FlyBase Allele Report: Dmel\Arp3[GD12273]

General Information

Symbol

Dmel\Arp3^GD12273

Species

D. melanogaster

Name

FlyBase ID

FBal0198760

Feature type

allele

Associated gene

Dmel\Arp3

Associated Insertion(s)

Carried in Construct

P{GD12273}

Also Known As

UAS-arp3RNAi, UAS-Arp3 RNAi, arp3 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12273}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12273

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration, with Scer\GAL4^Pxn.PS

(Verboon et al., 2015)

abnormal locomotor behavior | third instar larval stage, with Scer\GAL4^Ubi.PU

(Koch et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^C57

(Nahm et al., 2010)

abnormal neuroanatomy | larval stage, with Scer\GAL4^Ubi.PU

(Koch et al., 2014)

abnormal neuroanatomy | larval stage, with Scer\GAL4^VGlut1-OK371

(Koch et al., 2014)

abnormal wound healing | P-stage, with Scer\GAL4^A58

(Rötte et al., 2024)

partially lethal - majority die, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

short lived | heat sensitive, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

bouton, with Scer\GAL4^C57

(Nahm et al., 2010)

cell projection, with Scer\GAL4^Pxn.PS

(Verboon et al., 2015)

embryonic/larval hemocyte, with Scer\GAL4^Pxn.PS

(Verboon et al., 2015)

embryonic/larval neuromuscular junction | larval stage, with Scer\GAL4^Ubi.PU

(Koch et al., 2014)

lamellipodium | P-stage, with Scer\GAL4^A58

(Rötte et al., 2024)

neuromuscular junction, with Scer\GAL4^C57

(Nahm et al., 2010)

synapse, with Scer\GAL4^C57

(Nahm et al., 2010)

trichogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

type Ib terminal bouton | larval stage, with Scer\GAL4^Ubi.PU

(Koch et al., 2014)

type Ib terminal bouton | larval stage, with Scer\GAL4^VGlut1-OK371

(Koch et al., 2014)

vacuole | increased number, with Scer\GAL4^Pxn.PS

(Verboon et al., 2015)

Detailed Description

Statement

Reference

Hemocyte trans-migration from the head to the tail is similar in embryos expressing Arp3^GD12273 under the control of Scer\GAL4^Pxn.PS and wild-type. However, compared with wild-type, expression of Scer\GAL4^Pxn.PS>Arp3^GD12273 significantly reduces the number of hemocytes that migrate anteriorly from the tail along the ventral midline. Scer\GAL4^Pxn.PS>Arp3^GD12273 hemocytes are also less organized resulting in the simultaneous formation of three lines of hemocytes migrating posteriorly from the head rather than the lateral movement of hemocytes from the ventral midline observed in wild-type embryos.

Compared with wild-type, posterior hemocytes in Scer\GAL4^Pxn.PS>Arp3^GD12273 embryos exhibit severe reduction in cellular protrusion area. The anterior hemocytes of the same genotype also show a moderate reduction in cellular protrusion area. The number of vacuoles present in both the anterior and posterior hemocyte cell bodies is increased relative to wild-type.

(Verboon et al., 2015)

Larvae expressing Arp3^GD12273 under the control of Scer\GAL4^Ubi have neuromuscular junction (NMJ) defects. The number of type Ib boutons per NMJ and the number of axonal branch points per NMJ are significantly reduced compared to controls.

Larvae expressing Arp3^GD12273 under the control of Scer\GAL4^VGlut-OK371 show a significant reduction in the number of type Ib boutons per NMJ compared to controls, but the number of axonal branch points per NMJ is not significantly affected.

The average speed of migration of third instar larvae expressing Arp3^GD12273 under the control of Scer\GAL4^Ubi is significantly reduced compared to wild type.

(Koch et al., 2014)

Muscle-specific expression of Arp66B^GD12273 under the control of Scer\GAL4^C57 results in synaptic overgrowth.

(Nahm et al., 2010)

Adults expressing Arp3^GD12273 under the control of the cardioblast-specific Scer\GAL4^tin.CΔ4 driver show significantly reduced survival on day 6 after a shift to 29[o]C compared to control flies.

(Neely et al., 2010)

Adults expressing Arp3^GD12273 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or late pupal lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 may result in semi-lethality, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 0% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 0% or 30-40% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 0% or 40-50% of the Scer\GAL4^pnr-MD237-expressing area of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12273

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Arp3^GD12273, Scer\GAL4^ptc.PU is a suppressor of visible | adult stage phenotype of Dora^GD6527, Scer\GAL4^ptc.PU

(Molina-Pelayo et al., 2022)

Other

Statement

Reference

Arp3^GD12273, Arpc1^GD14085, Scer\GAL4^A3.eya has male sterile phenotype

(Rotkopf et al., 2011)

Phenotype Manifest In

Suppressor of

Statement

Reference

Arp3^GD12273, Scer\GAL4^ptc.PU is a suppressor of wing hair phenotype of Dora^GD6527, Scer\GAL4^ptc.PU

(Molina-Pelayo et al., 2022)

Other

Statement

Reference

Arp3^GD12273, Arpc1^GD14085, Scer\GAL4^A3.eya has seminal vesicle phenotype

(Rotkopf et al., 2011)

Arp3^GD12273, Arpc1^GD14085, Scer\GAL4^A3.eya has spermatid cyst phenotype

(Rotkopf et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Larvae co-expressing Arp66B^GD12273 and Sop2^GD14085 under the control of Scer\GAL4^moody.PK show an increased penetration of 3kD fluorescein-dextran into the ventral nerve cord in a permeability assay compared to the dye penetration seen in control larvae.

(Hatan et al., 2011)

Co-expression of Arpc1^GD14085, Arp3^GD12273 and Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^A3.eya results in a male-sterile phenotype, characterised by spermatogenic arrest at the elongated cyst stage and empty seminal vesicles.

(Rotkopf et al., 2011)

Co-expression of Sop2^GD14085 and Arp66B^GD12273 under the control of Scer\GAL4^btl.PS in embryos does not alter apical F-actin in the trachea.

Co-expression of Sop2^GD14085 and Arp66B^GD12273 under the control of Scer\GAL4^Mef2.PR in embryos results in a muscle fusion phenotype.

(Massarwa et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

VDRC

v35258

w¹¹¹⁸; P{GD12273}v35258

v35260

w¹¹¹⁸; P{GD12273}v35260

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12273

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Arp3^GD12273

Arp66B^GD12273

Name Synonyms

Secondary FlyBase IDs

References (19)

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