FlyBase Allele Report: Dmel\ssh[1-11]

General Information

Symbol

Dmel\ssh^1-11

Species

D. melanogaster

Name

FlyBase ID

FBal0129033

Feature type

allele

Associated gene

Dmel\ssh

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Niwa et al., 2002)

Mutagen

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | somatic clone

(Ng and Luo, 2004)

visible | somatic clone

(Ren et al., 2007)

Phenotype Manifest In

actin filament | cell autonomous | ectopic | somatic clone

(Niwa et al., 2002)

adult mushroom body & axon | somatic clone

(Ng and Luo, 2004)

antennal lobe & axon | somatic clone

(Ng and Luo, 2004)

apical junction complex & ommatidial cluster | somatic clone

(Rogers et al., 2005)

filamentous actin & ommatidial cluster | ectopic | somatic clone

(Rogers et al., 2005)

filamentous actin | somatic clone

(Ren et al., 2007)

macrochaeta & mesothoracic tergum | somatic clone

(Niwa et al., 2002)

medial lobe of adult mushroom body | somatic clone

(Ng and Luo, 2004)

mushroom body medial lobe | somatic clone

(Ng and Luo, 2004)

nucleus & ommatidial cluster | somatic clone

(Rogers et al., 2005)

optic lobe neuron & axon | somatic clone

(Ng and Luo, 2004)

vertical lobe of adult mushroom body | somatic clone

(Ng and Luo, 2004)

wing hair | somatic clone

(Ren et al., 2007, Niwa et al., 2002)

Detailed Description

Statement

Reference

Homozygous clones in the wing result in a range of wing hair phenotypes similar to that of weak to moderate flr mutant clones.

Homozygous clones in the pupal wing show an increase in the level of F-actin compared to wild type, and this increase is seen both prior to and during wing hair morphogenesis.

(Ren et al., 2007)

Ectopic disorganised filamentous actin accumulates in somatic clones of ssh^1-11 homozygous cells during ommatidial assembly. This phenotype becomes apparent after cells have left the furrow, but is not apparent in cells in the furrow. In addition, apical junction organisation, cell shape and apical localisation of the nucleus are disrupted in these clones during ommatidial assembly, but expression of photoreceptor differentiation markers is not lost. (Note- clones induced at 48-96 hours after egg laying). By 48 h after pupariation these clones collapse into basal aggregates of apoptotic cells.

(Rogers et al., 2005)

Homozygous mushroom body neuroblast clones in the adult that are generated in newly hatched larvae do not have any obvious reduction in cell numbers compared to control clones. However, these clones show axon growth or axon growth plus guidance defects. In "severe" cases, the axons fail to extend beyond the peduncle and guidance defects are also detected at the peduncle or from the dendrite region, in "strong" cases, axon growth defects are seen in the dorsal and medial branches, and in "weak" cases axon growth defects are seen in the dorsal branch. Homozygous clones that contain only α/β neurons show axon growth defects in the dorsal and medial lobes. Homozygous neuroblast clones in the optic lobe or antennal lobe contralateral projection neurons fail to cross the midline, in contrast to wild type.

(Ng and Luo, 2004)

Wing hairs made by ssh^1-11 cells (in homozygous somatic clones) are much thicker than wild-type, they are also twisted and often bifurcated at their tips. Sensory bristles within clones are also defective, they have split tips and a disrupted striation pattern. Homozygous ssh^1-11 somatic clones show an over-accumulation of F-Actin filaments.

(Niwa et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

ssh^1-11 has abnormal neuroanatomy | somatic clone phenotype, suppressible by Scer\GAL4^ey-OK107/tsr^UAS.cNa

(Ng and Luo, 2004)

ssh^1-11 has abnormal neuroanatomy | somatic clone phenotype, suppressible by Scer\GAL4^ey-OK107/tsr^S3A.UAS

(Ng and Luo, 2004)

ssh^1-11 has abnormal neuroanatomy | somatic clone phenotype, suppressible by Cdc42[+]/Cdc42⁴

(Ng and Luo, 2004)

ssh^1-11 has abnormal neuroanatomy | somatic clone phenotype, suppressible by Rac2^Δ/Rac2[+]/Rac1^J10/Rac1[+]

