FlyBase Allele Report: Dmel\KaiR1D[MB01010]

General Information

Symbol

Dmel\KaiR1D^MB01010

Species

D. melanogaster

Name

FlyBase ID

FBal0192503

Feature type

allele

Associated gene

Dmel\KaiR1D

Associated Insertion(s)

Mi{ET1}KaiR1D^MB01010

Carried in Construct

Also Known As

DKaiR1D²

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Kiragasi et al., 2017)

Mutagen

Progenitor genotype

Associated Insertion(s)

Mi{ET1}KaiR1D^MB01010

Cytology

Description

Allele components

Component

Use(s)

Inserted element

Mi{ET1}

enhancer trap

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3R:20,863,213..20,863,213 [+]

(Kiragasi et al., 2017, Bellen, 2006.10.10)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | larval stage

(Kiragasi et al., 2017)

abnormal neurophysiology | larval stage

(Kiragasi et al., 2017)

abnormal neurophysiology | third instar larval stage

(Kiragasi et al., 2020)

Phenotype Manifest In

embryonic/larval neuromuscular junction | larval stage

(Kiragasi et al., 2017)

embryonic/larval neuromuscular junction | third instar larval stage

(Kiragasi et al., 2020)

muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage

(Kiragasi et al., 2020)

Detailed Description

Statement

Reference

KaiR1D^MB01010 third instar larval NMJs exhibit reduced excitatory postsynaptic current (EPSC) amplitudes and quantal contents.

(Kiragasi et al., 2020)

Neurotransmission at KaiR1D^MB01010 homozygous larval neuromuscular junctions display significant decreases in evoked excitatory postsynaptic potentials and in quantal contents under low extracellular Ca[2+] concentration conditions (0.2-0.3mM), but not under 0.4mM extracellular Ca[2+] concentration conditions, as compared to controls; miniature excitatory postsynaptic potential do not seem significantly altered. Under 0.2mM concentrations, mutant larval neuromuscular junctions display increased short-term facilitation, as shown by the significant increase in evoked excitatory postsynaptic potentials under 20Hz stimulation, as compared to controls. Under 3mM extracellular Ca[2+] concentration conditions, these mutants do not display significant changes in excitatory postsynaptic current amplitude or in readily releasable pool, as compared to controls. These neuromuscular junctions do not display significant changes in the number of synaptic boutons, in the number, density or length of presynaptic active zones, or in the density of synaptic vesicles, as compared to controls.

KaiR1D^MB01010 homozygous larval motor neurons under extracellular Ca[2+] concentration conditions virtually do not respond to glutamate photo uncaging, as compared to controls.

(Kiragasi et al., 2017)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

KaiR1D^MB01010, Scer\GAL4^VGlut1-OK371 has abnormal neurophysiology | larval stage phenotype, enhanceable by VGlut1^UAS.cDa, Scer\GAL4^VGlut1-OK371

(Kiragasi et al., 2017)

NOT Enhanced by

Statement

Reference

KaiR1D^MB01010 has abnormal neurophysiology | third instar larval stage phenotype, non-enhanceable by Sol1^bk1

(Kiragasi et al., 2020)

NOT suppressed by

Statement

Reference

KaiR1D^MB01010 has abnormal neurophysiology | third instar larval stage phenotype, non-suppressible by Sol1^bk1

(Kiragasi et al., 2020)

Enhancer of

Statement

Reference

KaiR1D^MB01010/KaiR1D^MB01010 is an enhancer of abnormal neurophysiology | larval stage phenotype of Scer\GAL4^VGlut1-OK371, VGlut1^UAS.cDa

(Kiragasi et al., 2017)

NOT Enhancer of

Statement

Reference

KaiR1D^MB01010 is a non-enhancer of abnormal neurophysiology | third instar larval stage phenotype of Sol1^bk1

(Kiragasi et al., 2020)

Suppressor of

Statement

Reference

KaiR1D^MB01010 is a suppressor of abnormal neurophysiology | third instar larval stage phenotype of Scer\GAL4^RapGAP1-OK6, Sol1^UAS.cKa

(Kiragasi et al., 2020)

KaiR1D^MB01010/KaiR1D^MB01010 is a suppressor of abnormal neurophysiology | larval stage phenotype of GluRIIA^SP16

(Kiragasi et al., 2017)

NOT Suppressor of

Statement

Reference

KaiR1D^MB01010 is a non-suppressor of abnormal neurophysiology | third instar larval stage phenotype of Sol1^bk1

(Kiragasi et al., 2020)

Scer\GAL4^RapGAP1-OK6/KaiR1D^UAS.cKa, KaiR1D^MB01010/KaiR1D^MB01010 is a non-suppressor of abnormal neurophysiology | larval stage phenotype of GluRIIA^SP16

(Kiragasi et al., 2017)

Phenotype Manifest In

Enhanced by

Statement

Reference

KaiR1D^MB01010, Scer\GAL4^VGlut1-OK371 has embryonic/larval neuromuscular junction | larval stage phenotype, enhanceable by VGlut1^UAS.cDa, Scer\GAL4^VGlut1-OK371

