FlyBase Allele Report: Dmel\dpp[JF01371]

General Information

Symbol

Dmel\dpp^JF01371

Species

D. melanogaster

Name

FlyBase ID

FBal0219809

Feature type

allele

Associated gene

Dmel\dpp

Associated Insertion(s)

Carried in Construct

P{TRiP.JF01371}

Also Known As

dpp^RNAi, dpp RNAi, UAS-Dpp-Rnai, UAS-dpp^RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.1.15)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Perkins et al., 2009.1.15)

Mutagen

in vitro construct

(Perkins et al., 2009.1.15)

Progenitor genotype

Carried in construct

P{TRiP.JF01371}

Cytology

Description

UAS regulatory sequences drive expression of an inverted repeat.

(Perkins et al., 2009.1.15)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-JF01371

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by Egfr^UAS.cBa, psq^KK111691

(Herranz et al., 2014)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut progenitor cell, with Scer\GAL4^GSG5966

(Mathur et al., 2010)

cystoblast | progressive, with Scer\GAL4^C587

(Liu et al., 2015)

cystoblast | progressive, with Scer\GAL4^GMR25A11

(Liu et al., 2015)

female germline stem cell | progressive, with Scer\GAL4^C587

(Liu et al., 2015)

female germline stem cell | progressive, with Scer\GAL4^GMR25A11

(Liu et al., 2015)

germarium, with Scer\GAL4^C587

(Liu et al., 2015)

germarium, with Scer\GAL4^GMR25A11

(Liu et al., 2015)

tarsal segment, with Scer\GAL4^Dll.PU

(Zhe et al., 2020)

tarsal segment, with Scer\GAL4^ptc.PU

(Zhe et al., 2020)

Detailed Description

Statement

Reference

Expression of dpp^JF01371 under the control of Scer\GAL4^Mhc.PW leads to an increase in the spontaneous locomotion particularly in proximity to the food and an increase in the probability of proboscis extension reflex (also occurs with Scer\GAL4^GS.Act88F and RU486 feeding) when compared to control adults.

(Robles-Murguia et al., 2020)

Ovaries in flies expressing dpp^JF01371 under the control of Scer\GAL4^C587 have germaria with fewer spectrosome-containing cells than control germaria; germaria exhibit significant loss of cystoblast bodies and germline stem cells over time; and the number of dividing cystoblasts (but not the number of dividing germline stem cells) is higher, as compared to controls.

Germaria in flies expressing dpp^JF01371 under the control of Scer\GAL4^GMR25A11 exhibit significant loss of cystoblast bodies and germline stem cells over time, as compared to controls.

(Liu et al., 2015)

The expression of dpp^JF01371 under the control of Scer\GAL4^C587 does not lead to any obvious defects in the number of germ line stem cells in the germarium, as compared to controls.

(Ma et al., 2014)

Scer\GAL4^Switch1.PC-driven expression of dpp^JF01371 results in precocious adult midgut progenitor cell differentiation into polyploid enterocyte-like cells which start to break away from wild-type adult midgut progenitor cells.

(Mathur et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^C587/dpp^JF01371 is a suppressor | partially of increased cell number phenotype of Scer\GAL4^C587, ci^GD1403

(Liu et al., 2015)

dpp^JF01371, Scer\GAL4^ap-md544 is a suppressor | partially of neoplasia phenotype of Egfr^UAS.cBa, Scer\GAL4^ap-md544, psq^JF03109

(Herranz et al., 2014)

Scer\GAL4^C587/dpp^JF01371 is a suppressor | partially of increased cell number | oogenesis phenotype of Scer\GAL4^C587, piwi^KK105350

(Ma et al., 2014)

Scer\GAL4^C587/dpp^JF01371 is a suppressor | partially of increased cell number | oogenesis phenotype of Scer\GAL4^C587, piwi^HMS00606

(Ma et al., 2014)

Other

Statement

Reference

Scer\GAL4^C587, bam^hs.PO, dpp^JF01371 has decreased cell number | heat sensitive phenotype

(Liu et al., 2015)

Scer\GAL4^C587, bam^Δ86, bam^hs.PO, dpp^JF01371 has decreased cell number | heat sensitive phenotype

(Liu et al., 2015)

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^C587/dpp^JF01371 is a suppressor | partially of germarium phenotype of Scer\GAL4^C587, ci^GD1403

(Liu et al., 2015)

Scer\GAL4^C587/dpp^JF01371 is a suppressor | partially of germarium phenotype of Scer\GAL4^C587, piwi^KK105350

(Ma et al., 2014)

Scer\GAL4^C587/dpp^JF01371 is a suppressor | partially of female germline stem cell phenotype of Scer\GAL4^C587, piwi^KK105350

(Ma et al., 2014)

Scer\GAL4^C587/dpp^JF01371 is a suppressor | partially of germarium phenotype of Scer\GAL4^C587, piwi^HMS00606

(Ma et al., 2014)

Scer\GAL4^C587/dpp^JF01371 is a suppressor | partially of female germline stem cell phenotype of Scer\GAL4^C587, piwi^HMS00606

(Ma et al., 2014)

Other

Statement

Reference

Scer\GAL4^C587, bam^hs.PO, dpp^JF01371 has germarium | heat sensitive phenotype

(Liu et al., 2015)

Scer\GAL4^C587, bam^hs.PO, dpp^JF01371 has female germline stem cell | heat sensitive phenotype

(Liu et al., 2015)

Scer\GAL4^C587, bam^Δ86, bam^hs.PO, dpp^JF01371 has germarium | heat sensitive phenotype

(Liu et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of dpp^JF01371 under the control of Scer\GAL4^C587 in flies expressing bam^hs.PO induced via heat shock results in germaria exhibiting complete loss of germline stem cells 1 day after heat shock, similar to those expressing bam^hs.PO alone; however, at 7 days after heat shock, the proportion of germaria that show restoration of germline stem cells is substantially lower than that of flies expressing bam^hs.PO alone.

Expression of dpp^JF01371 under the control of Scer\GAL4^C587 in flies expressing bam^hs.PO induced via heat shock in a bam^Δ86/bam^Δ86 background suppresses the recovery of spectrosome-containing cells at 48 h after heat shock seen in flies expressing bam^hs.PO alone in a bam^Δ86/bam^Δ86 background.

Co-expression of dpp^JF01371 partially suppresses the formation of supernumerary spectrosome-containing cells in germaria of flies expressing ci^GD1403 under the control of Scer\GAL4^C587.

(Liu et al., 2015)

Expression of dpp^JF01371 partially suppresses the growth of the massive wing disc tumors seen when Egfr^Scer\UAS.cBa and psq^KK111691 are expressed under the control of Scer\GAL4^ap-md544.

(Herranz et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

25782

y¹ v¹; P{TRiP.JF01371}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

dpp^BL25782

(Liu et al., 2015)

dpp^JF01371

dpp^RNAi

(Su et al., 2018)

dpp^dsRNA

(Luo et al., 2015)

Name Synonyms

Secondary FlyBase IDs

References (23)

Report Sections

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