FlyBase Allele Report: Dmel\neur[UAS.cLa]

General Information

Symbol

Dmel\neur^UAS.cLa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Lai

FlyBase ID

FBal0121662

Feature type

allele

Associated gene

Dmel\neur

Associated Insertion(s)

Carried in Construct

P{UAS-neur.L}

Also Known As

UAS-neur

Key Links

Transgenic product class

cDNA

(Lai and Rubin, 2001)

wild_type

(Lai and Rubin, 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Lai and Rubin, 2001)

wild_type

(Lai and Rubin, 2001)

Mutagen

in vitro construct

(Lai and Rubin, 2001)

Progenitor genotype

Carried in construct

P{UAS-neur.L}

Cytology

Description

UASt sequences drive expression of a MluI/MfeI fragment of the neur cDNA.

(Lai and Rubin, 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

neur

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^GMR.PF

(Lai et al., 2001)

visible, with Scer\GAL4^sca-537.4

(Lai and Rubin, 2001)

Phenotype Manifest In

campaniform sensillum, with Scer\GAL4^dpp.blk1

(Lai et al., 2005)

campaniform sensillum of dorsal radius, with Scer\GAL4^Bx-MS1096

(Lai and Rubin, 2001)

campaniform sensillum of dorsal radius, with Scer\GAL4^dpp.blk1

(Lai et al., 2001)

campaniform sensillum of dorsal radius | increased number, with Scer\GAL4^Bx-MS1096

(Lai and Rubin, 2001)

cone cell, with Scer\GAL4^GMR.PF

(Lai and Rubin, 2001)

eye, with Scer\GAL4^ey.PS

(Lai and Rubin, 2001)

eye, with Scer\GAL4^GMR.PF

(Lai and Rubin, 2001)

eye disc, with Scer\GAL4^GMR.PF

(Lai and Rubin, 2001)

interommatidial bristle | increased number, with Scer\GAL4^GMR.PF

(Lai and Rubin, 2001, Lai et al., 2001)

macrochaeta & head, with Scer\GAL4^sca-537.4

(Lai and Rubin, 2001)

mesonotum, with Scer\GAL4^sca-537.4

(Lai and Rubin, 2001)

ommatidium, with Scer\GAL4^GMR.PF

(Lai and Rubin, 2001)

photoreceptor neuron, with Scer\GAL4^GMR.PF

(Lai and Rubin, 2001)

sensory mother cell, with Scer\GAL4^sca-537.4

(Lai and Rubin, 2001)

tormogen cell, with Scer\GAL4^neur-P72

(Le Borgne and Schweisguth, 2003)

trichogen cell, with Scer\GAL4^neur-P72

(Le Borgne and Schweisguth, 2003)

wing vein, with Scer\GAL4^Bx-MS1096

(Lai and Rubin, 2001)

wing vein, with Scer\GAL4^dpp.blk1

(Lai et al., 2005)

wing vein L5, with Scer\GAL4^VMQ

(Lai and Rubin, 2001)

Detailed Description

Statement

Reference

Expression of neur^Scer\UAS.cLa, under the control of Scer\GAL4^dpp.blk1, causes a mild loss of wing veins and campaniform sensilla.

(Lai et al., 2005)

Overexpression of neur^Scer\UAS.cLa, driven by Scer\GAL4^neur-P72, in pI cells results in a weak double-socket phenotype associated with a shaft-to-socket transformation.

(Le Borgne and Schweisguth, 2003)

When a single copy of neur^Scer\UAS.cLa is driven by Scer\GAL4^sca-537.4 mild notum and haed sensory organ loss is seen. A loss of Sensory Organ Precursors (SOPs) is also seen in wing discs. When a single copy of neur^Scer\UAS.cLa is driven by Scer\GAL4^Bx-MS1096 in the wing, wing vein truncation is seen. The wing vein L3 campaniform sensillae are also missing. At higher levels of misexpression, a recovery of vein length concomitant with a strong thickening of veins is seen. Clusters of L3 campaniform sensillae are also seen. When neur^Scer\UAS.cLa is driven by Scer\GAL4^VMQ, wings display a mild truncation of wing vein 5 and a small amount of wing vein thickening.

(Lai and Rubin, 2001)

When a single copy of neur^Scer\UAS.cLa is driven by Scer\GAL4^GMR.PF, adults exhibit a rough eye phenotype. Mild ommatidial disarray and tufting of interommatidial bristles are seen. When three copies are present, eyes show extreme ommatidial bristle tufting and some fusion and general ommatidial disarray. In the eye discs of these developing flies, photoreceptor and cone cell patterning in the posterior of the disc is abnormal; mild neural hypertrophy and fusion of clusters are apparent. When neur^Scer\UAS.cLa is driven by Scer\GAL4^ey.PS in the eye only minor defects are seen in the overall appearance of the eye.

