FlyBase Allele Report: Dmel\mars[EP2477]

General Information

Symbol

Dmel\mars^EP2477

Species

D. melanogaster

Name

FlyBase ID

FBal0131576

Feature type

allele

Associated gene

Dmel\mars

Associated Insertion(s)

P{EP}drk^EP2477

Carried in Construct

Also Known As

mars^p, EP(2)2477

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-, FlyBase, 1996-)

Progenitor genotype

Associated Insertion(s)

P{EP}drk^EP2477

Cytology

Description

The P{EP}drk^EP2477 insertion maps within mars. The insertion is also just upstream of drk, in the right orientation to drive overexpression of drk in the presence of a Scer\GAL4 driver.

(FlyBase, 1996-)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:13,503,016..13,503,016 [-]

(Hayward et al., 2014, Li et al., 2009, Zhang et al., 2009, Tan et al., 2008, Gene Disruption Project members, 2001-, FlyBase, 1996-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal mitotic cell cycle

(Li et al., 2009)

abnormal mitotic cell cycle, with Scer\GAL4^GMR.PF

(Li et al., 2009)

female sterile

(Tan et al., 2008)

viable | temperature conditional

(Li et al., 2009)

Phenotype Manifest In

egg

(Tan et al., 2008)

egg (with mars¹)

(Tan et al., 2008)

embryo

(Tan et al., 2008)

embryo (with mars¹)

(Tan et al., 2008)

spindle

(Li et al., 2009)

Detailed Description

Statement

Reference

The hatching rate of embryos from parents homozygous for mars^EP2477 is 67%. Viability is also temperature-dependent and the hatching rate is further reduced to 39.2% at 28.5[o]C.

Gynandromorphs form approximately 0.05% of the progeny from mars^EP2477 parents.

In metaphase mars^EP2477 embryos, the length and the density of mitotic spindles is significantly shorter and less, respectively, than in controls. At anaphase and at telophase, neither the length nor the density of mitotic spindles are substantially affected, indicating that polymerisation of the mitotic spindles in mars^EP2477 embryos is delayed, but not entirely prevented. Consistent with this, progression of mitotic events in patches of nuclei, each of which contain at leat two nuclei, is obviously delayed compared to those in adjacent nuclei in 13% of mars^EP2477 embryos.

The frequencies of non-disjunction in male progeny from mars^EP2477 homozygotes at 24[o]C and 28.5[o]C were 0.6% and 1.1% respectively, which are much higher than the gynandromorphy rate in the mars parent.

mars^EP2477 overexpression in the eye disc under the control of Scer\GAL4^GMR.PF results in cells in domain I being retained at interphase and many cells in domains II and III being retained at M-phase. This should be compared to the fact that most M-phase cells appear in domain I in control eye discs.

(Li et al., 2009)

Eggs laid by mars^EP2477 and mars¹/mars^EP2477 flies show a greatly reduced ability to hatch. Sperm tails can be visualised in early-arrested embryos laid by mutant females, indicating that sterility is caused by arrest in embryogenesis after fertilization.

Approximately 51% of embryos laid by mars^EP2477 mothers fail to develop beyond embryogenesis. Many show terminal phenotypes at or shortly after gastrulation.

(Tan et al., 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

mars^EP2477 has viable | temperature conditional phenotype, enhanceable by tlk[+]/Tlk^27-9

(Li et al., 2009)

mars^EP2477 has abnormal mitotic cell cycle | temperature conditional phenotype, enhanceable by tlk[+]/Tlk^27-9

(Li et al., 2009)

mars^EP2477 has abnormal mitotic cell cycle | temperature conditional phenotype, enhanceable by tlk[+]/Tlk^Δ14

(Li et al., 2009)

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, mars^EP2477 has abnormal mitotic cell cycle phenotype, suppressible by Tlk^UAS.cCa/Scer\GAL4^GMR.PF

(Li et al., 2009)

mars¹/mars^EP2477 has lethal | embryonic stage phenotype, suppressible by tacc^{S863L.Ubi-p63E.GFP}

(Tan et al., 2008)

NOT suppressed by

Statement

Reference

mars¹/mars^EP2477 has lethal | embryonic stage phenotype, non-suppressible by tacc^Ubi-p63E.GFP

(Tan et al., 2008)

Enhancer of

Statement

Reference

mars^EP2477 is an enhancer of abnormal mitotic cell cycle | temperature conditional phenotype of Tlk^Δ14

(Li et al., 2009)

mars^EP2477 is an enhancer of abnormal mitotic cell cycle | temperature conditional phenotype of Tlk^27-9

(Li et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The hatching rate from tlk^27-9/+; mars^EP2477 females is 48%, a further reduction from the 67% for mars^EP2477 embryos, indicating that mars genetically interacts with tlk.

The frequencies of non-disjunction in male progenies from tlk^27-9/+; mars^EP2477 and tlk^Δ14/+; mars^EP2477 females at 28.5[o]C is 6.1% and 4.4%; these rates are much higher than those from mars^EP2477 (1.1%) or tlk^Δ14/+ females (0.4%). This increase in frequency of non-disjunction is also temperature dependent, since there is no substantial increases in progeny from tlk^27-9/+; mars^EP2477 and tlk^Δ14/+; mars^EP2477 females at 24[o]C when compared to mars^EP2477 or tlk^Δ14/+ females.

All tlk^Δ14/+; mars^EP2477 embryos at either metaphase or anaphase exhibit asynchrony so severe that it is impossible to distinguish what phase an embryo belongs to. Asynchronous chromosome segregation is observed in embryos where most of the nuclei are likely to be at anaphase. Despite this severe asynchrony during chromosome congression or segregation, the morphology of the mitotic spindles in tlk^Δ14/+; mars^EP2477 embryos is not significantly different from that in mars^EP2477 embryos.

Co-expression of mars^EP2477 and tlk^Scer\UAS.cCa in the eye disc under the control of Scer\GAL4^GMR.PF results in the number of M-phase cells in domain III being reduced to a level close to that of wild-type, showing that metaphase arrest induced by mars^EP2477 overexpression is suppressed by tlk^Scer\UAS.cCa overexpression.

(Li et al., 2009)

The lethality of embryos laid by mars¹/mars^EP2477 mothers is rescued by tacc^{S863L.Ubi-p63E.T:Avic\GFP} but not tacc^{Ubi-p63E.T:Avic\GFP} expression.

(Tan et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mars^EP2477 is rescued by mars^UASp.cTa

(Tan et al., 2008)

mars¹/mars^EP2477 is rescued by mars^UASp.cTa

(Tan et al., 2008)

Not rescued by

mars¹/mars^EP2477 is not rescued by mars^F839A.UASp/Scer\GAL4^arm.PS

(Tan et al., 2008)

Comments

Expression of mars^{Scer\UAS.P\T.cTa} in the female germline, under the control of Scer\GAL4^arm.PS restores embryo viability and fertility in mars¹/mars^EP2477 and mars^EP2477 mutants.

Ectopic expression of mars^{F839A.Scer\UAS.P\T} under the control of Scer\GAL4^arm.PS fails to rescue the embryonic lethality of embryos laid by mars¹/mars^EP2477 mothers.

(Tan et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

CG17064^EP2477

gkap^EP2477

mars^EP2477

mars^p

(Hayward et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (6)

Report Sections

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