Scer\GAL4^Dot.PK-mediated expression of sns^GD65 completely abolishes the normal accumulation of ANF-RFP (expressed via Rnor\ANF^{Mhc.T:Disc\RFP}) in pericardial nephrocytes. EM analyses of mutant pericardial nephrocytes reveals reduced numbers of endocytic vesicles, shortened lacuna structures, and abolished nephrocyte diaphragms.

Co-expression of sns^GD65 and Dcr-2^Scer\UAS.cDa under the control of Scer\GAL4^A3.eya results in a male-sterile phenotype, characterised by spermatogenic arrest at the elongated cyst stage and empty seminal vesicles.

Expression under the control of Scer\GAL4^Mef2.PR results in late pupal lethality.

Expression under the control of Scer\GAL4^pnr-MD237 results in bristle morphology defects on the notum in 30-40% of the Scer\GAL4^pnr-MD237 expression domain.

Expression under the control of Scer\GAL4^pnr-MD237 results in the absence of 60-70% of the Scer\GAL4^pnr-MD237-expressing area of the notum.

The numbers of nephrocyte diaphragms in the garland cell nephrocytes of second instar larvae expressing sns^GD65 under the control of Scer\GAL4^sns.GCN is reduced compared to controls, although the NDs that do form appear normal by TEM analysis.

The surface of the garland cell nephrocytes of third instar larvae expressing sns^GD65 under the control of Scer\GAL4^sns.GCN is more uniform than that of control cells when analysed by SEM.

Garland cell nephrocytes from second instar larvae expressing sns^GD65 under the control of Scer\GAL4^sns.GCN show reduced uptake of both dextran 10kD and Alexa Fluor 555 BSA compared to controls after co-incubation with both tracers for 30 seconds.

Garland cell nephrocytes from second instar larvae expressing sns^GD65 under the control of Scer\GAL4^sns.GCN show efficient uptake of dextran 10kD after co-incubation with both Cascade Blue dextran 10kD and fluorescein dextran 500kD for 5 minutes. However, these cells show a very poor uptake of dextran 500kD under these conditions compared to controls.

Dcr-2^UAS.cDa, Scer\GAL4^GMR.PF, Scer\GAL4^ey.PH, sns^GD65 has eye phenotype

Scer\GAL4^pnr-MD237, sns^GD65 has thorax phenotype