the cells in the amnioserosa are not apoptotic. Elongated leading edge cells are seen in homozygous embryos undergoing dorsal closure.

Almost all epidermal cells remain polygonal during dorsal closure in homozygous embryos, in contrast to wild-type where the cells elongate dorsoventrally. The epidermis appears wrinkled and the dorsal edge is folded inward. The shape of the salivary glands and hindgut is abnormal (although the hindgut phenotype is variable and the hindgut often resembles the wild-type), and the cells of both these organs are less columnar than in wild-type; salivary gland cells are approximately the same width as wild-type, but shorter than wild-type, and hindgut cells are both wider and shorter than wild-type. The salivary gland cells lack the wedge shape characteristic of wild-type salivary gland cells.

Malpighian tubules have a greater circumference than normal at their proximal end in homozygous embryos, giving them a gourd-like shape. The lumen of the tubules is greatly enlarged compared to wild-type. The epidermis never closes dorsally, the central nervous system fails to retract and midgut constrictions are incomplete.

embryonic lethal dorsal closure and cuticle differentiation defective.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

raw1/raw[+] is a suppressor of partially lethal - majority die phenotype of slprBS06

NOT Suppressor of
Statement
Reference
Phenotype Manifest In
NOT Enhanced by
Statement
Reference

raw1 has embryo | dorsal phenotype, non-enhanceable by tkv7

Suppressed by
Statement
Reference

raw1 has Malpighian tubule phenotype, suppressible by zipmhc-c1.3

raw1 has Malpighian tubule phenotype, suppressible by zip2

raw1 has denticle phenotype, suppressible by zip2

raw1 has dorsal hair phenotype, suppressible by zip2

raw1 has embryo | dorsal closure stage phenotype, suppressible by zip2

NOT suppressed by
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

The raw1 salivary gland phenotype is partially suppressed by zipmhc-c1.3; the salivary glands are more similar to wild type and raw1 zipmhc-c1.3 salivary glands accumulate a secreted product in their lumens (as in wild-type embryos). The phenotype is also partially suppressed by zip2. The raw1 dorsal closure phenotype is partially suppressed by zip2; a small amount of dorsal closure of the epidermis is seen. The severity of the branching and forking of cuticular hairs is seen in raw1 embryos is reduced by zip2.

The ventral cuticle in raw1 JraIA109 double mutant embryos develops normally, as it does in JraIA109 single mutants. The ventral cuticle in raw1 mutant embryos expressing aopACT.Scer\UAS under the control of Scer\GAL469B develops normally, as it does in embryos expressing aopACT.Scer\UAS under the control of Scer\GAL469B in a raw+ background.

rib1 raw1 double mutant embryos have rudimentary salivary glands and have smaller tracheae than in rib1 single mutant embryos. The PNS is grossly disorganised in rib1 raw1 double mutant embryos, with nearly the entire PNS of each segment being clustered along the dorsal edge of the epidermis. Axons are not apparent.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (14)