FlyBase Allele Report: Dmel\EcR[B1.UAS]

General Information

Symbol

Dmel\EcR^B1.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0122213

Feature type

allele

Associated gene

Dmel\EcR

Associated Insertion(s)

Carried in Construct

P{UAS-EcR.B1}

Also Known As

UAS-EcR-B1, UAS-EcRB1, UAS-EcR.B1, EcR-B1

Key Links

Transgenic product class

cDNA

(Lee et al., 2000)

wild_type

(Lee et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Lee et al., 2000)

wild_type

(Lee et al., 2000)

Mutagen

in vitro construct

(Lee et al., 2000)

Progenitor genotype

Carried in construct

P{UAS-EcR.B1}

Cytology

Description

UASt sequences drive expression of a 3.11 kb FspI/HindIII cDNA fragment containing the EcR-B1 isoform.

(Lee et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

EcR

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates brain cancer

modeled by brat^KK113206

(Jiang et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal sleep, with Scer\GAL4^{Mef2.247.Switch}

(Ishimoto and Kitamoto, 2010)

lethal, with Scer\GAL4^Act5C.PI

(Cherbas et al., 2003)

partially lethal, with Scer\GAL4^en-e16E

(Schubiger et al., 2003)

Phenotype Manifest In

border follicle cell, with Scer\GAL4^slbo.2.6

(Medioni and Noselli, 2005)

female germline cell | increased number, with Scer\GAL4^bab1-Pgal4-2

(König et al., 2011)

germarium cap cell | increased number, with Scer\GAL4^bab1-Pgal4-2

(König et al., 2011)

polar follicle cell, with Scer\GAL4^slbo.2.6

(Medioni and Noselli, 2005)

wing vein, with Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

Detailed Description

Statement

Reference

Expression of EcR^B1.Scer\UAS in the developing wing under the control of Scer\GAL4^Bx-MS1096 results in mild ectopic wing vein formation.

(Kleefstra et al., 2012)

The number of niche cap cells and the number of germline cells containing a single spectrosome (SSCs) per germarium is increased in females expressing EcR^B1.Scer\UAS under the control of Scer\GAL4^bab1-Pgal4-2.

(König et al., 2011)

Expression of EcR^A.Scer\UAS under the control of Scer\GAL4^{Mef2.247.Switch} results in an increase in the total amount of sleep during the day and at night compared to controls. Average sleep bout duration is also increased but no change is seen in waking activity.

(Ishimoto and Kitamoto, 2010)

Expression of EcR^B1.Scer\UAS in border cells, driven by Scer\GAL4^slbo.2.6, causes a border cell cluster migration defect in over 50-60% of egg chambers. However, the apical cap of these egg chambers forms and is shed normally.

(Medioni and Noselli, 2005)

Expression of EcR^B1.Scer\UAS under the control of Scer\GAL4^en-e16E results in some decrease in the hatching rate, but no lethality during metamorphosis; 85% of eggs laid eclose as adults.

(Schubiger et al., 2003)

Expression of EcR^B1.Scer\UAS when driven by Scer\GAL4^Tab2-201Y does not result in any detectable abnormalities.

(Lee et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

EcR^B1.UAS, Scer\GAL4^Tab2-201Y is an enhancer of abnormal neuroanatomy phenotype of Hr39^C13, Scer\GAL4^Tab2-201Y

(Boulanger et al., 2011)

EcR^B1.UAS, Scer\GAL4^Tab2-201Y is an enhancer of abnormal neuroanatomy phenotype of Hr39^UAS.cBa, Scer\GAL4^Tab2-201Y

(Boulanger et al., 2011)

Suppressor of

Statement

Reference

EcR^B1.UAS, Scer\GAL4^Tab2-201Y is a suppressor of abnormal neuroanatomy | adult stage phenotype of Scer\GAL4^Tab2-201Y, orion^GD6447

(Boulanger et al., 2021)

