FlyBase Allele Report: Dmel\mtt[f06268]

General Information

Symbol

Dmel\mtt^f06268

Species

D. melanogaster

Name

FlyBase ID

FBal0178962

Feature type

allele

Associated gene

Dmel\mtt

Associated Insertion(s)

PBac{WH}mtt^f06268

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

piggyBac activity

Nature of the Allele

Allele class

loss of function allele

(Mitri et al., 2009)

Mutagen

piggyBac activity

(Gene Disruption Project members and Exelixis, 2005)

Progenitor genotype

Associated Insertion(s)

PBac{WH}mtt^f06268

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{WH}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:8,483,275..8,483,275 [+]

(Mitri et al., 2009, Gene Disruption Project members and Exelixis, 2005)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

The electrophysiological responses of the S3, S5 and S10 taste sensilla to L-canavanine are normal in both mtt^f06268 and mtt^f06268/Df(2R)Exel7096 flies.

mtt^f06268 flies show normal behavioural avoidance to L-canavanine in a two-way choice test.

(Lee et al., 2012)

mtt^f06268/mtt^f06268 and mtt^f06268/Df(2R)Exel7096 flies feed on a solution containing 30 mM L-canavanine (an insecticide), whereas control flies are repulsed by L-canavanine and avoid feeding on it.

L-canavanine triggers proboscis retraction in wild type flies but not in mtt^f06268 homozygous or mtt^f06268/Df(2R)Exel7096 flies.

Expression of mtt^{dsRNA.Scer\UAS.RNAi} using either Scer\GAL4^NP4288 or Scer\GAL4^Gr66a.PD in a mtt^f06268/+ background abolishes the L-canavanine-induced proboscis retraction seen in wild type flies.

(Mitri et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

mtt^f06268 is rescued by mtt^UAS.Tag:HA/Scer\GAL4^Gr66a.PD

(Mitri et al., 2009)

Not rescued by

mtt^f06268 is not rescued by mtt^UAS.Tag:HA/Scer\GAL4^NP1017

(Mitri et al., 2009)

mtt^f06268 is not rescued by mtt^UAS.Tag:HA/Scer\GAL4^Gr5a.8.5

(Mitri et al., 2009)

Comments

Expression of mtt^{Scer\UAS.T:Ivir\HA1} using Scer\GAL4^Gr66a.PD re-establishes L-canavanine-induced proboscis retraction in mtt^f06268 flies. In contrast, expressing mtt^{Scer\UAS.T:Ivir\HA1} using Scer\GAL4^NP1017 or Scer\GAL4^Tre.8.5.cCa does not rescue the mtt^f06268 phenotype.

(Mitri et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

mXr^f06268

mtt^f06268

(Lee et al., 2012, Mitri et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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