FlyBase Allele Report: Dmel\loqs[KO]

General Information

Symbol

Dmel\loqs^KO

Species

D. melanogaster

Name

FlyBase ID

FBal0219236

Feature type

allele

Associated gene

Dmel\loqs

Associated Insertion(s)

TI{TI}loqs^KO

Carried in Construct

Key Links

Allele class

amorphic allele - molecular evidence

Mutagen

Nature of the Allele

Allele class

amorphic allele - molecular evidence

(Park et al., 2007)

Mutagen

recombination

(Park et al., 2007)

FLPase

(Park et al., 2007)

I-SceI

(Park et al., 2007)

ends-out gene targeting

(Park et al., 2007)

Progenitor genotype

Associated Insertion(s)

TI{TI}loqs^KO

Cytology

Description

The entire loqs open reading frame has been replaced by an FRT cassette containing the w^+mW.hs marker gene.

(Park et al., 2007)

Allele components

Component

Use(s)

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

female sterile, with loqs^PA.Tag:FLAG

(Fukunaga et al., 2012)

female sterile, with loqs^PA.Tag:FLAG, loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

fertile, with loqs^PB.Tag:FLAG

(Fukunaga et al., 2012)

lethal | embryonic stage | recessive | rescuable maternal effect

(Park et al., 2007)

lethal | pharate adult stage | recessive

(Park et al., 2007)

lethal - all die during embryonic stage

(Fukunaga et al., 2012)

lethal - all die during embryonic stage, with loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

male fertile, with loqs^PA.Tag:FLAG

(Fukunaga et al., 2012)

non-suppressor of variegation

(Fagegaltier et al., 2009)

some die during embryonic stage

(Fukunaga et al., 2012)

some die during embryonic stage, with loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

viable, with loqs^PA.Tag:FLAG

(Fukunaga et al., 2012)

viable, with loqs^PB.Tag:FLAG

(Fukunaga et al., 2012)

Phenotype Manifest In

dorsal appendage, with loqs^PA.Tag:FLAG

(Fukunaga et al., 2012)

dorsal appendage, with loqs^PA.Tag:FLAG, loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

female germline stem cell (with loqs^f00791)

(Park et al., 2007)

female germline stem cell | germline clone

(Park et al., 2007)

germline stem cell, with loqs^PA.Tag:FLAG, loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

Detailed Description

Statement

Reference

loqs^{PA.T:Zzzz\FLAG} restores the embryonic viability of loqs^KO mutants. The rescued males are fertile, however, females lay far fewer eggs than wild-type controls. None of the eggs produced by loqs^KO mutants rescued by loqs^{PA.T:Zzzz\FLAG} hatch, and few of the eggs display normal dorsal appendages.

loqs^KO mutant females rescued by loqs^{PA.T:Zzzz\FLAG} together with loqs^{PD.T:Zzzz\FLAG} lay far fewer eggs than wild-type controls. None of the eggs produced by the rescued females hatch. Few of the eggs laid by the rescued females display normal appendages.

loqs^KO mutant females rescued by either loqs^{PA.T:Zzzz\FLAG} or the combination of loqs^{PA.T:Zzzz\FLAG} and loqs^{PD.T:Zzzz\FLAG} display fewer germline stem cells (GSCs) per germaria than wild-type controls.

The number of germline stem cells (GSCs) in loqs^KO females rescued by all genotypes expressing loqs^{PB.T:Zzzz\FLAG} (loqs^{PA.T:Zzzz\FLAG} together with loqs^{PB.T:Zzzz\FLAG}, or loqs^{PB.T:Zzzz\FLAG} together with loqs^{PD.T:Zzzz\FLAG}, or loqs^{PA.T:Zzzz\FLAG} together with both loqs^{PB.T:Zzzz\FLAG} and loqs^{PD.T:Zzzz\FLAG}) appears normal compared to wild-type.

