FlyBase Allele Report: Dmel\Rab3[KK108633]

General Information

Symbol

Dmel\Rab3^KK108633

Species

D. melanogaster

Name

FlyBase ID

FBal0235703

Feature type

allele

Associated gene

Dmel\Rab3

Associated Insertion(s)

Carried in Construct

P{KK108633}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Keleman et al., 2009.8.5)

Mutagen

in vitro construct

(Keleman et al., 2009.8.5)

Progenitor genotype

Carried in construct

P{KK108633}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Keleman et al., 2009.8.5)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-KK108633

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

presynaptic active zone | larval stage, with Scer\GAL4^elav.PU

(Kang et al., 2024)

type I bouton | larval stage, with Scer\GAL4^elav.PU

(Kang et al., 2024)

Detailed Description

Statement

Reference

Expression of Rab3^KK108633 (together with UAS-Dicer2 to enhance RNAi efficiency) under the control of either Scer\GAL4^phm.PU or Scer\GAL4^2-286 is second/third larval stage lethal.

(Nakaoka et al., 2017)

Expression of Rab3^KK108633 (along with UAS-Dcr-2 expression) driven by Scer\GAL4^phm.PO leads to developmental delay/arrest at the larval stage (around 5% at L1/2 and 5% at L3). Arrested third instar larvae also show an overgrowth phenotype due to the extended larval feeding period. Those larvae that do initiate pupariation are significantly delayed in doing so and exhibit overgrowth phenotypes. Feeding ecdysone (E) to larvae almost fully rescues the phenotypes.

(Yamanaka et al., 2015)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rab3^KK108633, Scer\GAL4^elav.PU has abnormal neuroanatomy | larval stage phenotype, enhanceable by Sec15^GD12109, Scer\GAL4^elav.PU

(Kang et al., 2024)

Rab3^KK108633, Scer\GAL4^elav.PU has abnormal neuroanatomy | larval stage phenotype, enhanceable by Sec15^KK101708, Scer\GAL4^elav.PU