Scer\GAL4^Tab2-201Y/EcR^B1.UAS is a suppressor of abnormal neuroanatomy | somatic clone | pupal stage phenotype of plum^Δ1

(Yu et al., 2013)

Scer\GAL4^elav.PU/EcR^B1.UAS is a suppressor | partially of abnormal neuroanatomy | larval stage phenotype of plum^Δ1/plum^Δ2

(Yu et al., 2013)

Scer\GAL4^Tab2-201Y/EcR^B1.UAS is a suppressor | partially of abnormal neuroanatomy phenotype of babo^unspecified

(Boulanger et al., 2011)

Scer\GAL4^Tab2-201Y/EcR^B1.UAS is a suppressor | partially of abnormal neuroanatomy phenotype of Hr39^c739, babo^unspecified

(Boulanger et al., 2011)

Scer\GAL4^Tab2-201Y/EcR^B1.UAS is a suppressor of abnormal neuroanatomy | somatic clone phenotype of ftz-f1^ex7

(Boulanger et al., 2011)

EcR^B1.UAS, Scer\GAL4^en-e16E is a suppressor of visible phenotype of Scer\GAL4^en-e16E, pzg^RNAi.UAS

(Kugler et al., 2011)

Scer\GAL4^Tab2-201Y/EcR^B1.UAS is a suppressor of abnormal neuroanatomy | somatic clone phenotype of SMC1^LL01162

(Schuldiner et al., 2008)

Phenotype Manifest In

Enhancer of

Statement

Reference

EcR^B1.UAS, Scer\GAL4^Bx-MS1096 is an enhancer of wing vein phenotype of G9a^UAS.cKa, Scer\GAL4^Bx-MS1096

(Kleefstra et al., 2012)

EcR^B1.UAS, Scer\GAL4^Tab2-201Y is an enhancer of gamma Kenyon cell phenotype of Hr39^C13, Scer\GAL4^Tab2-201Y

(Boulanger et al., 2011)

EcR^B1.UAS, Scer\GAL4^Tab2-201Y is an enhancer of gamma Kenyon cell phenotype of Hr39^UAS.cBa, Scer\GAL4^Tab2-201Y

(Boulanger et al., 2011)

Suppressor of

Statement

Reference

EcR^B1.UAS, Scer\GAL4^Tab2-201Y is a suppressor | partially of adult gamma Kenyon cell phenotype of Scer\GAL4^Tab2-201Y, orion^GD6447

(Boulanger et al., 2021)

EcR^B1.UAS, Scer\GAL4^Tab2-201Y is a suppressor | partially of axon | adult stage phenotype of Scer\GAL4^Tab2-201Y, orion^GD6447

(Boulanger et al., 2021)

EcR^B1.UAS, Scer\GAL4^Tab2-201Y is a suppressor of gamma Kenyon cell | somatic clone phenotype of Scer\GAL4^Tab2-201Y, plum^Δ1

(Yu et al., 2013)

Scer\GAL4^elav.PU/EcR^B1.UAS is a suppressor | partially of embryonic/larval neuromuscular junction | larval stage phenotype of plum^Δ1/plum^Δ2

(Yu et al., 2013)

Scer\GAL4^Tab2-201Y/EcR^B1.UAS is a suppressor | partially of gamma Kenyon cell phenotype of babo^unspecified

(Boulanger et al., 2011)

Scer\GAL4^Tab2-201Y/EcR^B1.UAS is a suppressor | partially of gamma Kenyon cell phenotype of Hr39^c739, babo^unspecified

(Boulanger et al., 2011)

Scer\GAL4^Tab2-201Y/EcR^B1.UAS is a suppressor of gamma Kenyon cell | somatic clone phenotype of ftz-f1^ex7

(Boulanger et al., 2011)

EcR^B1.UAS, Scer\GAL4^en-e16E is a suppressor of wing phenotype of Scer\GAL4^en-e16E, pzg^RNAi.UAS

(Kugler et al., 2011)

Scer\GAL4^Tab2-201Y/EcR^B1.UAS is a suppressor of mushroom body | somatic clone phenotype of SMC1^LL01162

