FlyBase Allele Report: Dmel\alph[S-355]

General Information

Symbol

Dmel\alph^S-355

Species

D. melanogaster

Name

FlyBase ID

FBal0120640

Feature type

allele

Associated gene

Dmel\alph

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: G173S.

(Baril and Therrien, 2006)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:29,593,867..29,593,867 [+]

Nucleotide change:

G29593867A

Amino acid change:

Reported amino acid change:

G173S

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Baril and Therrien, 2006)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Therrien et al., 2000)

partially lethal - majority live (with alph^S-331)

(Therrien et al., 2000)

partially lethal - majority live (with alph^XS-88)

(Therrien et al., 2000)

viable

(Baril and Therrien, 2006)

visible (with alph^S-331)

(Therrien et al., 2000)

visible (with alph^XS-88)

(Therrien et al., 2000)

Phenotype Manifest In

eye (with alph^S-331)

(Therrien et al., 2000)

eye (with alph^XS-88)

(Therrien et al., 2000)

wing (with alph^S-331)

(Therrien et al., 2000)

wing (with alph^XS-88)

(Therrien et al., 2000)

wing vein (with alph^S-331)

(Therrien et al., 2000)

wing vein (with alph^XS-88)

(Therrien et al., 2000)

Detailed Description

Statement

Reference

Heterozygotes with pp2c99B^XS-88 or pp2c99B^S-331 are sub-viable with rough eyes and defects including missing wing veins and atrophied wings.

(Therrien et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

alph^S-355/pp2c99B[+] is an enhancer of visible phenotype of Ras85D^V12.sev

(Therrien et al., 2000)

Suppressor of

Statement

Reference

alph^S-355/alph[+] is a suppressor of lethal phenotype of slpr^BS06

(Baril et al., 2009)

alph^S-355/alph[+] is a suppressor | partially of lethal - all die during P-stage phenotype of hep^G0107

(Baril et al., 2009)

alph^S-355/alph[+] is a suppressor | partially of lethal phenotype of lic^G0252

(Baril et al., 2009)

alph^S-355/pp2c99B[+] is a suppressor of visible phenotype of ksr::tor^{ΔNksr.hs.sev}

(Therrien et al., 2000)

alph^S-355/pp2c99B[+] is a suppressor of lethal phenotype of Raf¹²

(Therrien et al., 2000)

Phenotype Manifest In

Enhancer of

Statement

Reference

alph^S-355 is an enhancer of photoreceptor cell R7 | increased number phenotype of rl^Sem.sev

(Baril and Therrien, 2006)

alph^S-355 is an enhancer of eye phenotype of Ras85D^V12.sev

(Baril and Therrien, 2006)

alph^S-355/pp2c99B[+] is an enhancer of eye phenotype of Ras85D^V12.sev

(Therrien et al., 2000)

NOT Enhancer of

Statement

Reference

alph^S-355 is a non-enhancer of eye phenotype of phyl^hs.sev

(Baril and Therrien, 2006)

Suppressor of

Statement

Reference

alph^S-355 is a suppressor of photoreceptor neuron phenotype of ksr::tor^{ΔNksr.hs.sev}

(Baril and Therrien, 2006)

alph^S-355/pp2c99B[+] is a suppressor of eye phenotype of ksr::tor^{ΔNksr.hs.sev}

(Therrien et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

Introduction of a heterozygous alph^S-355 allele into the lic^G0252 background suppresses hemizygous male lethality.

Introduction of a heterozygous alph^S-355 allele into the hep^G0107 background suppresses hemizygous male lethality.

alph^S-355 restores viability to slpr^BS06/Y males.

(Baril et al., 2009)

rl^Sem.sev heterozygous mutants in combination with alph^S-355 exhibit a rough eye phenotype. They exhibit an average of 3.39 R7 cells per ommatidium, compared to 1 R7 cell per ommatidium in wild-type.

ksr::tor^{ΔNksr.hs.sev}/alph^S-355 mutants exhibit a 20% loss of ommatidial R7 cells, indicating that alph^S-355 has suppressed the ksr::tor^{ΔNksr.hs.sev} phenotype. This effect is not specific to the R7 cells as the loss of outer photoreceptors is suppressed in this double mutant.

The rough eye phenotype of Ras85D^V12.sev mutants is increased when transheterozygous with alph^S-355.

phyl^hs.sev heterozygous mutants in combination with alph^S-355 exhibit a rough eye phenotype. They exhibit an average of 1.70 R7 cells per ommatidium, compared to 1 R7 cell per ommatidium in wild-type. This however is no higher than in phyl^hs.sev heterozygous mutants.

(Baril and Therrien, 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

alph^S-331

(Therrien et al., 2000)

alph^XS-88

(Therrien et al., 2000)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

59008

alph^S-355/TM3, P{sE-KDN.T}3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

S-355

(Baril and Therrien, 2006)

SK3-1^S-355

(Therrien et al., 2000)

alph^S-355

(Baril et al., 2009)

pp2c99B^S-355

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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