FlyBase Allele Report: Dmel\wg[GD5007]

General Information

Symbol

Dmel\wg^GD5007

Species

D. melanogaster

Name

FlyBase ID

FBal0210886

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

Carried in Construct

P{GD5007}

Also Known As

UAS-RNAi-wg, UAS-Wg-RNAi, wg RNAi, UAS-wg^RNAi, Wg^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD5007}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD5007

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Egfr^UAS.cBa, psq^KK111691

(Herranz et al., 2014)

modeled by Csk^Q156Stop, Ras85D^V12.UAS

(Hirabayashi et al., 2013)

modeled by BacA\p35^UAS.cHa, rod^GD8768

(Muzzopappa et al., 2017)

modeled by BacA\p35^UAS.cHa, mud^HMS01458

(Muzzopappa et al., 2017)

modeled by Ras85D^V12.UAS, mud^HMS01458

(Muzzopappa et al., 2017)

ameliorates carcinoma

modeled by scrib^GD11666

(Zhang et al., 2019)

modeled by Ras85D^V12.UAS, l(2)gl⁴

(Zhang et al., 2019)

modeled by egr^UAS.cIa

(Zhang et al., 2019)

modeled by puc^GD1515

(Zhang et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory | RU486 conditional, with Scer\GAL4^{Mef2.247.Switch}

(Tan et al., 2013)

abnormal neurophysiology, with Scer\GAL4^Gli-rL82

(Kerr et al., 2014)

decreased cell number | pupal stage, with Scer\GAL4^GMR.PU

(Cordero and Cagan, 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

cone cell | pupal stage, with Scer\GAL4^GMR.PU

(Cordero and Cagan, 2010)

Detailed Description

Statement

Reference

Neuronal expression of wg^GD5007 under the control of Scer\GAL4^futsch-C380, results in a small but significant decrease in bouton number.

Expression of wg^GD5007 in the subperineurial glia cells, under the control of Scer\GAL4^Gli-rL82, does not affect bouton number, but does significantly increase the mEJP amplitude in third instar larvae.

(Kerr et al., 2014)

Inducible RNAi-mediated knockdown of wg in the adult mushroom bodies, through expression of wg^GD5007 under the control of Scer\GAL4^{Mef2.247.Switch}, leads to impaired 24 hour long-term memory after spaced conditioning, but has no effect on 24 hour memory after massed training or 3 minute memory after 1X conditioning. The results from shock- and odour-avoidance tests in the induced flies are indistinguishable from those obtained in the uninduced control.

(Tan et al., 2013)

42 hours after pupation ommatidia with abnormal numbers of cone cells are seen when wg^GD5007 is expressed under the control of the Scer\GAL4^GMR.PU driver.

(Cordero and Cagan, 2010)

Adults expressing wg^GD5007 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

Expression of wg^GD5007 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) results in viable flies or partial lethality, depending on the particular P{GD5007} insertion line used.

(Neely et al., 2010)

Depending on the insertion line used, expression under the control of Scer\GAL4^Mef2.PR can result in viable flies or undefined lethality.

(Schnorrer et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in loss of bristles on the notum in 0% or 20-30% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

Expression under the control of Scer\GAL4^pnr-MD237 results in a colour difference between the central Scer\GAL4^pnr-MD237 expression domain of the notum and the surrounding lateral region in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

5007

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

wg^GD5007, Scer\GAL4^GMR.PU is a suppressor | partially of visible | adult stage phenotype of Hsap\RPS12^UAS.cKa, Scer\GAL4^GMR.PU

(Katanaev et al., 2020)

wg^GD5007, Scer\GAL4^GMR.PU is a suppressor | partially of decreased size | adult stage phenotype of Hsap\RPS12^UAS.cKa, Scer\GAL4^GMR.PU

(Katanaev et al., 2020)

Scer\GAL4^ptc-559.1/wg^GD5007 is a suppressor | partially of abnormal cell migration | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, egr^UAS.cIa

(Zhang et al., 2019)

Scer\GAL4^ptc-559.1/wg^GD5007 is a suppressor | partially of abnormal cell migration | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, puc^GD1515

(Zhang et al., 2019)

Scer\GAL4^ptc-559.1/wg^GD5007 is a suppressor | partially of abnormal cell migration | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, scrib^GD11666

