FlyBase Allele Report: Dmel\Lsd-1[1]

General Information

Symbol

Dmel\Lsd-1¹

Species

D. melanogaster

Name

FlyBase ID

FBal0247947

Feature type

allele

Associated gene

Dmel\Lsd-1

Associated Insertion(s)

P{?EP}Lsd-1¹

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Beller et al., 2010, Beller et al., 2010)

Progenitor genotype

P{EP}Lsd-1^G4304

(Beller et al., 2010, Beller et al., 2010)

Associated Insertion(s)

P{?EP}Lsd-1¹

Cytology

Description

Imprecise excision of the progenitor insertion, resulting in partial deletion of nucleotides -509 to +82 relative to the putative Lsd-1 start site (this deletes sequences encoding amino acid residues 1-28). 2.2kb of the original inserted element remains.

(Beller et al., 2010)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

delins

3R:23,763,804..23,764,465 [+]

Comment:

Estimated boundaries of a deletion resulting from the imprecise excsion of P{EP}Lsd-1^G4304, which removes sequences from -509 relative to the Lsd-1 translation start site up through amino acid 28. 2.2 kb of residual P element sequences remain.

(Beller et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of obesity

CEA

(Beller et al., 2010)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of obesity

is exacerbated by AkhR¹

(Beller et al., 2010)

is exacerbated by bmm¹

(Beller et al., 2010)

is ameliorated by Pka-C1^UAS.cKa

(Beller et al., 2010)

is ameliorated by bmm^UAS.cGa

(Beller et al., 2010)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal stress response

(Beller et al., 2010)

abnormal stress response (with Lsd-1²)

(Beller et al., 2010)

increased body size

(Beller et al., 2010)

Phenotype Manifest In

lipid droplet

(Beller et al., 2010)

Detailed Description

Statement

Reference

Homozygotes develop into obese but otherwise normal-looking adults. The obesity phenotype progressively manifests, starting at day 2 of adult life. The obesity phenotype is nutrition dependent; on a high-sugar diet, the cumulative food intake of the mutant flies during the first 6 days of adult life is approximately one-third higher than that of controls and their body fat content is doubled. On a low-sugar diet, both mutant and wild-type controls show a similar food intake, but the mutant flies accumulate up to 50% more body fat than the controls. Activity patterns and total cumulative locomotor activity of mutant and control flies are comparable during obesity development.

Fat body cells of mutant animals have a giant lipid droplet phenotype typical for unilocular fat cells. This phenotype is cell autonomous. The phenotype develops during larval stages, prior to the onset of obesity.

Homozygous, Lsd-1¹/Lsd-1² and Lsd-1¹/Df(3R)mbc-30 flies have double the fat content of control flies.

Homozygous and Lsd-1¹/Lsd-1² flies show increased resistance to starvation compared to control flies. Homozygous flies show incomplete storage fat mobilisation under starvation conditions.

(Beller et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Lsd-1¹ has increased body size phenotype, non-suppressible by Scer\GAL4^fat/Hsap\PLIN1^UAS.cBa

(Beller et al., 2010)

Other

Statement

Reference

Lsd-1¹, bmm¹ has abnormal stress response phenotype

(Beller et al., 2010)

AkhR¹, Lsd-1¹ has abnormal stress response phenotype

(Beller et al., 2010)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Lsd-1¹ has lipid droplet phenotype, non-suppressible by Scer\GAL4^fat/Hsap\PLIN1^UAS.cBa

(Beller et al., 2010)

Other

Statement

Reference

Lsd-1¹, Lsd-2⁵¹ has lipid droplet phenotype

(Beller et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Lsd-1¹ bmm¹ double mutant flies are more obese than either single mutant and the double mutants fail to mobilise their fat storage under starvation conditions are hypersensitive to starvation.

Lsd-1¹ GRHR¹ double mutant flies are more obese than either single mutant. The double mutants show resistance to starvation compared to controls.

Expression of bmm^Scer\UAS.cGa under the control of Scer\GAL4^fat suppresses the increase in fat content which is seen in Lsd-1¹ flies and the double mutant flies are leaner than flies expressing bmm^Scer\UAS.cGa under the control of Scer\GAL4^fat in a Lsd-1[+] background.

Expression of Pka-C1^Scer\UAS.cKa under the control of Scer\GAL4^fat suppresses the increased fat content seen in Lsd-1¹ flies.

Lsd-1¹ Lsd-2⁵¹ double mutant adults have strongly reduced body fat stores at hatching (in contrast to the normal levels seen in newly hatched single mutants). The double mutants recover to have levels of stored body fat which are similar to those seen in the lean Lsd-2⁵¹ single mutants within the first 6 days after eclosion. The fat body cells contain large lipid droplets. During aging, the fat content of the double mutants is adjusted to a level between the level seen in each single mutant. Under a starvation/re-feeding scheme, the double mutant flies show attenuated lipid metabolism and re-accumulation compared to controls flies.

(Beller et al., 2010)

Xenogenetic Interactions

Statement

Reference

The giant lipid droplet and obesity phenotypes seen in Lsd-1¹ mutants are not rescued by expression of Hsap\PLIN1^Scer\UAS.cBa under the control of Scer\GAL4^fat.

(Beller et al., 2010)

Complementation and Rescue Data

Rescued by

Lsd-1¹ is rescued by Lsd-1^+t3.5

(Beller et al., 2010)

Lsd-1¹ is rescued by Scer\GAL4^fat/Lsd-1^UAS.cBa

(Beller et al., 2010)

Comments

Lsd-1^+t3.5 rescues the increased body fat content of Lsd-1¹ flies.

Expression of Lsd-1^Scer\UAS.cBa under the control of Scer\GAL4^fat rescues the increased starvation resistance seen in Lsd-1¹ flies. Lipid droplet size is reduced in these animals compared to Lsd-1¹ single mutants.

One copy of Lsd-1^+t3.5 partially rescues the increased starvation resistance seen in Lsd-1¹ flies.

(Beller et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Lsd-1¹

plin¹

(Beller et al., 2010, Beller et al., 2010)

Name Synonyms

Secondary FlyBase IDs

References (2)

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