FlyBase Allele Report: Dmel\shg[Ubi-p63E.sgGFP]

General Information

Symbol

Dmel\shg^{Ubi-p63E.sgGFP}

Species

D. melanogaster

Name

FlyBase ID

FBal0122908

Feature type

allele

Associated gene

Dmel\shg

Associated Insertion(s)

Carried in Construct

P{Ubi-p63E-shg.GFP}

Also Known As

ubi-DE-cad-GFP, E-cadherin-GFP, Ubi-DE-cadherin-GFP, DE-cad::GFP, ubi-ECad::GFP, ubi-E-cad::GFP, ubiDE-cadherinGFP, ubiquitin-E-CadherinGFP, E-Cad:GFP, GFP-DE-cadherin

Key Links

Transgenic product class

wild_type

(Oda and Tsukita, 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Oda and Tsukita, 2001)

Mutagen

in vitro construct

(Oda and Tsukita, 2001)

Progenitor genotype

Carried in construct

P{Ubi-p63E-shg.GFP}

Cytology

Description

Ubi-p63E promoter sequences drive expression of shg tagged at the C-terminal end with sgGFP.

(Oda and Tsukita, 2001)

Allele components

Component

Use(s)

Regulatory region(s)

Ubi-p63E

Encoded product / tool

shg

Tagged with

sgGFP

green fluorescent protein

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal wound healing, with Scer\GAL4^{VP16.mat.αTub67C}

(Hunter et al., 2015)

wild-type

(Oda and Tsukita, 2001)

Phenotype Manifest In

egg

(Oda and Tsukita, 2001)

embryonic epidermis, with Scer\GAL4^{VP16.mat.αTub67C}

(Hunter et al., 2015)

histoblast | somatic clone

(Ninov et al., 2010)

histoblast nest | somatic clone

(Ninov et al., 2010)

Detailed Description

Statement

Reference

Overexpression of shg^{Ubi-p63E.T:Avic\GFP-rs} in the embryonic epidermis under the control of Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} results in delayed wound closure compared with wild-type.

(Hunter et al., 2015)

shg^{Ubi-p63E.T:Avic\GFP-rs} clones generated in the histoblasts are largely excluded from the expanding front of the histoblast nest, and are observed remaining in the center of the nest.

(Ninov et al., 2010)

Expression of shg^{Ubi-p63E.T:Avic\GFP-rs} results in flies laying about 50% unfertilised eggs in some transgenic lines.

(Oda and Tsukita, 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhancer of

Statement

Reference

shg^{Ubi-p63E.sgGFP}, Scer\GAL4^pnr.PU is a non-enhancer of abnormal cell migration | pupal stage | somatic clone - tissue specific phenotype of CG7379^GD12222, Scer\GAL4^pnr.PU

(Rusu et al., 2021)

Suppressor of

Statement

Reference

shg^{Ubi-p63E.sgGFP} is a suppressor | partially of abnormal cytokinesis | male phenotype of Scer\GAL4^VP16.bam, scra^GD9720

(Goldbach et al., 2010)

NOT Suppressor of

Statement

Reference

shg^{Ubi-p63E.sgGFP}, Scer\GAL4^pnr.PU is a non-suppressor of abnormal cell migration | pupal stage | somatic clone - tissue specific phenotype of CG7379^GD12222, Scer\GAL4^pnr.PU

(Rusu et al., 2021)

Phenotype Manifest In

NOT Enhancer of

Statement

Reference

shg^{Ubi-p63E.sgGFP}, Scer\GAL4^pnr.PU is a non-enhancer of epithelial cell | pupal stage | somatic clone - tissue specific phenotype of CG7379^GD12222, Scer\GAL4^pnr.PU

(Rusu et al., 2021)

shg^{Ubi-p63E.sgGFP}, Scer\GAL4^pnr.PU is a non-enhancer of pupal thorax | somatic clone - tissue specific phenotype of CG7379^GD12222, Scer\GAL4^pnr.PU

(Rusu et al., 2021)

Suppressor of

Statement

Reference

shg^{Ubi-p63E.sgGFP} is a suppressor | partially of spermatid phenotype of Scer\GAL4^VP16.bam, scra^GD9720

(Goldbach et al., 2010)

NOT Suppressor of

Statement

Reference

shg^{Ubi-p63E.sgGFP}, Scer\GAL4^pnr.PU is a non-suppressor of epithelial cell | pupal stage | somatic clone - tissue specific phenotype of CG7379^GD12222, Scer\GAL4^pnr.PU

(Rusu et al., 2021)

shg^{Ubi-p63E.sgGFP}, Scer\GAL4^pnr.PU is a non-suppressor of pupal thorax | somatic clone - tissue specific phenotype of CG7379^GD12222, Scer\GAL4^pnr.PU

(Rusu et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

shg^{Ubi-p63E.T:Avic\GFP-rs} does not significantly change the impaired epidermal cell elongation phenotype seen during dorsal closure stages in embryos expressing Rab11^{S25N.Scer\UAS} under the control of Scer\GAL4^en-e16E.

(Mateus et al., 2011)

The cytokinesis defects that are seen in the spermatids of males expressing scra^GD9720 under the control of Scer\GAL4^{bam.T:Hsim\VP16} are significantly suppressed by co-expression of shg^{Ubi-p63E.T:Avic\GFP-rs} such that 39.1% of spermatids are mononucleate, 19.3% are binucleate and 41.6% have four nuclei.

(Goldbach et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

shg^{Ubi-p63E.sgGFP} rescues shg^R69

(Haruta et al., 2010)

shg^{Ubi-p63E.sgGFP} rescues Df(2R)E2/shg²

(Oda and Tsukita, 2001)

shg^{Ubi-p63E.sgGFP} rescues Df(2R)E2/shg^E17B

(Oda and Tsukita, 2001)

shg^{Ubi-p63E.sgGFP} rescues Df(2R)E2/shg^R69

(Oda and Tsukita, 2001)

shg^{Ubi-p63E.sgGFP} rescues Df(2R)E2/shg^g317

(Oda and Tsukita, 2001)

Comments

Expression of shg^{Ubi-p63E.T:Avic\GFP-rs} rescues the lethality of shg^R69. The head, ventral epidermis and tracheal defects of shg^R69 are almost completely rescued by shg^{Ubi-p63E.T:Avic\GFP-rs}.

When shg^{Ubi-p63E.T:Avic\GFP-rs} is present, shg^R69 mutant cell clones are able to grow in size in the epithelia. These epithelia developed normally into adult tissues.

When shg^{Ubi-p63E.T:Avic\GFP-rs} is present in the background, egg chambers containing shg^R69 mutant germ-line clones show normal development.

Cellularization occurs normally in embryos mutant for both maternal and zygotic shg^R69 that carry shg^{Ubi-p63E.T:Avic\GFP-rs}. Similarly, in the presence of shg^{Ubi-p63E.T:Avic\GFP-rs}, posterior invagination occurs normally, and the ectoderm initiates extension and maintains epithelial integrity in embryos mutant for both maternal and zygotic shg^R69.

(Haruta et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

58471

w^*; P{FRT(w^hs)}G13 shg¹/CyO; P{Ubi-p63E-shg.GFP}3

Kyoto

109007

w^*; P{Ubi-p63E-shg.GFP}5

Notes on Origin