(Fukunaga et al., 2012)

loqs^KO mutants display a reduction in dsRNA processing and a subsequent ~60% reduction in siRNA levels.

(Marques et al., 2010)

loqs^KO/+ does not significantly suppress the position effect variegation of the Sb¹ allele that is seen in the T(2;3)Sb^V chromosome.

(Fagegaltier et al., 2009)

Homozygous embryos hatch and develop at rates similar to that of wild-type controls and most homozygous larvae undergo pupation. However, viability drops precipitously at the pupa/adult transition; most homozygotes die during eclosion, while approximately 10% emerge but are nearly paralysed and die shortly after.

Homozygous embryos derived from homozygous female germline clones do not develop past the first larval instar stage.

Newly emerged homozygous adult female escapers usually have empty germaria accompanied by one or two terminal stage egg chambers.

loqs^f00791/loqs^KO females have an average of 0.5 germline stem cells (GSCs) per germarium at 10 days after eclosion, compared to an average of 2-3 GSCs per germarium in wild-type females at this age. Differentiated cystoblasts with posterior-positioned fusomes are often seen at the normal location of GSCs in the germaria of loqs^f00791/loqs^KO females.

Homozygous female germline stem cell clones have a half-life of only 6 days, compared to a half-life of 21 days for control clones.

(Park et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

loqs^PA.Tag:FLAG/loqs^KO is a suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs^PB.Tag:FLAG/loqs^KO is a suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs^PB.Tag:FLAG, loqs^PA.Tag:FLAG, loqs^KO is a suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

NOT Suppressor of

Statement

Reference

loqs[+]/loqs^KO is a non-suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs[+], loqs^PA.Tag:FLAG, loqs^KO is a non-suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs[+], loqs^PB.Tag:FLAG, loqs^KO is a non-suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs[+], loqs^PB.Tag:FLAG, loqs^PA.Tag:FLAG, loqs^KO is a non-suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs[+], loqs^PA.Tag:FLAG, loqs^KO, loqs^PD.Tag:FLAG is a non-suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs^PA.Tag:FLAG, loqs^KO, loqs^PD.Tag:FLAG is a non-suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs[+], loqs^PB.Tag:FLAG, loqs^KO, loqs^PD.Tag:FLAG is a non-suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs^PB.Tag:FLAG, loqs^KO, loqs^PD.Tag:FLAG is a non-suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs[+], loqs^PB.Tag:FLAG, loqs^PA.Tag:FLAG, loqs^KO, loqs^PD.Tag:FLAG is a non-suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs^PB.Tag:FLAG, loqs^PA.Tag:FLAG, loqs^KO, loqs^PD.Tag:FLAG is a non-suppressor of visible phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

loqs^PA.Tag:FLAG/loqs^KO is a suppressor of eye phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs^PB.Tag:FLAG/loqs^KO is a suppressor of eye phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs^PB.Tag:FLAG, loqs^PA.Tag:FLAG, loqs^KO is a suppressor of eye phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs^KO is a suppressor of eye phenotype of w^RNAi.GMR

(Marques et al., 2010)

r2d2^S165fsX/loqs^KO is a suppressor of eye phenotype of w^RNAi.GMR

(Marques et al., 2010)

loqs[+]/loqs^KO is a suppressor of ovary phenotype of mei-P26^fs1

(Neumuller et al., 2008)

