FlyBase Allele Report: Dmel\Manf[Δ96]

General Information

Symbol

Dmel\Manf^Δ96

Species

D. melanogaster

Name

FlyBase ID

FBal0221493

Feature type

allele

Associated gene

Dmel\Manf

Associated Insertion(s)

Carried in Construct

Also Known As

DmMANF^Δ96

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

P{SUPor-P}CG14879^KG03250

(Palgi et al., 2009)

Cytology

Description

Imprecise excision of the P{SUPor-P}CG14879^KG03250 insertion, resulting in a deletion that removes 278bp of the Manf open reading frame, including the first 2 exons and part of the third exon.

(Palgi et al., 2009)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of neurodegenerative disease

CEA

(Palgi et al., 2009)

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/first instar larval cuticle | maternal effect

(Palgi et al., 2009)

embryonic/larval cell body glial cell | embryonic stage 17 | maternal effect

(Palgi et al., 2009)

eye | adult stage

(Neves et al., 2016)

larval ventral nerve cord | embryonic stage 17 | maternal effect

(Palgi et al., 2009)

neurite & dopaminergic neuron | larval stage

(Palgi et al., 2009)

neuropil | embryonic stage 17 | maternal effect

(Palgi et al., 2009)

Detailed Description

Statement

Reference

Manf^Δ96/+ heterozygous adult flies show decreased eye tissue regeneration capacity upon UV radiation induced damage (inflicted during pupal stage) compared to wild-type.

(Neves et al., 2016)

Manf^Δ96 homozygous clones are able to survive and differentiate into glia and dopaminergic neurons, similar to surrounding control cells.

(Stratoulias and Heino, 2015)

Mutant larvae show a significant increase in dopamine levels in total larval lysates compared to wild type.

(Lindström et al., 2013)

Homozygotes die as later first instar larvae or immediately after the delayed first moult with defects in behaviour; the larvae atypically wander away from the food, then move more slowly and finally freeze, immobilised but still responding to touch.

Embryonic neural development occurs normally in homozygotes.

The volume of DA neurites is significantly diminished in homozygous larvae, whereas the neuronal somae persist. The neuritic degeneration occurs mostly in the abdominal area of the ventral nerve cord. There is no significant change in the neurite volume of serotonergic neurons or in the volume of the motorneuron subpopulation in these larvae.

Manf^Δ96 embryos lacking both maternal and zygotic Manf[+] function develop normally until late stage 16, but show severe defects thereafter; the ventral nerve cord (VNC) becomes overcondensed compared to wild type. At early stage 16, the neuropile of the VNC still resembles that of wild type, but by late stage 17, degradation of axonal membranes is seen, starting at the border of the glia and neuropile. In addition, cell body glial cells appear highly electron dense and contain remnants of cell debris. In the VNC, dying cells with poor and bleached cytoplasm, swollen nuclei and with dilated endoplasmic reticulum and other organelles are seen, which is atypical for conventional apoptosis. The cuticle of these embryos is defective; all cuticular layers are disorganised.

(Palgi et al., 2009)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, suppressible by Scer\GAL4^da.G32/Hsap\MANF^UAS.WT

(Lindström et al., 2013)

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, suppressible by Hsap\CDNF^UAS.WT/Scer\GAL4^da.G32

(Lindström et al., 2013)

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, suppressible by Scer\GAL4^69B/Hsap\MANF^UAS.WT

(Lindström et al., 2013)

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, suppressible by Scer\GAL4^da.G32/Hsap\MANF::Hsap\CDNF^UAS.cLa

(Lindström et al., 2013)

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, suppressible by Scer\GAL4^da.G32/Hsap\MANF^ΔRTDL.UAS

(Lindström et al., 2013)

Manf^Δ96 has lethal | recessive | larval stage phenotype, suppressible by Hsap\MANF^UAS.9C/Scer\GAL4^Tub.PU

(Palgi et al., 2009)

Manf^Δ96 has lethal | recessive | larval stage phenotype, suppressible by Hsap\MANF^UAS.9C/Scer\GAL4^da.G32

(Palgi et al., 2009)

NOT suppressed by

Statement

Reference

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, non-suppressible by Scer\GAL4^da.G32/Hsap\MANF^N.UAS

(Lindström et al., 2013)

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, non-suppressible by Hsap\MANF^C.UAS/Scer\GAL4^da.G32

(Lindström et al., 2013)

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, non-suppressible by Scer\GAL4^da.G32/Hsap\MANF^C.Δss.UAS

(Lindström et al., 2013)

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, non-suppressible by Scer\GAL4^da.G32/Hsap\CDNF^N.UAS

(Lindström et al., 2013)

