FlyBase Allele Report: Dmel\Dronc[C318G.UAS.EGFP]

General Information

Symbol

Dmel\Dronc^{C318G.UAS.EGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0122050

Feature type

allele

Associated gene

Dmel\Dronc

Associated Insertion(s)

Carried in Construct

P{UAS-Dronc.EGFP.C318G}

Also Known As

UAS-Dronc^DN

Key Links

Transgenic product class

missense_variant

(Quinn et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Quinn et al., 2000)

Mutagen

in vitro construct

(Quinn et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-Dronc.EGFP.C318G}

Cytology

Description

UASt regulatory sequences drive expression of an inactive form of Dronc tagged with EGFP.

(Quinn et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Dronc

Tagged with

EGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4⁴⁷⁷

(Williams et al., 2006)

visible, with Scer\GAL4^sca-P309

(Kanuka et al., 2005)

visible | adult stage, with Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Phenotype Manifest In

dendrite, with Scer\GAL4⁴⁷⁷

(Williams et al., 2006)

eo neuron | increased number, with Scer\GAL4^sca-P309

(Kanuka et al., 2005)

larval dorsal multidendritic neuron ddaC, with Scer\GAL4⁴⁷⁷

(Williams et al., 2006)

macrochaeta | increased number, with Scer\GAL4^sca-P309

(Kanuka et al., 2005)

sensory mother cell | increased number, with Scer\GAL4^sca-P309

(Kanuka et al., 2005)

thecogen cell | increased number, with Scer\GAL4^sca-P309

(Kanuka et al., 2005)

wing, with Scer\GAL4^ap-md544

(Shinoda et al., 2019)

Detailed Description

Statement

Reference

The expression of Dronc^{C318G.UAS.EGFP} under the control of Scer\GAL4^C10 does not affect the size of the adult wing, femur, tibia or tarsal segment 1.

Expression under the control of Scer\GAL4^ap-md544 leads to an opaque wing phenotype, indicative of apoptosis, but does not induce curly wings, which would indicate growth inhibition.

(Shinoda et al., 2019)

Overexpression of Nc^{ΔN.C-A.Scer\UAS} in the ddaC neurons under the control of Scer\GAL4⁴⁷⁷ results in a marked suppression of dendritic pruning. At 14 hours after pupal formation, the dendrites are detached from the soma but still largely cover the same 'footprint' as in third instar larvae. The morphology of detached branches varies: some show proximal swellings, whereas others are thinned and beaded along their entire length.

(Williams et al., 2006)

Ectopic macrochaetae form on the scutellum in 55.2% of cases when Dronc^{C318G.UAS.EGFP} is expressed under the control of Scer\GAL4^sca-P309. In contrast, the ectopic expression of Dronc^{C318G.UAS.EGFP} in SOP cells, driven by Scer\GAL4^{neur-GAL4-A101}, does not result in extra macrochaetae. Larvae expressing Dronc^{C318G.UAS.EGFP} under the control of Scer\GAL4^sca-P309 have one extra SOP cell in the scutellum region of the third instar wing disc. Analysis of the scutellum region of the pupal notum (24---30 h after pupal formation (APF)) shows that there is an extra neuron and thecogen present, which are derived from the extra SOP cell. No significant increase in macrochaetae number is seen when Dronc^{C318G.UAS.EGFP} is expressed under the control of Scer\GAL4^hs.PB following a heat shock at late third-instar (6-12 h APF), which is during SOP cell formation, or after it (24-30 h APF). At the stage prior to the appearance SOP cells, however, Dronc^{C318G.UAS.EGFP} expression leads to extra macrochaetae formation.

(Kanuka et al., 2005)

Expression of Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PF has little effect on the normal pattern of dying cells in the eye imaginal disc. No death during the pupal stage is seen in animals expressing Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP} under the control of Scer\GAL4^GMR.PF and adult flies have normal eyes.

(Quinn et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^nub.PU/Dronc^{C318G.UAS.EGFP} is a suppressor | partially of visible | adult stage phenotype of RpS3^Plac92, yki^B5

(Wada et al., 2021)

Scer\GAL4^nub.PU/Dronc^{C318G.UAS.EGFP} is a suppressor | partially of decreased size | adult stage phenotype of RpS3^Plac92, yki^B5

(Wada et al., 2021)

Phenotype Manifest In

Enhanced by

Statement

Reference

Dronc^{C318G.UAS.EGFP}, Scer\GAL4^sca-P309 has macrochaeta | increased number phenotype, enhanceable by sgg³²

(Kanuka et al., 2005)

Suppressed by

Statement

Reference

Dronc^{C318G.UAS.EGFP}, Scer\GAL4^sca-P309 has macrochaeta | increased number phenotype, suppressible by wg^Sp-1

(Kanuka et al., 2005)

Enhancer of

Statement

Reference

Dronc^{C318G.UAS.EGFP}, Scer\GAL4^sca-P309 is an enhancer of macrochaeta phenotype of IKKε^NIG.2615R, Scer\GAL4^sca-P309

(Koto et al., 2009)

Suppressor of

Statement

Reference

Scer\GAL4^nub.PU/Dronc^{C318G.UAS.EGFP} is a suppressor | partially of wing phenotype of RpS3^Plac92, yki^B5

(Wada et al., 2021)

Dronc^{C318G.UAS.EGFP}, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of IKKε^UAS.cKa, Scer\GAL4^GMR.PF

(Kuranaga et al., 2006)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP} in Scer\GAL4^sca-P309; ik2^{dsRNA.Scer\UAS} flies significantly enhances the short-bristle phenotype.

(Koto et al., 2009)

Expression of Dronc^DN.UAS.cUa partially suppresses the eye ablation phenotype seen in Scer\GAL4^GMR.PF>IKKε^UAS.cKa flies.

(Kuranaga et al., 2006)

The wg^Sp-1 mutation is able to suppress the increase in extra macrochaetae seen when Dronc^{C318G.UAS.EGFP} is expressed under the control of Scer\GAL4^sca-P309, while the sgg³² null mutation enhances this phenotype.

(Kanuka et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Dronc^{C318G.Scer\UAS.T:Avic\GFP-EGFP}

Dronc^{C318G.UAS.EGFP}

Nc^{C318G.Scer\UAS.T:Avic\GFP-EGFP}

Nc^{C318G.Scer\UAS.T:Avic\GFP-F64L.S65T}

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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