FlyBase Allele Report: Dmel\vkg[NIG.16858R]

General Information

Symbol

Dmel\vkg^NIG.16858R

Species

D. melanogaster

Name

FlyBase ID

FBal0220376

Feature type

allele

Associated gene

Dmel\vkg

Associated Insertion(s)

Carried in Construct

P{NIG.16858R}

Also Known As

UAS-vkg.RNAi^NIG.16858R-3

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Japanese National Institute of Genetics, 2012.5.21)

Mutagen

in vitro construct

(Japanese National Institute of Genetics, 2012.5.21, Junion et al., 2007)

Progenitor genotype

Carried in construct

P{NIG.16858R}

Cytology

Description

UASt regulatory sequences drive expression of a 500bp inverted repeat.

(Japanese National Institute of Genetics, 2012.5.21)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-16858R

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

A1-7 segment border muscle cell, with Scer\GAL4^how-24B

(Junion et al., 2007)

Detailed Description

Statement

Reference

Expressing vkg^NIG.16858R under the control of Scer\GAL4^bs.Term does not cause air-filling defects or any obvious trachea branching defect in the trachea terminal cells region.

(Ríos-Barrera et al., 2017)

Embryos expressing vkg^NIG.16858R under the control of Scer\GAL4^how-24B show abnormal attachment of the segment border muscle (SBM) to adjacent muscles and an abnormal SBM shape.

(Junion et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Col4a1^GD12784/vkg^NIG.16858R, Scer\GAL4^r4 is a suppressor of melanotic mass phenotype | larval stage phenotype of Scer\GAL4^r4, shi^NIG.18102R

(Zang et al., 2015)

Col4a1^GD12784/vkg^NIG.16858R, Scer\GAL4^r4 is a suppressor of melanotic mass phenotype | larval stage phenotype of Scer\GAL4^r4, Tl^10b.UAS

(Zang et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Col4a1^GD12784/vkg^NIG.16858R, Scer\GAL4^r4 is a suppressor of embryonic/larval fat body | larval stage phenotype of Scer\GAL4^r4, shi^NIG.18102R

(Zang et al., 2015)

Col4a1^GD12784/vkg^NIG.16858R, Scer\GAL4^r4 is a suppressor of melanotic mass | larval stage phenotype of Scer\GAL4^r4, Tl^10b.UAS

(Zang et al., 2015)

NOT Suppressor of

Statement

Reference

Scer\GAL4^apolpp.PB/vkg^NIG.16858R is a non-suppressor of fat body phenotype of Scer\GAL4^apolpp.PB, Tango1^NIG.11098R

(Ríos-Barrera et al., 2017)

Additional Comments

Genetic Interactions

Statement

Reference

Simultaneous co-expression of both vkg^NIG.16858R and Cg25C^GD12784 suppresses the melanotic fat body phenotype caused by expression of shi^NIG.18102R under the control of Scer\GAL4^r4.

Simultaneous co-expression of both vkg^NIG.16858R and Cg25C^GD12784 suppresses the melanotic phenotype caused by expression of Tl^10b.Scer\UAS under the control of Scer\GAL4^r4.

(Zang et al., 2015)

At a permissive temperature (18[o]C) coexpression of vkg^G454 and vkg^NIG.16858R with Cg25C^GD12784 under the control of Scer\GAL4^Cg.PA and Scer\GAL80^ts.αTub84B knocks down expression of vkg. These flies develop into normal adults, although pupal lethality results when the larvae are transferred to restrictive temperature after completion of embryogenesis. Dissection of these larvae 96 hours after the temperature shift reveals highly aberrant shapes in several organs. Imaginal discs, consisting of highly columnar epithelial cells, appear flattened while the ventral nerve cord portion of the central nervous system is strikingly elongated, reminiscent of embryonic defects in ventral nerve cord condensation. Additionally, the ducts and imaginal rings of the salivary glands are dilated, resulting in an expanded lumen.

(Pastor-Pareja and Xu, 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

NIG-Fly

16858R-1

P{NIG.16858R}1

16858R-3

P{NIG.16858R}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

vkg^NIG.16858R

vkgⁱ

(Sun et al., 2021)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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