FB2026_01 , released March 12, 2026
FB2026_01 , released March 12, 2026
Allele: Dmel\Lasp1
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General Information
Symbol
Dmel\Lasp1
Species
D. melanogaster
Name
FlyBase ID
FBal0241830
Feature type
allele
Associated gene
Dmel\Lasp
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

amorphic allele - molecular evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Lee et al., 2008)

amorphic allele - molecular evidence

(Lee et al., 2008)
Mutagen
FLPase
(Lee et al., 2008)
Progenitor genotype
PBac{RB}Laspe00056
(Lee et al., 2008)
PBac{RB}Laspe03191
(Lee et al., 2008)
Caused by aberration
Df(3L)Lasp1
Cytology
Description

Scer\FRT-mediated recombination between the two progenitor insertions has resulted in the deletion of the intervening sequence, which removes the second exon of Lasp and also removes intronic sequences, which include the nested CG9692 transcription unit.

(Lee et al., 2008)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
deletion
3L:16,682,455..16,709,311 [-]
Comment:

Deletion created by using piggyBac elements PBac{RB}Laspe00056 and PBac{RB}Laspe03191 for heat shock-mediated FLP/FRT recombination. Location of deletion on reference sequence inferred by FlyBase curator.

(Lee et al., 2008)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Indirect flight muscles from Lasp1 and Lasp1/Laspy41 mutants show no gross defects, but sarcomeres as well as thin filaments are 12% shorter than wild type. Myofibrils have an increased diameter, owing to both a 50% increase in the number of filaments and an increase in filament spacing.

      The flight ability of 3-5 day old flies is partially impaired and deteriorates in older flies.

      In 3-day old Lasp1 flies, I-band appearance in indirect flight muscles appears almost wild type, but in 18-day old flies, the I-band is wider than wild type. 18-day old mutant muscle also displays a thin filament packing/stability defect.

      (Fernandes and Schöck, 2014)

      Hub cells fail to localise to the apical tip of the testis in homozygous males. Germ cells are still present in 14 day old mutant males. Cysts coil prematurely and disintegrate into individual sperm at the coiling stage in the mutant males. Filamentous actin is only weakly visible at the assembly stage of actin cones in mutant spermatids. At the migrating stage, filamentous actin is barely visible or not visible at all. The mutant cysts show incomplete and slower spermatid individualisation compared to controls. The individualised spermatids are surrounded by a loose membrane and membrane fragments are found between spermatids.

      (Lee et al., 2008)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      Lasp[+]/Lasp1 is an enhancer of hub cell phenotype of mys8

      (Lee et al., 2008)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      mys8 ; Lasp1/+ males show a stronger hub cell mislocalisation phenotype than mys8 males. The double mutant males have wild-type fertility.

      (Lee et al., 2008)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Lasp1 is rescued by LaspL.UAS.Tag:FLAG,Tag:polyHis/Scer\GAL4Mef2.PR

      (Fernandes and Schöck, 2014)

      Lasp1 is rescued by Scer\GAL4Mef2.PR/LaspS.UAS.Tag:FLAG,Tag:polyHis

      (Fernandes and Schöck, 2014)

      Lasp1 is rescued by Scer\GAL4Mef2.PR/LaspL.UAS.GFP

      (Fernandes and Schöck, 2014)

      Lasp1 is rescued by Scer\GAL4Mef2.PR/LaspΔLIM.L.UAS.Tag:FLAG,Tag:polyHis

      (Fernandes and Schöck, 2014)
      Partially rescued by

      Lasp1 is partially rescued by LaspY86S.L.UAS.Tag:FLAG,Tag:polyHis/Scer\GAL4Mef2.PR

      (Fernandes and Schöck, 2014)

      Lasp1 is partially rescued by Scer\GAL4ptc-559.1/LaspUAS.EGFP

      (Lee et al., 2008)

      Lasp1 is partially rescued by Scer\GAL4VP16.nanos.UTR/LaspUAS.EGFP

      (Lee et al., 2008)
      Not rescued by

      Lasp1 is not rescued by Scer\GAL4Mef2.PR/LaspΔNEB.L.UAS.Tag:FLAG,Tag:polyHis

      (Fernandes and Schöck, 2014)

      Lasp1 is not rescued by Scer\GAL4Mef2.PR/LaspΔSH3.L.UAS.Tag:FLAG,Tag:polyHis

      (Fernandes and Schöck, 2014)

      Lasp1 is not rescued by LaspY121S.L.UAS.Tag:FLAG,Tag:polyHis/Scer\GAL4Mef2.PR

      (Fernandes and Schöck, 2014)
      Comments

      Scer\GAL4Mef2.PR-mediated expression of LaspL.Scer\UAS.T:Zzzz\FLAG,T:Zzzz\His6, LaspS.Scer\UAS.T:Zzzz\FLAG,T:Zzzz\His6 or LaspL.Scer\UAS.T:Avic\GFP rescues the sarcomere length and thin filament length phenotypes seen in Lasp1 indirect flight muscles.

      Scer\GAL4Mef2.PR-mediated expression of LaspL.Scer\UAS.T:Zzzz\FLAG,T:Zzzz\His6 rescues the reduced flight ability of Lasp1 flies.

      Scer\GAL4Mef2.PR-mediated expression of LaspΔLIM.L.Scer\UAS.T:Zzzz\FLAG,T:Zzzz\His6 rescues the sarcomere length, thin filament length, myofibril diameter and flight phenotypes seen in Lasp1 mutants.

      Scer\GAL4Mef2.PR-mediated expression of LaspY121S.L.Scer\UAS.T:Zzzz\FLAG,T:Zzzz\His6 cannot rescue the sarcomere length, thin filament length, myofibril diameter or flight phenotypes seen in Lasp1 mutants. EM analyses reveal that filament number, but not filament spacing, is partially rescued.

      Scer\GAL4Mef2.PR-mediated expression of LaspΔNEB.L.Scer\UAS.T:Zzzz\FLAG,T:Zzzz\His6 cannot rescue the sarcomere length, thin filament length, myofibril diameter or flight phenotypes seen in Lasp1 mutants.

      Scer\GAL4Mef2.PR-mediated expression of LaspΔSH3.L.Scer\UAS.T:Zzzz\FLAG,T:Zzzz\His6 cannot rescue the sarcomere length, thin filament length, myofibril diameter or flight phenotypes seen in Lasp1 mutants.

      Scer\GAL4Mef2.PR-mediated expression of LaspY86S.L.Scer\UAS.T:Zzzz\FLAG,T:Zzzz\His6 rescues the sarcomere length, thin filament length and flight phenotypes, but not the myofibril diameter phenotype seen in Lasp1 mutants. EM analyses reveal that filament spacing, but not filament number, is partially rescued.

      (Fernandes and Schöck, 2014)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      Lasp1
      (Fernandes and Schöck, 2014, Lee et al., 2008)
      Name Synonyms
      Secondary FlyBase IDs
        References (2)