NOT Suppressor of

Statement

Reference

loqs[+]/loqs^KO is a non-suppressor of eye phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs^PB.Tag:FLAG, loqs^PA.Tag:FLAG, loqs^KO, loqs^PD.Tag:FLAG is a non-suppressor of eye phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs[+], loqs^PA.Tag:FLAG, loqs^KO is a non-suppressor of eye phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs[+], loqs^PB.Tag:FLAG, loqs^KO is a non-suppressor of eye phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs[+], loqs^PB.Tag:FLAG, loqs^PA.Tag:FLAG, loqs^KO is a non-suppressor of eye phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs[+], loqs^PA.Tag:FLAG, loqs^KO, loqs^PD.Tag:FLAG is a non-suppressor of eye phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs^PA.Tag:FLAG, loqs^KO, loqs^PD.Tag:FLAG is a non-suppressor of eye phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs[+], loqs^PB.Tag:FLAG, loqs^KO, loqs^PD.Tag:FLAG is a non-suppressor of eye phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs^PB.Tag:FLAG, loqs^KO, loqs^PD.Tag:FLAG is a non-suppressor of eye phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs[+], loqs^PB.Tag:FLAG, loqs^PA.Tag:FLAG, loqs^KO, loqs^PD.Tag:FLAG is a non-suppressor of eye phenotype of w^RNAi.GMR

(Fukunaga et al., 2012)

loqs^f00791/loqs^KO is a non-suppressor of female germline cell phenotype of bam^unspecified

(Park et al., 2007)

Additional Comments

Genetic Interactions

Statement

Reference

loqs^KO mutant flies carrying two copies of w^IR.GMR exhibit impaired w silencing and display orange-red eyes.

loqs^KO r2d2^S165fsX double mutant flies carrying two copies of w^IR.GMR exhibit impaired w silencing and display orange-red eyes.

loqs^KO r2d2^S165fsX double mutants display a reduction in dsRNA processing and a subsequent ~80% reduction in siRNA levels.

(Marques et al., 2010)

The germ cell tumour phenotype seen in bam^unspecified females is not suppressed by loqs^f00791/loqs^KO. The double mutant germaria retain germline stem cells.

(Park et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

loqs^KO is rescued by loqs^PB.Tag:FLAG

(Fukunaga et al., 2012)

loqs^f00791/loqs^KO is rescued by loqs^PB.Tag:MYC

(Park et al., 2007)

loqs^f00791/loqs^KO is rescued by Scer\GAL4^{VP16.nanos.UTR}/loqs^{PB.UASp.Tag:MYC}

(Park et al., 2007)

loqs^KO is rescued by loqs^PB.Tag:MYC

(Park et al., 2007)

Partially rescued by

loqs^KO is partially rescued by loqs^PA.Tag:FLAG

(Fukunaga et al., 2012)

loqs^KO is partially rescued by loqs^PA.Tag:FLAG/loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

Not rescued by

loqs^KO is not rescued by loqs^PD.Tag:FLAG

(Fukunaga et al., 2012)

loqs^f00791/loqs^KO is not rescued by loqs^PA.Tag:MYC

(Park et al., 2007)

loqs^f00791/loqs^KO is not rescued by loqs^{PB.UASp.Tag:MYC}/Scer\GAL4^bab1-Agal4-5

(Park et al., 2007)

loqs^f00791/loqs^KO is not rescued by Scer\GAL4^C587/loqs^{PB.UASp.Tag:MYC}

(Park et al., 2007)

Comments

loqs^{PB.T:Zzzz\FLAG} restores the embryonic viability of loqs^KO mutants. The rescued flies are fertile and females lay normal eggs. The number of germline stem cells (GSCs) in loqs^KO females is also restored to wild-type levels by loqs^{PB.T:Zzzz\FLAG}.

loqs^{PD.T:Zzzz\FLAG} fails to restore the embryonic viability of loqs^KO mutants.

(Fukunaga et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

65411

w^*; TI{TI}loqs^KO P{neoFRT}40A/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

loqs-KO

(Okamura et al., 2008)

loqs^KO

(Zhang et al., 2023, de Faria et al., 2022, Porrazzo et al., 2022, Eyk et al., 2019, Fukunaga et al., 2012, Han et al., 2011, Okamura et al., 2011, Marques et al., 2010, Fagegaltier et al., 2009, Martin et al., 2009, Zhou et al., 2009, Neumuller et al., 2008, Park et al., 2007, Park et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

Open Close