FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\ruPLLb
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General Information
Symbol
Dmel\ruPLLb
Species
D. melanogaster
Name
FlyBase ID
FBal0118064
Feature type
allele
Associated gene
Dmel\ru
Associated Insertion(s)
Carried in Construct
Also Known As
rho3pllb
Key Links
Allele class

amorphic allele - molecular evidence

amorphic allele - genetic evidence

Mutagen
    Nature of the Allele
    Allele class

    amorphic allele - genetic evidence

    (Yu et al., 2010)

    amorphic allele - molecular evidence

    (Yu et al., 2010)
    Mutagen
    Progenitor genotype
    Cytology
    Description
    Mutations Mapped to the Genome
    Curation Data
    Type
    Location
    Additional Notes
    References
    Variant Molecular Consequences
    Associated Sequence Data
    DDBJ / EMBL / GenBank
    DNA sequence
    Protein sequence
     
    UniProtKB/Swiss-Prot
      UniProtKB/TrEMBL
        Expression Data
        Reporter Expression
        Additional Information
        Statement
        Reference
         
        Marker for
        Reflects expression of
        Reporter construct used in assay
        Human Disease Associations
        Disease Ontology (DO) Annotations
        Models Based on Experimental Evidence ( 0 )
        Disease
        Evidence
        References
        Modifiers Based on Experimental Evidence ( 0 )
        Disease
        Interaction
        References
        Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
         
        Disease-implicated variant(s)
         
        Phenotypic Data
        Phenotypic Class
        Phenotype Manifest In
        Detailed Description
        Statement
        Reference

        In ruPLLb mutant third instar larval eye disc clones (generated using the eyFLP system) the general program of photoreceptor differentiation appears to be unperturbed and axons are correctly targeted to the lamina. However defects are seen in lamina cartridge neuron differentiation. Electroretinogram recordings from adult flies show that the eyes of ruPLLb mutants are able to properly depolarize in response to light. The 'on/off transients" (representing the activity of post-synaptic lamina cartridge neurons) that are normally seen in wild type are absent from the ECG.

        (Yogev et al., 2010)

        In stage 16 ruPLLb mutant embryos, a significant number of ganglionic branch trachea fail to turn posteriorly and dorsally at the ventral midline and instead cross the midline or remain lingering on it. Associated glia are unaffected.

        (Gallio et al., 2004)

        In ruPLLb flies, 84% of ommatidia have reduced numbers of photoreceptor cells and those with correct numbers show rotation defects.

        (Schlesinger et al., 2004)

        ruPLLb mutant flies have malformed eyes, with collapsed and blistered ommatidia.

        (Gallio et al., 2002)

        The regular array of facets in the eyes of homozygotes produces a rough eye phenotype. There is variable loss of photoreceptors in these mutant eyes, however few defects if any are seen in the recruitment of photoreceptors. Cone cells are dramatically under-recruited, and some pigment cells are seen to be missing.

        (Wasserman et al., 2000)
        External Data
        Interactions
        Show genetic interaction network for Enhancers & Suppressors
        Phenotypic Class
        Phenotype Manifest In
        Additional Comments
        Genetic Interactions
        Statement
        Reference

        Expression of rhoScer\UAS.T:Avic\GFP-EGFP in the R2, R5 and R8 photoreceptors under the control of Scer\GAL4MT14 fails to suppress the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae.

        Expression of rho::ruR3L1-R1.Scer\UAS.T:Avic\GFP-EGFP (rho targeted to the ER) in the R2, R5 and R8 photoreceptors under the control of Scer\GAL4MT14 suppresses the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae.

        Expression of rho::ruR1L1-R3.Scer\UAS.T:Avic\GFP-EGFP (ru that is not targeted to the ER) in the R2, R5 and R8 photoreceptors under the control of Scer\GAL4MT14 fails to suppress the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae.

        One copy of Syx5AR113 partially rescues the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae. Neuronal markers are detected in a small population of cells at the posterior of the lamina.

        Expression of rhoScer\UAS.T:Avic\GFP,T:Uuuu\KDEL in the R2, R5 and R8 photoreceptors under the control of Scer\GAL4MT14 fails to suppress the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae.

        Expression of spic.Scer\UAS.T:Arus\HRP,T:SS-wg in the R8 photoreceptors under the control of Scer\GAL4MT14 fails to suppress the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae.

