FlyBase Allele Report: Dmel\piwi[KK105350]

General Information

Symbol

Dmel\piwi^KK105350

Species

D. melanogaster

Name

FlyBase ID

FBal0233505

Feature type

allele

Associated gene

Dmel\piwi

Associated Insertion(s)

Carried in Construct

P{KK105350}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK105350}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK105350

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell number | oogenesis, with Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

abnormal cell shape | oogenesis, with Scer\GAL4^C587

(Ma et al., 2014)

decreased cell number | oogenesis, with Scer\GAL4^C587

(Ma et al., 2014)

female sterile, with Scer\GAL4^nanos.PG

(Czech et al., 2013)

increased cell death | oogenesis, with Scer\GAL4^C587

(Ma et al., 2014)

increased cell number | oogenesis, with Scer\GAL4^C587

(Ma et al., 2014)

Phenotype Manifest In

escort cell, with Scer\GAL4^C587

(Ma et al., 2014)

female germline stem cell, with Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

female germline stem cell, with Scer\GAL4^C587

(Ma et al., 2014)

germarium, with Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

germarium, with Scer\GAL4^C587

(Ma et al., 2014)

germarium cap cell, with Scer\GAL4^bab1-Gal4

(Lin et al., 2020)

Detailed Description

Statement

Reference

The expression of piwi^KK105350 under the control of Scer\GAL4^C587 at room temperature conditions leads to germarium defects: germ line stem cells range from completely absent to increased in number, some locating away from cap cells; escort cells are severely decreased in number, show increases in DNA damage and in apoptosis, and lose their long cellular processes, as compared to controls; similar germ line cell defects are observed in adults kept at 18[o]C throughout development and shifted to 29[o]C upon eclosion, but not in adults kept continuously at 18[o]C throughout development and adulthood.

(Ma et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Scer\GAL4^bab1-Gal4, piwi^KK105350 has abnormal cell number | oogenesis phenotype, non-enhanceable by p53^-ns

(Lin et al., 2020)

Scer\GAL4^bab1-Gal4, piwi^KK105350 has abnormal cell number | oogenesis phenotype, non-enhanceable by Toll-7^HM05230/Scer\GAL4^bab1-Gal4