FlyBase Allele Report: Dmel\Tcs3[f01978]

General Information

Symbol

Dmel\Tcs3^f01978

Species

D. melanogaster

Name

FlyBase ID

FBal0161348

Feature type

allele

Associated gene

Dmel\Tcs3

Associated Insertion(s)

PBac{WH}Tcs3^f01978

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

piggyBac activity

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Lin et al., 2015, Rojas-Benitez et al., 2015)

Mutagen

piggyBac activity

(Bloomington Drosophila Stock Center, 2004.11.6)

Progenitor genotype

Associated Insertion(s)

PBac{WH}Tcs3^f01978

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:16,352,048..16,352,048 [+]

(Rojas-Benitez et al., 2015, Gene Disruption Project members and Exelixis, 2005, Bloomington Drosophila Stock Center, 2004.11.6)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal developmental rate | larval stage (with Df(3L)ED220)

(Lin et al., 2015)

abnormal neuroanatomy | third instar larval stage (with Df(3L)ED220)

(Lin et al., 2015)

decreased body size | larval stage (with Df(3L)ED220)

(Lin et al., 2015)

decreased body size | third instar larval stage

(Rojas-Benitez et al., 2015)

decreased body size | third instar larval stage (with Tcs3^e01173)

(Rojas-Benitez et al., 2015)

decreased cell size | larval stage (with Df(3L)ED220)

(Lin et al., 2015)

hypoplasia | third instar larval stage (with Df(3L)ED220)

(Lin et al., 2015)

lethal - all die before end of larval stage

(Rojas-Benitez et al., 2015)

lethal - all die before end of P-stage (with Df(3L)ED220)

(Lin et al., 2015)

melanotic mass phenotype (with Df(3L)ED220)

(Lin et al., 2015)

neoplasia | larval stage (with Df(3L)ED220)

(Lin et al., 2015)

Phenotype Manifest In

Detailed Description

Statement

Reference

Compared to wild type (pupate around day 5, eclose around day 10), Tcs3^f01978/Df(3L)ED220 exhibit an extended larval period prior to lethality (viability: 88% at day 8, 15% at day 16) and have melanotic masses ('black spots': 16% at day 8, 30% at day 16); larvae are small and unable to dig into food.

Compared to control Tcs3^f01978/+ heterozygotes, Tcs3^f01978/Df(3L)ED220 larval hemolymph has an overrepresentation of lamellocytes and second instar larvae have overgrown anterior lymph gland lobes.

Most Tcs3^f01978/Df(3L)ED220 third instar larvae are missing imaginal discs and have a slightly smaller CNS; optic lobes are strongly reduced in size, while the ventral nerve cord is less affected.

Tcs3^f01978/Df(3L)ED220 have significantly smaller salivary glands, salivary gland cell size, and body wall musculature (length of ventral longitudinal muscles 1 and 2) compared to both Tcs3^1A13/Df(3L)ED220 and wild type larvae.

(Lin et al., 2015)

Homozygous Tcs3^f01978 mutant larvae are smaller than wild-type animals. The mutants do not pupariate and die at larval stages.

(Rojas-Benitez et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement