FlyBase Allele Report: Scer\GAL4[en-GAL4-33]

General Information

Symbol

Scer\GAL4^en-GAL4-33

Species

S. cerevisiae

Name

FlyBase ID

FBal0152210

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{GAL4}en^GAL4-33

Carried in Construct

Also Known As

en-gal4, engrailed-Gal4

Allele class

Mutagen

gene conversion

Nature of the Allele

Allele class

Mutagen

gene conversion

(Dahmann, 2003.11.6)

Progenitor genotype

P{en-lacZ(Xho)}en^Xho25

(Dahmann, 2003.11.6)

Associated Insertion(s)

P{GAL4}en^GAL4-33

Cytology

Description

The Ecol\lacZ sequences have been replaced by Scer\GAL4 sequences by gene conversion.

(Dahmann, 2003.11.6)

Allele components

Component

Use(s)

Inserted element

P{GAL4}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

wing disc posterior compartment

(Rintelen et al., 2003)

wandering third instar larval stage

wing disc posterior compartment

(Eugster et al., 2007)

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate, with trus^GD11610

(Takada et al., 2025)

decreased body size | P-stage, with trus^GD11610

(Takada et al., 2025)

decreased occurrence of cell division | cell autonomous | larval stage, with trus^GD11610

(Takada et al., 2025)

decreased size | adult stage, with Scer\GAL4^ci.PC, trus^GD11610

(Takada et al., 2025)

decreased size | adult stage, with trus^GD11610

(Takada et al., 2025)

female sterile, with trus^4-15/Df(3R)BSC847, Scer\GAL4^ci.PC, trus^UAS.cTa

(Takada et al., 2025)

lethal - all die before end of P-stage, with trus^GD11610

(Takada et al., 2025)

some die during embryonic stage, with trus^GD11610

(Takada et al., 2025)

some die during P-stage, with trus^GD11610

(Takada et al., 2025)

visible, with hh^UAS.cUa

(Eugster et al., 2007)

visible, with Hsap\DUSP1^UAS.cRa

(Rintelen et al., 2003)

visible, with Mkp3^UAS.cRa

(Rintelen et al., 2003)

visible, with Rnor\Dusp6^UAS.cRa

(Rintelen et al., 2003)

visible | adult stage, with Scer\GAL4^ci.PC, trus^GD11610

(Takada et al., 2025)

visible | adult stage, with trus^GD11610

(Takada et al., 2025)

visible | cell non-autonomous, with dally^{Δgpi.UAS.GFP}

(Eugster et al., 2007)

Phenotype Manifest In

egg | absent, with trus^4-15/Df(3R)BSC847, Scer\GAL4^ci.PC, trus^UAS.cTa

(Takada et al., 2025)

egg chamber, with trus^4-15/Df(3R)BSC847, Scer\GAL4^ci.PC, trus^UAS.cTa

(Takada et al., 2025)

follicle cell, with trus^4-15/Df(3R)BSC847, Scer\GAL4^ci.PC, trus^UAS.cTa

(Takada et al., 2025)

leg disc | larval stage, with trus^GD11610

(Takada et al., 2025)

nucleus | oogenesis, with trus^4-15/Df(3R)BSC847, Scer\GAL4^ci.PC, trus^UAS.cTa

(Takada et al., 2025)

nurse cell, with trus^4-15/Df(3R)BSC847, Scer\GAL4^ci.PC, trus^UAS.cTa

(Takada et al., 2025)

wing, with hh^UAS.cUa

(Eugster et al., 2007)

wing, with Hsap\DUSP1^UAS.cRa

(Rintelen et al., 2003)

wing, with Rnor\Dusp6^UAS.cRa

(Rintelen et al., 2003)

wing, with Scer\GAL4^ci.PC, trus^GD11610

(Takada et al., 2025)

wing, with trus^GD11610

(Takada et al., 2025)

wing blade anterior compartment | cell non-autonomous, with dally^{Δgpi.UAS.GFP}

(Eugster et al., 2007)

wing disc posterior compartment | cell autonomous | larval stage, with trus^GD11610

(Takada et al., 2025)

wing vein, with Mkp3^UAS.cRa

(Rintelen et al., 2003)

wing vein, with Scer\GAL4^ci.PC, trus^GD11610

(Takada et al., 2025)

wing vein, with trus^GD11610

(Takada et al., 2025)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT Suppressor of

Statement

Reference

Scer\GAL4^en-GAL4-33/Mkp3^UAS.cRa is a non-suppressor of wing vein | increased number phenotype of rl^Sem

(Rintelen et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^ci.PC, trus^UAS.cTa, Scer\GAL4^en-GAL4-33 rescues trus^4-15/Df(3R)BSC847

(Takada et al., 2025)

dally^UAS.GFP/Scer\GAL4^en-GAL4-33 rescues dally⁸⁰

(Eugster et al., 2007)

Partially rescues

trus^UAS.cTa/Scer\GAL4^en-GAL4-33 partially rescues trus^4-15/Df(3R)BSC847

(Takada et al., 2025)

dally^{Δgpi.UAS.GFP}/Scer\GAL4^en-GAL4-33 partially rescues dally⁸⁰

(Eugster et al., 2007)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

99568

y¹ w^*; P{GAL4}en^GAL4-33

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Scer\GAL4^en-GAL4-33

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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