loss of function allele
abnormal feeding behavior, with Scer\GAL4Gr66a.PD, mttdsRNA.UAS.RNAi
abnormal feeding behavior, with Scer\GAL4NP4288, mttdsRNA.UAS.RNAi
abnormal feeding behavior | adult stage (with Df(2R)Exel7096)
abnormal feeding behavior | adult stage (with mttdsRNA.UAS.RNAi), with Scer\GAL4Gr66a.PD
abnormal feeding behavior | adult stage (with mttdsRNA.UAS.RNAi), with Scer\GAL4NP4288
abnormal feeding behavior | adult stage | recessive
viable (with Df(2R)Exel7096)
The electrophysiological responses of the S3, S5 and S10 taste sensilla to L-canavanine are normal in both mttf06268 and mttf06268/Df(2R)Exel7096 flies.
mttf06268 flies show normal behavioural avoidance to L-canavanine in a two-way choice test.
mttf06268/mttf06268 and mttf06268/Df(2R)Exel7096 flies feed on a solution containing 30 mM L-canavanine (an insecticide), whereas control flies are repulsed by L-canavanine and avoid feeding on it.
L-canavanine triggers proboscis retraction in wild type flies but not in mttf06268 homozygous or mttf06268/Df(2R)Exel7096 flies.
Expression of mttdsRNA.Scer\UAS.RNAi using either Scer\GAL4NP4288 or Scer\GAL4Gr66a.PD in a mttf06268/+ background abolishes the L-canavanine-induced proboscis retraction seen in wild type flies.
mttf06268 is rescued by mttUAS.Tag:HA/Scer\GAL4Gr66a.PD
mttf06268 is not rescued by mttUAS.Tag:HA/Scer\GAL4NP1017
mttf06268 is not rescued by mttUAS.Tag:HA/Scer\GAL4Gr5a.8.5
Expression of mttScer\UAS.T:Ivir\HA1 using Scer\GAL4Gr66a.PD re-establishes L-canavanine-induced proboscis retraction in mttf06268 flies. In contrast, expressing mttScer\UAS.T:Ivir\HA1 using Scer\GAL4NP1017 or Scer\GAL4Tre.8.5.cCa does not rescue the mttf06268 phenotype.