(Kiragasi et al., 2017)

NOT Enhanced by

Statement

Reference

KaiR1D^MB01010 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-enhanceable by Sol1^bk1

(Kiragasi et al., 2020)

KaiR1D^MB01010 has muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage phenotype, non-enhanceable by Sol1^bk1

(Kiragasi et al., 2020)

NOT suppressed by

Statement

Reference

KaiR1D^MB01010 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Sol1^bk1

(Kiragasi et al., 2020)

KaiR1D^MB01010 has muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage phenotype, non-suppressible by Sol1^bk1

(Kiragasi et al., 2020)

Enhancer of

Statement

Reference

KaiR1D^MB01010/KaiR1D^MB01010 is an enhancer of embryonic/larval neuromuscular junction | larval stage phenotype of Scer\GAL4^VGlut1-OK371, VGlut1^UAS.cDa

(Kiragasi et al., 2017)

NOT Enhancer of

Statement

Reference

KaiR1D^MB01010 is a non-enhancer of muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage phenotype of Sol1^bk1

(Kiragasi et al., 2020)

KaiR1D^MB01010 is a non-enhancer of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Sol1^bk1

(Kiragasi et al., 2020)

Suppressor of

Statement

Reference

KaiR1D^MB01010 is a suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Scer\GAL4^RapGAP1-OK6, Sol1^UAS.cKa

(Kiragasi et al., 2020)

KaiR1D^MB01010 is a suppressor of muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage phenotype of Scer\GAL4^RapGAP1-OK6, Sol1^UAS.cKa

(Kiragasi et al., 2020)

KaiR1D^MB01010/KaiR1D^MB01010 is a suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of GluRIIA^SP16

(Kiragasi et al., 2017)

NOT Suppressor of

Statement

Reference

KaiR1D^MB01010 is a non-suppressor of embryonic/larval neuromuscular junction | third instar larval stage phenotype of Sol1^bk1

(Kiragasi et al., 2020)

KaiR1D^MB01010 is a non-suppressor of muscle cell of A1-7 ventral longitudinal muscle 3 | third instar larval stage phenotype of Sol1^bk1

(Kiragasi et al., 2020)

Scer\GAL4^RapGAP1-OK6/KaiR1D^UAS.cKa, KaiR1D^MB01010/KaiR1D^MB01010 is a non-suppressor of embryonic/larval neuromuscular junction | larval stage phenotype of GluRIIA^SP16

(Kiragasi et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

KaiR1D^MB01010 homozygosity alone, but not in combination with the expression of KaiR1D^Scer\UAS.cKa under the control of Scer\GAL4^RapGAP1-OK6, suppresses the increased quantal content, but not the decreased miniature excitatory postsynaptic potentials, observed at the larval neuromuscular junctions of GluRIIA^SP16 homozygotes under 0.4mM extracellular Ca[2+] concentration conditions. KaiR1D^MB01010 homozygosity, however, does not suppress the decreased excitatory postsynaptic current amplitude or the increased readily releasable pool observed at the larval neuromuscular junctions of GluRIIA^SP16 homozygotes under 3mM extracellular Ca[2+] concentration conditions.

The decreased evoked excitatory postsynaptic potentials and quantal contents under 0.2mM extracellular Ca[2+] concentration conditions observed at the larval neuromuscular junctions of individuals either homozygous for KaiR1D^MB01010 or expressing VGlut^Scer\UAS.cDa under the control of Scer\GAL4^VGlut-OK371 are enhanced in double mutants. KaiR1D^MB01010 homozygosity, however, does not significantly affect the increased miniature excitatory postsynaptic potentials and the reciprocal decreased quantal contents under 0.4mM extracellular Ca[2+] concentration conditions observed at the larval neuromuscular junctions of individuals expressing VGlut^Scer\UAS.cDa under the control of Scer\GAL4^VGlut-OK371.

(Kiragasi et al., 2017)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

KaiR1D^MB01010 is rescued by Scer\GAL4^RapGAP1-OK6/KaiR1D^UAS.cKa

(Kiragasi et al., 2017)

Not rescued by

KaiR1D^MB01010 is not rescued by KaiR1D^Q604R.UAS/Scer\GAL4^RapGAP1-OK6

(Kiragasi et al., 2017)

Comments

The decreased evoked excitatory postsynaptic potentials and quantal contents under 0.2mM extracellular Ca[2+] concentration conditions observed at KaiR1D^MB01010-homozygous larval neuromuscular junctions is rescued by the expression of KaiR1D^Scer\UAS.cKa, but not KaiR1D^{Q604R.Scer\UAS}, under the control of Scer\GAL4^RapGAP1-OK6.

(Kiragasi et al., 2017)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

22962

y¹ w^67c23; Mi{ET1}KaiR1D^MB01010

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG3822^MB01010

(Bellen, 2006.10.10)

DKaiR1D²

(Kiragasi et al., 2020, Kiragasi et al., 2017)

KaiR1D^MB01010

Name Synonyms

Secondary FlyBase IDs

References (3)

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