        Expression of spic.Scer\UAS.T:Ivir\HA1 in the R8 photoreceptors under the control of Scer\GAL4MT14 fails to suppress the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae.

        (Yogev et al., 2010)

        Mutants homozygous or heterozygous for the ruPLLb allele have enlarged cardiac chambers.

        (Yu et al., 2010)

        The effects of sliGA20 and ruPLLb on migration of ganglionic branch trachea approaching the ventral midline during stage 16 appear to be additive.

        (Gallio et al., 2004)

        The eye phenotypes seen in ruPLLb homozygotes are partially rescued by sl9/sl9 : Most ommatidia have normal numbers of photoreceptor cells, but 50% show rotation defects.

        (Schlesinger et al., 2004)
        Xenogenetic Interactions
        Statement
        Reference

        Pstu\aarAScer\UAS.cGa; Scer\GAL4GMR.PF partially rescues the eye defects seen in ruPLLb mutant flies.

        (Gallio et al., 2002)
        Complementation and Rescue Data
        Rescued by

        ruPLLb is rescued by ruUAS.EGFP/Scer\GAL4GMR.PF

        (Yogev et al., 2010)

        ruPLLb is rescued by ruUAS.EGFP/Scer\GAL4MT14

        (Yogev et al., 2010)

        ruPLLb is rescued by ruhs.PW

        (Wasserman et al., 2000)
        Partially rescued by

        ruPLLb is partially rescued by ruUAS.EGFP/Scer\GAL4lz-gal4

        (Yogev et al., 2010)
        Not rescued by

        ruPLLb is not rescued by ruUAS.EGFP/Scer\GAL4hs.2sev

        (Yogev et al., 2010)

        ruPLLb is not rescued by ruUAS.EGFP/Scer\GAL4sca-537.4

        (Yogev et al., 2010)

        ruPLLb is not rescued by ruUAS.GFP.Tag:ER(KDEL)/Scer\GAL4MT14

        (Yogev et al., 2010)
        Comments

        Expression of ruScer\UAS.T:Avic\GFP-EGFP in all photoreceptors (R1 - R8) under the control of Scer\GAL4GMR.PF fully rescues the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae.

        Expression of ruScer\UAS.T:Avic\GFP-EGFP in the R2, R5 and R8 photoreceptors under the control of Scer\GAL4MT14 fully rescues the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae.

        Expression of ruScer\UAS.T:Avic\GFP-EGFP in the R1, R6 and R7 photoreceptors under the control of Scer\GAL4lz-gal4 partially rescues the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae. 20% of the wild type number of lamina cells express differentiation markers.

        Expression of ruScer\UAS.T:Avic\GFP-EGFP in the R3, R4 and R7 photoreceptors under the control of Scer\GAL4hs.2sev fails to rescue the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae.

        Expression of ruScer\UAS.T:Avic\GFP-EGFP in the R4 and weakly in the R3 photoreceptors under the control of Scer\GAL4hs.2sev fails to rescue the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae.

        Expression of ruScer\UAS.T:Avic\GFP-EGFP in the R8 photoreceptors under the control of Scer\GAL4sca-537.4 fails to rescue the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae.

        Expression of ruScer\UAS.T:Avic\GFP.T:Uuuu\KDEL in the R8 photoreceptors under the control of Scer\GAL4MT14 fails to rescue the lamina cartridge neuron differentiation defects seen in the eye discs of ruPLLb mutant third instar larvae.

        (Yogev et al., 2010)
        Images (0)
        Mutant
        Wild-type
        Stocks (0)
        Notes on Origin
        Discoverer
        External Crossreferences and Linkouts ( 0 )
        Synonyms and Secondary IDs (8)
        Reported As
        Symbol Synonym
        rho-3
        (Yogev et al., 2010)
        rho-3PLLb
        (Yu et al., 2010)
        rho-3roughoid
        (Gallio et al., 2002)
        rho3PLLb
        (Fernandes et al., 2017)
        rho3pLLb
        (Urban et al., 2004)
        rho3pllb
        (Gallio et al., 2004)
        ru/rho3pLLb
        (Gallio et al., 2002)
        ruPLLb
        (Shwartz et al., 2013, Yogev et al., 2010, Birkholz et al., 2009, Schlesinger et al., 2004)
        Name Synonyms
        Secondary FlyBase IDs
          References (10)