FlyBase Allele Report: Dmel\Drak[del]

General Information

Symbol

Dmel\Drak^del

Species

D. melanogaster

Name

FlyBase ID

FBal0246418

Feature type

allele

Associated gene

Dmel\Drak

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

FLPase

(Neubueser and Hipfner, 2010)

recombination

(Neubueser and Hipfner, 2010)

Progenitor genotype

PBac{RB}Drak^e03054

(Neubueser and Hipfner, 2010)

PBac{WH}f07790

(Neubueser and Hipfner, 2010)

Cytology

Description

Recombination between the two progenitor insertions has resulted in deletion of the genomic sequence between them, which removes almost the entire Drak gene without affecting neighbouring genes.

(Neubueser and Hipfner, 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:11,493,157..11,554,811 [+]

Comment:

Deletion of Drak resulting from FLP-catalyzed recombination using the FRT-containing transposons PBac{WH}f07790 and PBac{RB}Drak^e03054.

(Neubueser and Hipfner, 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates high grade glioma

modeled by Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, btl::Egfr^λ.UAS

(Chen et al., 2019)

modeled by btl::Egfr^λ.UAS

(Chen et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

fertile

(Neubueser and Hipfner, 2010)

viable

(Chen et al., 2019, Neubueser and Hipfner, 2010)

viable (with Drak^KO)

(Neubueser and Hipfner, 2010)

visible

(Neubueser and Hipfner, 2010)

Phenotype Manifest In

metathoracic leg

(Neubueser and Hipfner, 2010)

wing

(Neubueser and Hipfner, 2010)

Detailed Description

Statement

Reference

The wing area of homozygous flies is significantly smaller than that of controls, with the wings being specifically shortened in the proximal-distal axis. The mutant flies sometimes show shortening of the legs, usually restricted to the T3 leg; leg segments (primarily the femur and tibia) are shortened and abnormally bent.

Drak^del/Drak^KO adults show a reduction in wing area compared to controls and a partially penetrant phenotype of T3 leg defects.

(Neubueser and Hipfner, 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Drak^del has visible phenotype, enhanceable by Rho1^72O/Rho1[+]

(Neubueser and Hipfner, 2010)

Drak^del has visible phenotype, enhanceable by zip²/zip[+]

(Neubueser and Hipfner, 2010)

Drak^del has visible phenotype, enhanceable by Sb^63b/Sb[+]

(Neubueser and Hipfner, 2010)

Suppressed by

Statement

Reference

Drak^del/Drak^KO, rok[+]/Rok² has lethal phenotype, suppressible by flw^G0172/flw^G0172

(Neubueser and Hipfner, 2010)

Drak^del/Drak^KO, rok[+]/Rok² has lethal phenotype, suppressible by sqh^E20.E21

(Neubueser and Hipfner, 2010)

NOT suppressed by

Statement

Reference

Drak^del/Drak^KO, rok[+]/Rok² has lethal phenotype, non-suppressible by sqh^{A20.A21.Tag:FLAG}

(Neubueser and Hipfner, 2010)

Suppressor of

Statement

Reference

Drak^del/Drak^del is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Drak^del/Drak^del is a suppressor of neoplasia | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Drak^del/Drak^del is a suppressor of abnormal neuroanatomy | third instar larval stage phenotype of Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Drak^del/Drak^del is a suppressor of hyperplasia | third instar larval stage phenotype of Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Other

Statement

Reference

Drak^del, rok[+]/Rok² has lethal phenotype

(Neubueser and Hipfner, 2010)

Drak^del, Rok² has lethal | embryonic stage phenotype

(Neubueser and Hipfner, 2010)

Drak^del/Drak^KO, rok[+]/Rok² has lethal phenotype

(Neubueser and Hipfner, 2010)

Drak^del/Drak^KO, rok[+]/Rok² has increased cell death phenotype

(Neubueser and Hipfner, 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Drak^del has wing phenotype, enhanceable by Rho1^72O/Rho1[+]

(Neubueser and Hipfner, 2010)

Drak^del has wing phenotype, enhanceable by zip²/zip[+]

(Neubueser and Hipfner, 2010)

Drak^del has wing phenotype, enhanceable by Sb^63b/Sb[+]

(Neubueser and Hipfner, 2010)

Suppressed by

Statement

Reference

Drak^del has metathoracic leg phenotype, suppressible by flw^G0172

(Neubueser and Hipfner, 2010)

Suppressor of

Statement

Reference

Drak^del/Drak^del is a suppressor of larval brain neuropil glial cell | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Drak^del/Drak^del is a suppressor of larval brain | third instar larval stage phenotype of Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Drak^del/Drak^del is a suppressor of larval brain neuropil glial cell | third instar larval stage phenotype of Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Drak^del/Drak^del is a suppressor of larval brain | third instar larval stage phenotype of Pi3K92E^{UAS.Tag:MYC,Tag:PM(hKRAS)}, Scer\GAL4^repo.PU, btl::Egfr^λ.UAS

(Chen et al., 2019)

Drak^del is a suppressor of wing phenotype of flw^G0172

(Neubueser and Hipfner, 2010)

Other

Statement

Reference

Drak^del, rok[+]/Rok² has larval tracheal system phenotype

(Neubueser and Hipfner, 2010)

Drak^del, Rok² has cephalopharyngeal skeleton phenotype

(Neubueser and Hipfner, 2010)

Drak^del, Rok² has embryonic head phenotype

(Neubueser and Hipfner, 2010)

Drak^del/Drak^KO, rok[+]/Rok² has wing disc phenotype

(Neubueser and Hipfner, 2010)

Drak^del/Drak^KO, rok[+]/Rok² has wing pouch phenotype

(Neubueser and Hipfner, 2010)

Additional Comments

Genetic Interactions

Statement

Reference

The shortening of the wing which is seen in Drak^del flies is enhanced if the flies are also heterozygous for one of Rho1^72O, zip² or Sb^63b.

More than half of Drak^del rok²/Drak^del third instar larvae are missing random elements of the tracheal tree.

The wing discs of Drak^del rok²/Drak^KO third instar larvae are composed of unusually thin epithelial layers which are grossly distorted. The wing pouch region has folds which project basally out of the plane of the epithelium, and these distortions are accompanied by massive apoptosis.

100% of Drak^del rok² double homozygous embryos die before hatching. They show a fully penetrant head defect, which is characterized by an anterior hole in the embryonic cuticle and anterior malformations of the cephalopharyngeal skeleton.

The wing defects of flw^G0172 escapers are suppressed by Drak^del. The T3 leg defects seen in Drak^del mutants are suppressed by flw^G0172.

(Neubueser and Hipfner, 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Drak^Del

(Robertson et al., 2012)

Drak^del

(Chen et al., 2019)

Drak^null

(Chen et al., 2019)

drak^del

(Deichsel et al., 2024, Deichsel et al., 2024, Neubueser and Hipfner, 2010)

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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