(Lai and Rubin, 2001)

neur^Scer\UAS.cLa; Scer\GAL4^GMR.PF flies have extra interommatidial bristles. The wings of neur^Scer\UAS.cLa/neur^Scer\UAS.cLa; Scer\GAL4^dpp.blk1 flies lack most of the L3 campaniform sensilla, but little effect is seen on terminal vein differentiation.

(Lai et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, neur^UAS.cLa has visible phenotype, suppressible by eff^s1782/eff[+]

(Lai et al., 2001)

Enhancer of

Statement

Reference

neur^UAS.cLa/Scer\GAL4^VMQ is an enhancer of visible phenotype of Delta^9P

(Lai and Rubin, 2001)

neur^UAS.cLa/Scer\GAL4^VMQ is an enhancer of visible phenotype of N^Ax-M1

(Lai and Rubin, 2001)

neur^UAS.cLa/Scer\GAL4^VMQ is an enhancer of visible phenotype of H^E31

(Lai and Rubin, 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, neur^UAS.cLa has interommatidial bristle | increased number phenotype, suppressible by eff^s1782/eff[+]

(Lai et al., 2001)

Enhancer of

Statement

Reference

neur^UAS.cLa/Scer\GAL4^VMQ is an enhancer of wing vein phenotype of Delta^9P

(Lai and Rubin, 2001)

neur^UAS.cLa/Scer\GAL4^VMQ is an enhancer of wing vein L5 phenotype of N^Ax-M1

(Lai and Rubin, 2001)

neur^UAS.cLa/Scer\GAL4^VMQ is an enhancer of wing vein phenotype of N^Ax-M1

(Lai and Rubin, 2001)

neur^UAS.cLa/Scer\GAL4^VMQ is an enhancer of wing vein L5 phenotype of H^E31

(Lai and Rubin, 2001)

neur^UAS.cLa/Scer\GAL4^VMQ is an enhancer of wing vein phenotype of H^E31

(Lai and Rubin, 2001)

Suppressor of

Statement

Reference

neur^UAS.cLa/Scer\GAL4^dpp.blk1 is a suppressor | partially of campaniform sensillum phenotype of Scer\GAL4^dpp.blk1, mib1^{ΔRF.UAS.Tag:polyHis,Tag:MYC}

(Lai et al., 2005)

neur^UAS.cLa/Scer\GAL4^dpp.blk1 is a suppressor | partially of wing margin phenotype of Scer\GAL4^dpp.blk1, mib1^{ΔRF.UAS.Tag:polyHis,Tag:MYC}

(Lai et al., 2005)

neur^UAS.cLa/Scer\GAL4^dpp.blk1 is a suppressor | partially of wing vein L3 phenotype of Scer\GAL4^dpp.blk1, mib1^{ΔRF.UAS.Tag:polyHis,Tag:MYC}

(Lai et al., 2005)

Other

Statement

Reference

Delta^9P, Scer\GAL4^VMQ, neur^UAS.cLa has wing phenotype

(Lai and Rubin, 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of neur^Scer\UAS.cLa partially rescues the wing phenotype of flies that express mib1^{ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} under the control of Scer\GAL4^dpp.blk1. This phenotype is almost completely suppressed when both mib1^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} and two copies of neur^Scer\UAS.cLa are expressed under the control of Scer\GAL4^dpp.blk1 alongside mib1^{ΔRF.Scer\UAS.T:Zzzz\His6,T:Hsap\MYC}.

(Lai et al., 2005)

The combination of Dl^9P and neur^Scer\UAS.cLa ( when driven by Scer\GAL4^VMQ) produces a blistered wing phenotype. heterozygous Df(1)N-81k1 flies have small notches at the distal portion of the wing. This is made more variable by the addition of neur^Scer\UAS.cLa, when driven by Scer\GAL4^VMQ.

(Lai and Rubin, 2001)

The extra interommatidial bristle phenotype of neur^Scer\UAS.cLa; Scer\GAL4^GMR.PF flies is dominantly suppressed by eff^s1782.

(Lai et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

neur^UAS.cLa/Scer\GAL4^sca-537.4 rescues neur^A101

(Lai et al., 2005)

Comments

The presence of neur^Scer\UAS.cLa completely rescues the vein thickening and notching phenotypes due to neur^{αRING.Scer\UAS}, Scer\GAL4^dpp.blk1, and partially suppresses the formation of ectopic campaniform sensilla. Paritally rescues: neur^{αRING.Scer\UAS}

(Lai et al., 2001)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26271

w^*; P{UAS-neur.L}2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

neur^Scer\UAS.cLa

neur^UAS.cLa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Lai

(Lai and Rubin, 2001)

Secondary FlyBase IDs

References (15)

Report Sections

Open Close