(Ng and Luo, 2004)

ssh^1-11 has abnormal neuroanatomy | somatic clone phenotype, suppressible by Rho1^72O/Rho1[+]

(Ng and Luo, 2004)

ssh^1-11 has abnormal neuroanatomy | somatic clone phenotype, suppressible by RhoGEF2^4.1/RhoGEF2[+]

(Ng and Luo, 2004)

NOT suppressed by

Statement

Reference

ssh^1-11 has abnormal neuroanatomy | somatic clone phenotype, non-suppressible by tsr^S3E.UAS/Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Phenotype Manifest In

Suppressed by

Statement

Reference

ssh^1-11 has adult mushroom body & axon | somatic clone phenotype, suppressible by Scer\GAL4^ey-OK107/tsr^S3A.UAS

(Ng and Luo, 2004)

ssh^1-11 has adult mushroom body & axon | somatic clone phenotype, suppressible by Scer\GAL4^ey-OK107/tsr^UAS.cNa

(Ng and Luo, 2004)

ssh^1-11 has adult mushroom body | somatic clone phenotype, suppressible by Cdc42[+]/Cdc42⁴

(Ng and Luo, 2004)

ssh^1-11 has adult mushroom body | somatic clone phenotype, suppressible by Rac2^Δ/Rac2[+]/Rac1^J10/Rac1[+]

(Ng and Luo, 2004)

ssh^1-11 has adult mushroom body | somatic clone phenotype, suppressible by Rho1^72O/Rho1[+]

(Ng and Luo, 2004)

ssh^1-11 has adult mushroom body | somatic clone phenotype, suppressible by RhoGEF2^4.1/RhoGEF2[+]

(Ng and Luo, 2004)

ssh^1-11 has antennal lobe & axon | somatic clone phenotype, suppressible by Scer\GAL4^ey-OK107/tsr^S3A.UAS

(Ng and Luo, 2004)

ssh^1-11 has antennal lobe & axon | somatic clone phenotype, suppressible by Scer\GAL4^ey-OK107/tsr^UAS.cNa

(Ng and Luo, 2004)

ssh^1-11 has optic lobe neuron & axon | somatic clone phenotype, suppressible by Scer\GAL4^ey-OK107/tsr^S3A.UAS

(Ng and Luo, 2004)

ssh^1-11 has optic lobe neuron & axon | somatic clone phenotype, suppressible by Scer\GAL4^ey-OK107/tsr^UAS.cNa

(Ng and Luo, 2004)

NOT suppressed by

Statement

Reference

ssh^1-11 has adult mushroom body & axon | somatic clone phenotype, non-suppressible by tsr^S3E.UAS/Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

ssh^1-11 has antennal lobe & axon | somatic clone phenotype, non-suppressible by tsr^S3E.UAS/Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

ssh^1-11 has optic lobe neuron & axon | somatic clone phenotype, non-suppressible by tsr^S3E.UAS/Scer\GAL4^ey-OK107

(Ng and Luo, 2004)

Suppressor of

Statement

Reference

ssh^1-11/ssh[+] is a suppressor of eye phenotype of mbt^P3

(Menzel et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype of mbt^P3 hemizygous males is suppressed by ssh^1-11/+

(Menzel et al., 2007)

The axon growth and guidance defects seen in homozygous ssh^1-11 adult mushroom body clones (which are generated as neuroblast clones in newly hatched larvae) are almost completely suppressed by co-expression of one of tsr^Scer\UAS.cNa or tsr^S3A.Scer\UAS under the control of Scer\GAL4^OK107, but are not suppressed by co-expression of tsr^S3E.Scer\UAS under the control of Scer\GAL4^OK107. The axon growth defects seen in neuroblast clones of optic lobe and antennal lobe contralateral projection neurons are partially suppressed by co-expression of one of tsr^Scer\UAS.cNa or tsr^S3A.Scer\UAS under the control of Scer\GAL4^OK107, but are not suppressed by co-expression of tsr^S3E.Scer\UAS under the control of Scer\GAL4^OK107.