FlyBase Allele Report: Dmel\upd3[RNAi.UAS]

General Information

Symbol

Dmel\upd3^RNAi.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0177288

Feature type

allele

Associated gene

Dmel\upd3

Associated Insertion(s)

Carried in Construct

P{UAS-upd3.RNAi}

Also Known As

UAS-Upd3-IR, UAS-iupd3, upd3-IR

Key Links

Transgenic product class

RNAi_reagent

(Agaisse et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Agaisse et al., 2003)

Mutagen

in vitro construct

(Agaisse et al., 2003)

Progenitor genotype

Carried in construct

P{UAS-upd3.RNAi}

Cytology

Description

An inverted repeat of a 500bp fragment from upd3 cDNA, separated by a sequence corresponding to the 74bp second intron of the w gene, is under the control of UAS sequences.

(Agaisse et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

upd3

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal immune response | adult stage, with Scer\GAL4^Myo31DF.PU

(Zhou et al., 2013)

abnormal immune response | adult stage, with Scer\GAL4^Su(H).GBE

(Zhou et al., 2013)

increased cell number | larval stage, with Scer\GAL4^Hand.Δ

(Destalminil-Letourneau et al., 2021)

increased cell number | larval stage, with Scer\GAL4^NP1029

(Destalminil-Letourneau et al., 2021)

Phenotype Manifest In

embryonic/larval crystal cell | increased number | larval stage, with Scer\GAL4^Hand.Δ

(Destalminil-Letourneau et al., 2021)

embryonic/larval crystal cell | increased number | larval stage, with Scer\GAL4^NP1029

(Destalminil-Letourneau et al., 2021)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^Myo31DF.PU

(Zhou et al., 2013)

intestinal stem cell of posterior adult midgut epithelium, with Scer\GAL4^Su(H).GBE

(Zhou et al., 2013)

Detailed Description

Statement

Reference

Expression of upd3^{dsRNA.Scer\UAS} under the control of either Scer\GAL4^Myo31DF.PU or Scer\GAL4^Su(H).GBE reduces the increase in the rate of intestinal stem cell division observed upon bacteria infection in the adult midgut by about 50% compared to wild-type.

(Zhou et al., 2013)

Expression of upd3^{dsRNA.Scer\UAS} under the control of Scer\GAL4^tub.PU and Scer\GAL4^esg-NP7397 in the intestinal stem cells (ISCs) and their subsequent lineage (using the "esg[F/O]" flip-out lineage system to mark ISC progenitors and their subsequent lineage) has no effect on epithelium turnover or ISC maintenance in the midgut.

(Osman et al., 2012)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^Su(H).GBE/upd3^RNAi.UAS is a suppressor | partially of neoplasia phenotype of Sox21a⁶

(Zhai et al., 2015)

NOT Suppressor of

Statement

Reference

upd3^RNAi.UAS, Scer\GAL4^Antp-10 is a non-suppressor of increased cell number | third instar larval stage phenotype of ND-75^GD6309, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^Su(H).GBE/upd3^RNAi.UAS is a suppressor | partially of adult anterior midgut epithelium phenotype of Sox21a⁶

(Zhai et al., 2015)

NOT Suppressor of

Statement

Reference

upd3^RNAi.UAS, Scer\GAL4^Antp-10 is a non-suppressor of lamellocyte | third instar larval stage phenotype of ND-75^GD6309, Scer\GAL4^Antp-10

(Sinenko et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of upd3^{dsRNA.Scer\UAS} in enteroblasts under the control of Scer\GAL4^Su(H).GBE reduces tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

(Zhai et al., 2015)

Expression of upd3^{dsRNA.Scer\UAS} does not suppress the increase in circulating lamellocytes seen when ND75^GD6309 is expressed under the control of Scer\GAL4^Antp-10.

(Sinenko et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in plasmid "pWIZ" and transfected into flies to study the consequences of dsRNA interference (RNAi) of the upd3 gene in specific tissues on expression of TotA.

(Agaisse et al., 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

upd3^RNAi.UAS

upd3^{dsRNA.Scer\UAS}

upd3^dsRNA.UAS

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

Open Close