(Schuldiner et al., 2008)

Scer\GAL4^Tab2-201Y/EcR^B1.UAS is a suppressor of adult mushroom body gamma-lobe | somatic clone phenotype of babo⁹

(Zheng et al., 2003)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Tab2-201Y/EcR^B1.UAS is a non-suppressor of adult gamma Kenyon cell | cell non-autonomous phenotype of orion¹

(Boulanger et al., 2021)

Scer\GAL4^Tab2-201Y/EcR^B1.UAS is a non-suppressor of axon | cell non-autonomous | adult stage phenotype of orion¹

(Boulanger et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of EcR^B1.Scer\UAS under the control of Scer\GAL4^Tab2-201Y largely rescues the γ neuron pruning defects seen in plum^Δ1 mushroom body neuroblast clones.

Expression of EcR^B1.Scer\UAS under the control of Scer\GAL4^elav.PU partially rescues the formation of ectopic connections at the neuromuscular junction in plum^Δ1/plum^Δ2 larvae.

(Yu et al., 2013)

Co-expression of EcR^B1.Scer\UAS enhances the G9a^Scer\UAS.cKa wing vein phenotype.

(Kleefstra et al., 2012)

Expression of EcR^B1.Scer\UAS (under the control of Scer\GAL4^Tab2-201Y) in ftz-f1^ex7 neuroblast clones restores normal γ neuron remodelling.

Expression of EcR^B1.Scer\UAS in Hr39^C13 neuroblast clones (under the control of Scer\GAL4^Tab2-201Y) restores wild-type γ neuron remodelling.

Expression of EcR^B1.Scer\UAS in Hr39^Scer\UAS.cBa neuroblast clones (under the control of Scer\GAL4^Tab2-201Y) restores wild-type γ neuron remodelling.

Restoration of EcR, through expression of EcR^B1.Scer\UAS in a babo^unspecified Hr39^c739 double mutant background, under the control of Scer\GAL4^Tab2-201Y partially rescues the γ-neuron remodelling defects in these mutants.

(Boulanger et al., 2011)

Co-expression of EcR^B1.Scer\UAS significantly suppresses the small wing phenotype caused by expression of pzg^{dsRNA.Scer\UAS} under the control of Scer\GAL4^en-e16E.

(Kugler et al., 2011)

The axon pruning defects seen in homozygous SMC1^LL01162 mushroom body clones are markedly suppressed by expression of EcR^B1.Scer\UAS under the control of Scer\GAL4^Tab2-201Y.

(Schuldiner et al., 2008)

The addition of EcR^B1.Scer\UAS driven by Scer\GAL4^Tab2-201Y partially rescues the gamma-lobe phenotype.

(Zheng et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

EcR^B1.UAS/Scer\GAL4^CCAP.PP rescues EcR^{B1-ΔC655.W650A.UAS}

(Lee et al., 2019)

Scer\GAL4^109(2)80/EcR^B1.UAS rescues EcR^{B1-ΔC655.W650A.UAS}

(Kirilly et al., 2009)

EcR^B1.UAS/Scer\GAL4^Crz.PC rescues EcR⁹⁹/EcR³¹

(Choi et al., 2006)

EcR^B1.UAS/Scer\GAL4^ppk.PG rescues EcR^{B1-ΔC655.F645A.UAS}

(Kuo et al., 2005)

EcR^B1.UAS/Scer\GAL4^Eh.0.8 rescues EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

EcR^B1.UAS/Scer\GAL4^Lsp2.PH rescues EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

EcR^B1.UAS/Scer\GAL4^GMR.PF rescues EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

Scer\GAL4^Tab2-201Y/EcR^B1.UAS rescues EcR³¹/EcR^W53st

(Lee et al., 2000)

Partially rescues

EcR^B1.UAS/Scer\GAL4^c42 partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Gautam et al., 2015)

EcR^B1.UAS/Scer\GAL4^ETH.Switch partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Gauthier et al., 2012)

EcR^B1.UAS/Scer\GAL4^Sgs3.PD partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

EcR^B1.UAS/Scer\GAL4^dpp.blk1 partially rescues EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

EcR^B1.UAS/Scer\GAL4^FMRFa.PS partially rescues EcR⁹⁹/EcR³¹

(Schubiger et al., 2003)

Fails to rescue

EcR^B1.UAS/Scer\GAL4^MsrA.602 fails to rescue EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

EcR^B1.UAS/Scer\GAL4^slbo.2.6 fails to rescue EcR^{B1-ΔC655.F645A.UAS}

(Cherbas et al., 2003)

Comments

Expressing EcR^B1.UAS under the control of Scer\GAL4^CCAP.PP fully rescues CCAP neuronal death in larvae and the pupal phenotype (failure in head eversion and shortened wings and legs) induced by expression of EcR^{B1-ΔC655.W650A.UAS} alone.

(Lee et al., 2019)

Co-expression of EcR^B1.Scer\UAS results in partial rescue of larval lethality, and significant rescue of the Malpighian tubule phenotypes of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^c42.

(Gautam et al., 2015)

Expression of EcR^B1.Scer\UAS under the control of Scer\GAL4^ETH.Switch in the presence of RU486 partially rescues the lethality and failure of ecdysis phenotypes seen in larvae expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^ETH.Switch in the presence of RU486.

(Gauthier et al., 2012)

Expression of EcR^B1.Scer\UAS, under the control of Scer\GAL4^Crz restores complete cell death in 73% of EcR³¹/EcR⁹⁹ tissue, while four to eleven neurons are detectable in the remaining specimens.

(Choi et al., 2006)

Co-expression of EcR^B1.Scer\UAS with EcR^{B1-ΔC655.F645A.Scer\UAS} in C4da neurons, under the regulation of Scer\GAL4^ppk.1.9 results in the complete rescue of EcR^{B1-ΔC655.F645A.Scer\UAS}-related dendritic pruning defects in C4da neurons.

Co-expression of EcR^B1.Scer\UAS with EcR^{B1-ΔC655.F645A.Scer\UAS} in C4da neurons, under the regulation of Scer\GAL4^ppk.PG results in the complete rescue of EcR^{B1-ΔC655.F645A.Scer\UAS}-related dendritic pruning defects in C4da neurons.

(Kuo et al., 2005)

Co-expression of EcR^B1.Scer\UAS rescues the lethality and eye defects caused by expression of EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^GMR.PF. Co-expression of EcR^B1.Scer\UAS rescues to normal viability and normal morphology animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Lsp2.PH or Scer\GAL4^Eh.0.8. Co-expression of EcR^B1.Scer\UAS rescues to nearly complete viability animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^dpp.blk1. Co-expression of EcR^B1.Scer\UAS partially rescues the glue secretion and puffing defects of animals expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^Sgs3.PD. Co-expression of EcR^B1.Scer\UAS does not rescue the border cell migration defects, chorion fragility or fertility of females expressing EcR^{B1-ΔC655.F645A.Scer\UAS} under the control of Scer\GAL4^slbo.2.6.

(Cherbas et al., 2003)

The failure of pruning of the larval Tv-neurons seen in EcR³¹/EcR⁹⁹ animals is often rescued by expression of EcR^B1.Scer\UAS under the control of Scer\GAL4^Fmrf.PS, with 10 out of 26 nervous systems examined showing complete pruning. "Hyper-pruning" beyond the normal range is also seen in these animals.

(Schubiger et al., 2003)

EcR^B2.Scer\UAS when driven by Scer\GAL4^Tab2-201Y rescues the Mushroom body γ neuron phenotypes.

(Lee et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

6469

w^*; P{UAS-EcR.B1}3b

64303

y¹ w^*; ftz-f1^ex7 P{FRT(w^hs)}2A P{UAS-EcR.B1}3b/TM3, Sb¹