FlyBase Allele Report: Dmel\mir-let7[UAS.cCa]

General Information

Symbol

Dmel\mir-let7^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Caygill

FlyBase ID

FBal0240491

Feature type

allele

Associated gene

Dmel\mir-let7

Associated Insertion(s)

Carried in Construct

P{UAS-mir-let7.C}

Also Known As

UAS-let-7

Key Links

Transgenic product class

wild_type

(Caygill and Johnston, 2008)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Caygill and Johnston, 2008)

Mutagen

in vitro construct

(Caygill and Johnston, 2008)

Progenitor genotype

Carried in construct

P{UAS-mir-let7.C}

Cytology

Description

UASt regulatory sequences drive expression of a 366bp fragment containing let-7.

(Caygill and Johnston, 2008)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

mir-let7

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates cancer

modeled by ph-d⁵⁰⁵, ph-p⁵⁰⁵

(Jiang et al., 2018)

ameliorates brain cancer

modeled by brat^KK113206

(Jiang et al., 2018)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

long lived | female | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Gendron and Pletcher, 2017)

short lived | female | RU486 conditional, with Scer\GAL4^{Tub.Switch.PU}

(Gendron and Pletcher, 2017)

short lived | male | RU486 conditional, with Scer\GAL4^{elav.Switch.PO}

(Gendron and Pletcher, 2017)

visible, with Scer\GAL4^vg.PM

(Caygill and Johnston, 2008)

Phenotype Manifest In

wing, with Scer\GAL4^vg.PM

(Caygill and Johnston, 2008)

wing disc, with Scer\GAL4^vg.PM

(Caygill and Johnston, 2008)

wing disc | somatic clone, with Scer\GAL4^Act.PU

(Caygill and Johnston, 2008)

Detailed Description

Statement

Reference

Expressing mir-let7^UAS.cCa under the control of Scer\GAL4^{Tub.Switch.PU} significantly reduces the adult life span of females treated with RU486 (to switch on transgene expression), with no effect on feeding duration or frequency compared to untreated and wild-type controls. Expression under the control of Scer\GAL4^{elav.Switch.PO} under RU486 treatment results in long lived females, but short lived males compared to untreated controls.

Expressing mir-let7^UAS.cCa under the control of either Scer\GAL4^Dh44.PU or Scer\GAL4^GMR57E10 has no effect on life span in females.

(Gendron and Pletcher, 2017)

Most clones of cells in the wing disc expressing let-7^Scer\UAS.cCa under the control of Scer\GAL4^Act stop dividing after 30 hours of clonal growth.

Expression of let-7^Scer\UAS.cCa under the control of Scer\GAL4^vg.PM results in a reduction in size of the third instar wing disc and of the adult wing.

(Caygill and Johnston, 2008)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

mir-let7^UAS.cCa is a suppressor of abnormal neuroanatomy phenotype of usp^GAL4::LBD.hs

(Kucherenko et al., 2012)

mir-let7^UAS.cCa is a suppressor of abnormal neuroanatomy phenotype of EcR^GAL4::LBD.hs

(Kucherenko et al., 2012)

Phenotype Manifest In

Suppressor of

Statement

Reference

mir-let7^UAS.cCa is a suppressor of lobe system of adult mushroom body phenotype of usp^GAL4::LBD.hs

(Kucherenko et al., 2012)

mir-let7^UAS.cCa is a suppressor of lobe system of adult mushroom body phenotype of EcR^GAL4::LBD.hs

(Kucherenko et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of let-7^Scer\UAS.cCa rescues the mushroom body α/β lobe defects seen in animals expressing usp^{hs.T:Scer\GAL4}.

Co-expression of let-7^Scer\UAS.cCa rescues the mushroom body α/β lobe defects seen in animals expressing EcR^{hs.T:Scer\GAL4}.

(Kucherenko et al., 2012)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Expression of let-7^Scer\UAS.cCa under the control of Scer\GAL4^let-7-C rescues the reduction in the volume of the mushroom body α/β lobes and the reduction in olfactory short-term memory seen in animals lacking let-7 function (Df(2L)let-7-C^KO1/Df(2L)let-7-C^GKI animals carrying P{W8,let-7-C^Δlet-7}).

(Kucherenko et al., 2012)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

let-7^Scer\UAS.cCa

let-7^UAS.cCa

mir-let7^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Caygill

Secondary FlyBase IDs

References (5)

Report Sections

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