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, non-suppressible by Hsap\CDNF^C.UAS/Scer\GAL4^da.G32

(Lindström et al., 2013)

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, non-suppressible by Hsap\CDNF^C.Δss.UAS/Scer\GAL4^da.G32

(Lindström et al., 2013)

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, non-suppressible by Hsap\CDNF^UAS.WT/Scer\GAL4^69B

(Lindström et al., 2013)

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, non-suppressible by Scer\GAL4^69B/Hsap\MANF::Hsap\CDNF^UAS.cLa

(Lindström et al., 2013)

Manf^Δ96 has lethal - all die during first instar larval stage phenotype, non-suppressible by Hsap\MANF^ΔRTDL.UAS/Scer\GAL4^69B

(Lindström et al., 2013)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Scer\GAL4^da.G32-mediated expression of Hsap\MANF^Scer\UAS.WT or Hsap\CDNF^Scer\UAS.WT efficiently rescues Manf^Δ96 mutant lethality to adulthood.

Scer\GAL4^da.G32-mediated expression of Hsap\MANF^N.Scer\UAS, Hsap\MANF^C.Scer\UAS or Hsap\MANF^{C.Δss.Scer\UAS} does not rescue Manf^Δ96 larval lethality.

Scer\GAL4^da.G32-mediated expression of Hsap\CDNF^N.Scer\UAS, Hsap\CDNF^C.Scer\UAS or Hsap\CDNF^{C.Δss.Scer\UAS} does not rescue Manf^Δ96 larval lethality.

Scer\GAL4^69B-mediated expression of Hsap\MANF^Scer\UAS.WT but not Hsap\CDNF^Scer\UAS.WT rescues Manf^Δ96 mutant lethality to adulthood.

Scer\GAL4^da.G32-mediated, but not Scer\GAL4^69B-mediated, expression of Hsap\MANF::Hsap\CDNF^Scer\UAS rescues Manf^Δ96 mutant lethality to adulthood.

Scer\GAL4^da.G32-mediated, but not Scer\GAL4^69B-mediated, expression of Hsap\MANF^{ΔRTDL.Scer\UAS} rescues Manf^Δ96 mutant lethality to adulthood.

(Lindström et al., 2013)

Expression of Hsap\MANF^Scer\UAS.cPa under the control of one of Scer\GAL4^da.G32 or Scer\GAL4^tub significantly rescues the larval lethality of Manf^Δ96 animals (assayed by survival into the pupal stage).

(Palgi et al., 2009)

Complementation and Rescue Data

Rescued by

Manf[+]/Manf^Δ96 is rescued by Scer\GAL4^Hml.Δ/Manf^UAS.cPa

(Neves et al., 2016)

Manf^Δ96 is rescued by Scer\GAL4^da.G32/Manf^UAS.cPa

(Lindström et al., 2013)

Manf^Δ96 is rescued by Manf^UAS.cPa/Scer\GAL4^69B

(Lindström et al., 2013)

Manf^Δ96 is rescued by Scer\GAL4^da.G32/Manf^ΔRSEL.UAS

(Lindström et al., 2013)

Manf^Δ96 is rescued by Manf^NMG1.UAS/Scer\GAL4^da.G32

(Lindström et al., 2013)

Manf^Δ96 is rescued by Manf^NMG2.UAS/Scer\GAL4^da.G32

(Lindström et al., 2013)

Manf^Δ96 is rescued by Manf^NMG2.UAS/Scer\GAL4^69B

(Lindström et al., 2013)

Manf^Δ96 is rescued by Scer\GAL4^da.G32/Manf^UAS.cPa

(Palgi et al., 2009)

Manf^Δ96 is rescued by Scer\GAL4^Tub.PU/Manf^UAS.cPa

(Palgi et al., 2009)

Manf^Δ96 is rescued by Manf^UAS.cPa/Scer\GAL4^69B

(Palgi et al., 2009)

Partially rescued by

Manf^Δ96 is partially rescued by Manf^ΔRSEL.UAS/Scer\GAL4^69B

(Lindström et al., 2013)

Not rescued by

Manf^Δ96 is not rescued by Scer\GAL4^da.G32/Manf^N.UAS

(Lindström et al., 2013)

Manf^Δ96 is not rescued by Manf^C.UAS/Scer\GAL4^da.G32/Manf^N.UAS

(Lindström et al., 2013)

Manf^Δ96 is not rescued by Scer\GAL4^da.G32/Manf^N.UAS/Manf^C.Δss.UAS

(Lindström et al., 2013)

Manf^Δ96 is not rescued by Scer\GAL4^da.G32/Manf^N.Δss.UAS

(Lindström et al., 2013)