(Zhang et al., 2019)

wg^GD5007, Scer\GAL4^Act.PU is a suppressor | partially of neoplasia | somatic clone | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, l(2)gl⁴

(Zhang et al., 2019)

wg^GD5007, Scer\GAL4^Act.PU is a suppressor | partially of abnormal cell migration | somatic clone | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, l(2)gl⁴

(Zhang et al., 2019)

wg^GD5007, Scer\GAL4^Act.PU is a suppressor | partially of increased mortality during development | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, l(2)gl⁴

(Zhang et al., 2019)

wg^GD5007, Scer\GAL4^hh.PU is a suppressor of neoplasia | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^hh.PU, mud^HMS01458

(Muzzopappa et al., 2017)

wg^GD5007/Scer\GAL4^hh.PU is a suppressor of neoplasia | larval stage phenotype of BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, rod^GD8768

(Muzzopappa et al., 2017)

wg^GD5007/Scer\GAL4^hh.PU is a suppressor of neoplasia | larval stage phenotype of BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, mud^HMS01458

(Muzzopappa et al., 2017)

wg^GD5007, Scer\GAL4^ap-md544 is a suppressor | partially of neoplasia phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^JF03109

(Herranz et al., 2014)

wg^GD5007, Scer\GAL4^sd-SG29.1 is a suppressor of visible phenotype of Mad^GM8.UAS, Scer\GAL4^sd-SG29.1

(Eivers et al., 2011)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Su(H).GBE/wg^GD5007 is a non-suppressor of neoplasia phenotype of Sox21a⁶

(Zhai et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

wg^GD5007, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Hsap\RPS12^UAS.cKa, Scer\GAL4^GMR.PU

(Katanaev et al., 2020)

Scer\GAL4^ptc-559.1/wg^GD5007 is a suppressor | partially of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, egr^UAS.cIa

(Zhang et al., 2019)

Scer\GAL4^ptc-559.1/wg^GD5007 is a suppressor | partially of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, puc^GD1515

(Zhang et al., 2019)

Scer\GAL4^ptc-559.1/wg^GD5007 is a suppressor | partially of anterior-posterior compartment boundary of the wing disc | third instar larval stage phenotype of Scer\GAL4^ptc-559.1, scrib^GD11666

(Zhang et al., 2019)

wg^GD5007, Scer\GAL4^Act.PU is a suppressor | partially of larval ventral nerve cord | somatic clone | larval stage phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, l(2)gl⁴

(Zhang et al., 2019)

wg^GD5007, Scer\GAL4^Act.PU is a suppressor | partially of eye-antennal disc | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^Act.PU, l(2)gl⁴

(Zhang et al., 2019)

wg^GD5007, Scer\GAL4^hh.PU is a suppressor of wing disc phenotype of Ras85D^V12.UAS, Scer\GAL4^hh.PU, mud^HMS01458

(Muzzopappa et al., 2017)

wg^GD5007/Scer\GAL4^hh.PU is a suppressor of wing disc phenotype of BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, rod^GD8768

(Muzzopappa et al., 2017)

wg^GD5007/Scer\GAL4^hh.PU is a suppressor of wing disc phenotype of BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, mud^HMS01458

(Muzzopappa et al., 2017)

wg^GD5007, Scer\GAL4^sd-SG29.1 is a suppressor of wing margin bristle | ectopic phenotype of Mad^GM8.UAS, Scer\GAL4^sd-SG29.1

(Eivers et al., 2011)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Su(H).GBE/wg^GD5007 is a non-suppressor of adult anterior midgut epithelium phenotype of Sox21a⁶

(Zhai et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of wg^GD5007 in enteroblasts under the control of Scer\GAL4^Su(H).GBE does not reduce tumor formation in the anterior midgut of Sox21a⁶ mutant flies.

(Zhai et al., 2015)

Expression of wg^GD5007 partially suppresses the growth of the massive wing disc tumors seen when Egfr^Scer\UAS.cBa and psq^KK111691 are expressed under the control of Scer\GAL4^ap-md544.

(Herranz et al., 2014)

The ectopic wing margin bristle phenotype caused by expression of Mad^GM8.Scer\UAS under the control of Scer\GAL4^sd-SG29.1 is suppressed by co-expression of wg^GD5007.

(Eivers et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (3)

VDRC

v13351