FlyBase Allele Report: Scer\GAL4[GSG5966]

General Information

Symbol

Scer\GAL4^GSG5966

Species

S. cerevisiae

Name

FlyBase ID

FBal0280522

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

P{Switch2}GSG5966

Carried in Construct

Also Known As

5966GS-GAL4, 5966::GS, 5966GS, 5966-GS, 5966-GS-Gal4, pSwitch^PC, 5966-GeneSwitch

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Associated Insertion(s)

P{Switch2}GSG5966

Cytology

Description

Allele components

Component

Use(s)

Inserted element

P{Switch2}

enhancer trap

Encoded product / tool

GAL4::GS

binary expression system - small molecule-regulated driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

second instar larval stage -- third instar larval stage

peripheral cell

(Mathur et al., 2010)

third instar larval stage

organism | ubiquitous

(Nicholson et al., 2008)

adult stage

enterocyte

(Guo et al., 2014)

enteroblast

(Guo et al., 2014)

Additional Information

Statement

Reference

Drives mifepristone-inducible expression in peripheral cells, enteroblasts, and enterocytes.

(FlyBase Curators, 2017)

Pswitch[PC]

(Mathur et al., 2010)

ScerGAL4^GSG5966 drives mifepristone-inducible expression throughout the larva. There is weak uninduced expression in oenocytes, the gut, and the salivary glands.

(Nicholson et al., 2008)

Marker for

peripheral cell

(Mathur et al., 2010)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult midgut, with AMPKα^UAS.cMa

(Lee et al., 2018)

adult midgut, with gig^UAS.cUa

(Lee et al., 2018)

adult midgut, with Traf-like^UAS.Tag:V5

(Lee et al., 2018)

adult midgut, with wts^UAS.cUa

(Lee et al., 2018)

adult midgut progenitor cell, with dpp^JF01371

(Mathur et al., 2010)

adult midgut progenitor cell | late third instar larval stage, with BacA\p35^UAS.cHa

(Mathur et al., 2010)

adult midgut progenitor cell | late third instar larval stage, with rpr^UAS.C

(Mathur et al., 2010)

adult posterior midgut epithelium, with Nrg^HMS01638

(Resnik-Docampo et al., 2021)

autophagosome | adult stage | RU486 conditional, with gig^UAS.cUa

(Lee et al., 2018)

autophagosome | adult stage | RU486 conditional, with Traf-like^UAS.Tag:V5

(Lee et al., 2018)

embryonic/larval midgut | late third instar larval stage, with BacA\p35^UAS.cHa

(Mathur et al., 2010)

embryonic/larval midgut | third instar larval stage, with rpr^UAS.C

(Mathur et al., 2010)

intestinal stem cell | RU486 conditional, with PGRP-SC2^UAS.cGa

(Guo et al., 2014)

lipid droplet | adult stage | RU486 conditional, with AMPKα^UAS.cMa

(Lee et al., 2018)

lipid droplet | adult stage | RU486 conditional, with gig^UAS.cUa

(Lee et al., 2018)

lipid droplet | adult stage | RU486 conditional, with Traf-like^UAS.Tag:V5

(Lee et al., 2018)

lipid droplet | adult stage | RU486 conditional, with wts^UAS.cUa

(Lee et al., 2018)

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966, Diap1^UAS.cHa is a suppressor | RU486 conditional of increased cell number | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966, Diap1^UAS.cHa is a suppressor | RU486 conditional of decreased cell number | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, puc^UAS.cMa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of increased cell number | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, puc^UAS.cMa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of decreased cell number | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

bsk^DN.UAS, Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of increased cell number | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

bsk^DN.UAS, Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of decreased cell number | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, BacA\p35^UAS.cUa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of increased cell number | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, BacA\p35^UAS.cUa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of decreased cell number | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Phenotype Manifest In

Suppressor of

Statement

Reference

Uev1A^KK110298/Scer\GAL4^GSG5966 is a suppressor of endosome | adult stage phenotype of Cad99C^UAS.GFP, Scer\GAL4^NP0001

(Lee et al., 2018)

Uev1A^KK110298/Scer\GAL4^GSG5966 is a suppressor of adult enterocyte phenotype of Cad99C^UAS.GFP, Scer\GAL4^NP0001

(Lee et al., 2018)

Scer\GAL4^bun-GSG5961, BacA\p35^UAS.cUa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional | cell non-autonomous of intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966, Diap1^UAS.cHa is a suppressor | RU486 conditional of intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966, Diap1^UAS.cHa is a suppressor | RU486 conditional of intestinal stem cell of posterior adult midgut epithelium | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, puc^UAS.cMa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Scer\GAL4^bun-GSG5961, puc^UAS.cMa, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of intestinal stem cell of posterior adult midgut epithelium | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

bsk^DN.UAS, Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

bsk^DN.UAS, Scer\GAL4^bun-GSG5961, Scer\GAL4^GSG5966 is a suppressor | RU486 conditional of intestinal stem cell of posterior adult midgut epithelium | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

PswitchPC

(Mathur et al., 2010)

Scer\GAL4^GSG5966

Scer\GAL4^Switch1.PC

Name Synonyms

Secondary FlyBase IDs

FBal0243219
FBal0269657

References (27)

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