Manf^Δ96 is not rescued by Manf^C.UAS/Scer\GAL4^da.G32/Manf^N.Δss.UAS

(Lindström et al., 2013)

Manf^Δ96 is not rescued by Scer\GAL4^da.G32/Manf^N.Δss.UAS/Manf^C.Δss.UAS

(Lindström et al., 2013)

Manf^Δ96 is not rescued by Manf^C.UAS/Scer\GAL4^da.G32

(Lindström et al., 2013)

Manf^Δ96 is not rescued by Scer\GAL4^da.G32/Manf^C.Δss.UAS

(Lindström et al., 2013)

Manf^Δ96 is not rescued by Manf^Δss.UAS/Scer\GAL4^da.G32

(Lindström et al., 2013)

Manf^Δ96 is not rescued by Scer\GAL4^da.G32/Manf^C129S.UAS

(Lindström et al., 2013)

Manf^Δ96 is not rescued by Manf^NMG1.UAS/Scer\GAL4^69B

(Lindström et al., 2013)

Manf^Δ96 is not rescued by Manf^UAS.cPa/Scer\GAL4^path-c135

(Palgi et al., 2009)

Comments

The increased severity of eye degeneration after exposure of one eye to UV irradiation during pupal stage characteristic for Manf^Δ96/+ adults can be rescued by expression of Manf^Scer\UAS.cPa under the control of Scer\GAL4^Hml.Δ.

(Neves et al., 2016)

Manf^Scer\UAS.cPa expression driven by Scer\GAL4^da.G32 rescues the early larval lethality of Manf^Δ96 mutants and normalizes Manf^Δ96 larval dopamine to wild type levels.

Expression of Manf^N.Scer\UAS, Manf^{N.Δss.Scer\UAS}, Manf^C.Scer\UAS, or Manf^{C.Δss.Scer\UAS} driven by Scer\GAL4^da.G32 does not rescue the early larval lethality of Manf^Δ96 mutants.

Manf^N.Scer\UAS and Manf^C.Scer\UAS co-expression driven by Scer\GAL4^da.G32 does not rescue the early larval lethality of Manf^Δ96 mutants.

Manf^N.Scer\UAS and Manf^{C.Δss.Scer\UAS} co-expression driven by Scer\GAL4^da.G32 does not rescue the early larval lethality of Manf^Δ96 mutants.

Manf^{N.Δss.Scer\UAS} and Manf^C.Scer\UAS co-expression driven by Scer\GAL4^da.G32 does not rescue the early larval lethality of Manf^Δ96 mutants.

Manf^{N.Δss.Scer\UAS} and Manf^{C.Δss.Scer\UAS} co-expression driven by Scer\GAL4^da.G32 does not rescue the early larval lethality of Manf^Δ96 mutants.

Manf^{Δss.Scer\UAS} expression driven by Scer\GAL4^da.G32 does not rescue the early larval lethality of Manf^Δ96 mutants. Increasing the copy number of the driver and/or responder transgene makes no difference.

Manf^{ΔRSEL.Scer\UAS} expression driven by Scer\GAL4^da.G32 rescues the early larval lethality of Manf^Δ96 mutants to adulthood, and normalizes Manf^Δ96 larval dopamine to wild type levels.

Manf^{C129S.Scer\UAS} expression driven by Scer\GAL4^da.G32 does not rescue the early larval lethality of Manf^Δ96 mutants, though one of three insertions did result in very few rescued pupae.

Manf^{NMG1.Scer\UAS} expression driven by Scer\GAL4^da.G32 rescues the early larval lethality of Manf^Δ96 mutants, and normalizes Manf^Δ96 larval dopamine to wild type levels.

Manf^{NMG2.Scer\UAS} expression driven by Scer\GAL4^da.G32 rescues the early larval lethality of Manf^Δ96 mutants, and normalizes Manf^Δ96 larval dopamine to wild type levels.

Manf^Scer\UAS.cPa expression driven by Scer\GAL4^69B rescues the early larval lethality of Manf^Δ96 mutants.

Manf^{NMG2.Scer\UAS} expression driven by Scer\GAL4^69B rescues the early larval lethality of Manf^Δ96 mutants.

Manf^{ΔRSEL.Scer\UAS} expression driven by Scer\GAL4^69B only partially rescues the early larval lethality of Manf^Δ96 mutants.

Manf^{NMG1.Scer\UAS} expression driven by Scer\GAL4^69B does not rescue the early larval lethality of Manf^Δ96 mutants.

(Lindström et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

DmMANF^Δ96

(Stratoulias and Heino, 2015, Palgi et al., 2009)

DmManf^Δ96

(Lindström et al., 2017, Lindström et al., 2013)

Manf^Δ96

manf^mut96

(Neves et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (5)

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