Compared to wild type (pupate around day 5, eclose around day 10), Tcs3f01978/Df(3L)ED220 exhibit an extended larval period prior to lethality (viability: 88% at day 8, 15% at day 16) and have melanotic masses ('black spots': 16% at day 8, 30% at day 16); larvae are small and unable to dig into food.
Compared to control Tcs3f01978/+ heterozygotes, Tcs3f01978/Df(3L)ED220 larval hemolymph has an overrepresentation of lamellocytes and second instar larvae have overgrown anterior lymph gland lobes.
Most Tcs3f01978/Df(3L)ED220 third instar larvae are missing imaginal discs and have a slightly smaller CNS; optic lobes are strongly reduced in size, while the ventral nerve cord is less affected.
Tcs3f01978/Df(3L)ED220 have significantly smaller salivary glands, salivary gland cell size, and body wall musculature (length of ventral longitudinal muscles 1 and 2) compared to both Tcs31A13/Df(3L)ED220 and wild type larvae.
Homozygous Tcs3f01978 mutant larvae are smaller than wild-type animals. The mutants do not pupariate and die at larval stages.