FlyBase Allele Report: Dmel\Rdl[GD4609]

General Information

Symbol

Dmel\Rdl^GD4609

Species

D. melanogaster

Name

FlyBase ID

FBal0208250

Feature type

allele

Associated gene

Dmel\Rdl

Associated Insertion(s)

Carried in Construct

P{GD4609}

Also Known As

UAS-Rdl-RNAi, UAS-Rdl^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD4609}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD4609

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates amyotrophic lateral sclerosis type 10

modeled by TBPH^Δ23, TBPH^GD6943

(Romano et al., 2021)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal courtship behavior | RU486 conditional, with Scer\GAL4^GS.Ilp2.PU

(Chen et al., 2022)

abnormal sleep, with Scer\GAL4^P2.4.Pdf

(Petruccelli et al., 2015)

abnormal sleep | adult stage, with Scer\GAL4^P2.4.Pdf

(Chaturvedi et al., 2022, Li et al., 2017)

decreased sleep | adult stage, with Scer\GAL4^P2.4.Pdf

(Chaturvedi et al., 2022)

lethal, with Scer\GAL4^Act5C.PI

(Li et al., 2014)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^e22c, Scer\GAL4^sqh.PW

(Tsubouchi et al., 2012)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

The expression of Rdl^GD4609 under the control of Scer\GAL4^P2.4.Pdf leads to a significant decrease in sleep duration during both day time and night time (ZT1-12 and ZT13-24, respectively), and leads to a significant increase in sleep latency period, as compared to controls.

(Li et al., 2017)

Rdl^GD4609 driven by Scer\GAL4^P2.4.Pdf significantly decreases nighttime sleep but does not affect sleep latency in mated females.

(Petruccelli et al., 2015)

Expression of Rdl^GD4609 under the control of Scer\GAL4^Act5C.PI is lethal.

(Li et al., 2014)

Adults expressing Rdl^GD4609 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of Rdl^GD4609 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD4609} insertion line used.

(Neely et al., 2010)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

4609

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Rdl^GD4609, Scer\GAL4^P2.4.Pdf has decreased sleep | adult stage phenotype, non-enhanceable by Gat^33-1

(Chaturvedi et al., 2022)

Rdl^GD4609, Scer\GAL4^P2.4.Pdf has abnormal sleep | adult stage phenotype, non-enhanceable by Gat^33-1

(Chaturvedi et al., 2022)

Suppressed by

Statement

Reference

Rdl^GD4609, Scer\GAL4^GS.Ilp2.PU has abnormal courtship behavior | RU486 conditional phenotype, suppressible | partially by Df(3L)Ilp2-3/+

(Chen et al., 2022)

Rdl^GD4609, Scer\GAL4^P2.4.Pdf has abnormal sleep | adult stage phenotype, suppressible by Fbxl4^Δ45/Fbxl4^Δ45

(Li et al., 2017)

NOT suppressed by

Statement

Reference

Rdl^GD4609, Scer\GAL4^P2.4.Pdf has decreased sleep | adult stage phenotype, non-suppressible by Gat^33-1

(Chaturvedi et al., 2022)

Rdl^GD4609, Scer\GAL4^P2.4.Pdf has abnormal sleep | adult stage phenotype, non-suppressible by Gat^33-1

(Chaturvedi et al., 2022)

NOT Enhancer of

Statement

Reference

Scer\GAL80^ts.αTub84B, Rdl^GD4609, Scer\GAL4^da.PU is a non-enhancer of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

NOT Suppressor of

Statement

Reference

Scer\GAL80^ts.αTub84B, Rdl^GD4609, Scer\GAL4^da.PU is a non-suppressor of lethal - all die during pupal stage | heat sensitive phenotype of Scer\GAL4^da.PU, Scer\GAL80^ts.αTub84B, fzr^RNAi.UAS.WIZ

(Neuert et al., 2017)

Other

Statement

Reference

Dcr-2^UAS.cDa, Rdl^GD4609, Scer\GAL4^elav.PLu has lethal phenotype

(Chung et al., 2009)

Dcr-2^UAS.cDa, Rdl^GD4609, Scer\GAL4^elav.PLu has abnormal circadian rhythm phenotype

(Chung et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The decreased sleep duration and increased sleep latency period observed upon the expression of Rdl^GD4609 under the control of Scer\GAL4^P2.4.Pdf is suppressed by Fbxl4^Δ45 homozygosity.

(Li et al., 2017)

Total nighttime sleep is reduced in para^GEFS+/+ mated females with Rdl^GD4609 driven by Scer\GAL4^P2.4.Pdf (one copy of each), to the same extent as is seen in controls with Rdl^GD4609 driven by Scer\GAL4^P2.4.Pdf; sleep latency is differentially affected, with significant increases in para^GEFS+/+ mated females but no effect in controls.

(Petruccelli et al., 2015)

Flies co-expressing Rdl^GD4609 and Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^elav.PLu show a significant reduction in total sleep time in light:dark (LD) and constant darkness (DD) conditions (flies expressing Dcr-2^Scer\UAS.cDa alone under the control of Scer\GAL4^elav.PLu do not show significant differences in sleep compared to controls).

(Chung et al., 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Rdl^GD4609 is partially rescued by Scer\GAL4^GS.Ilp2.PU/Rdl^UAS.cSa

(Chen et al., 2022)

Comments

Images (0)

Mutant

Wild-type