FlyBase Allele Report: Dmel\Gyc76C[2388]

General Information

Symbol

Dmel\Gyc76C²³⁸⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0190851

Feature type

allele

Associated gene

Dmel\Gyc76C

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Patel et al., 2012)

Mutagen

ethyl methanesulfonate

(Myat et al., 2005)

Progenitor genotype

Cytology

Description

T to G change at the 5' end of the intron following exon 16, changing the conserved GU at the splice donor site into GG. This results in the insertion of 92bp of sequence from intron 6, which contains an in-frame stop codon before the guanylyl cyclase domain.

(Patel et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration

(Patel and Myat, 2013)

abnormal cell migration (with Gyc76C^KG03723ex173)

(Patel and Myat, 2013)

lethal (with Df(3L)XS533)

(Patel et al., 2012)

Phenotype Manifest In

Detailed Description

Statement

Reference

In Gyc76C²³⁸⁸ heterozygous embryos, all salivary gland cells invaginate to form a gland that turns posteriorly during stages 13 and 14. By contrast, in Gyc76C²³⁸⁸ homozygous embryos, 16% of mutant glands do not invaginate completely, with some cells remaining at the ventral surface. Gyc76C²³⁸⁸ homozygous embryos display a severe salivary gland migration defect where 88% of mutant glands fail to turn completely and some glands appear branched or folded.

In Gyc76C²³⁸⁸ heterozygous embryos, the salivary gland lumen is a single continuous structure formed during the process of invagination. By contrast, in Gyc76C²³⁸⁸ homozygous embryos, the salivary gland lumen is branched with ectopic lumens along the length of the central lumen. Branching of the salivary gland lumen in Gyc76C²³⁸⁸ mutant embryos occurs through indentation of the apical membrane during invagination or through formation of an ectopic invagination site.

Gyc76C^KG03723ex173/Gyc76C²³⁸⁸ heterozygous embryos display a severe salivary gland migration defect where mutant gland fail to turn completely and some glands appear branched or folded.

(Patel and Myat, 2013)

Homozygous embryos show defects in the somatic muscle pattern. Numerous ventral and lateral muscle fail to extend or are missing. Free, unfused fusion competent myoblasts are present in some of the mutant embryos. Dorsal muscle patterning is largely unaffected. 60% of homozygous embryos show muscle defects at stage 15, but this is reduced to 20% at stage 16, indicating delayed muscle development.

Patterning of the ventral and lateral somatic muscles is abnormal in Gyc76C^KG03723ex173/Gyc76C²³⁸⁸ embryos, whereas dorsal muscles are patterned normally.

Patterning of the ventral and lateral somatic muscles is abnormal in Gyc76C^KG03723ex173/Df(3L)XS533 embryos.

(Patel et al., 2012)

In gim²³⁸⁸ embryos, the tracheal branches fail to migrate in a wild-type pattern; the dorsal trunk and visceral branches are the most severely affected.

(Myat et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Gyc76C²³⁸⁸ has abnormal cell migration phenotype, enhanceable by LanB2^MB04039

(Patel and Myat, 2013)

Enhancer of

Statement

Reference

Gyc76C²³⁸⁸ is an enhancer of abnormal cell migration phenotype of LanB2^MB04039

(Patel and Myat, 2013)

Phenotype Manifest In

Enhanced by

Statement

Reference

Gyc76C²³⁸⁸ has embryonic/larval salivary gland phenotype, enhanceable by LanB2^MB04039

(Patel and Myat, 2013)

Enhancer of

Statement

Reference

Gyc76C²³⁸⁸ is an enhancer of embryonic/larval salivary gland phenotype of LanB2^MB04039

(Patel and Myat, 2013)

Additional Comments

Genetic Interactions

Statement

Reference

82% of salivary glands LanB2^MB04039 Gyc76C²³⁸⁸ transheterozygous embryos do not migrate completely.

LanB2^MB04039 Gyc76C²³⁸⁸ double mutants exacerbate the salivary gland invagination defect such that 25% of mutant glands fail to invaginate compared to 16% in Gyc76C²³⁸⁸ and 13% in LanB2^MB04039 single mutant glands.

(Patel and Myat, 2013)

Gyc76C²³⁸⁸/Df(3R)e-19 transheterozygotes do not show defects in salivary gland development.

(Patel et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Gyc76C²³⁸⁸ is rescued by Scer\GAL4^fkh.PH/Gyc76C^UAS.Tag:MYC

(Patel and Myat, 2013)

Gyc76C²³⁸⁸ is rescued by Gyc76C^UAS.Tag:MYC/Scer\GAL4^twi.PB

(Patel and Myat, 2013)

Gyc76C²³⁸⁸ is rescued by Scer\GAL4^fkh.PH/Gyc76C^UAS.Tag:MYC/Scer\GAL4^twi.PB

(Patel and Myat, 2013)

Partially rescued by

Gyc76C²³⁸⁸ is partially rescued by Gyc76C^UAS.Tag:MYC/Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR

(Patel et al., 2012)

Comments

Expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} in either the salivary glands or mesoderm, under the control of Scer\GAL4^fkh.PH or Scer\GAL4^twi.PB significantly rescues the gland migration defect found in Gyc76C²³⁸⁸ homozygous embryos. Simultaneous expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} in the salivary gland and mesoderm does not result in increased rescue of the migration defect compared to expression in either tissue alone. In contrast to the rescue of the migration defects, the salivary gland invagination defects of Gyc76C²³⁸⁸ mutant embryos are better rescued by expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} in the gland than in the mesoderm.

(Patel and Myat, 2013)

Expression of Gyc76C^{Scer\UAS.T:Hsap\MYC} under the simultaneous control of both Scer\GAL4^Mef2.PR and Scer\GAL4^twi.PB partially rescues the somatic muscle defects of Gyc76C²³⁸⁸ homozygous embryos, such that only 10% of stage 14 and 5% of stage 16 embryos show a patterning defect.

(Patel et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a mutation that disrupts tracheal development when homozygous.

(Myat et al., 2005)

The mutant "gim[2388]" chromosome originally contained two separable lethal mutations: one at 76B-77B (corresponding to a mutation in Gyc76C) which results in salivary gland defects, and one at 93B-93D (uncovered by Df(3R)e-19) which does not result in in salivary gland defects.

(Patel et al., 2012)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Gyc76C²³⁸⁸

(Patel and Myat, 2013)

gim²³⁸⁸

gimli²³⁸⁸

(Patel et al., 2012)

gyc76C²³⁸⁸

(Patel et al., 2012)

Name Synonyms

Secondary FlyBase IDs

References (3)

Report Sections

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