FlyBase Allele Report: Scer\GAL4[GMR82E12]

General Information

Symbol

Scer\GAL4^GMR82E12

Species

S. cerevisiae

Name

FlyBase ID

FBal0255338

Feature type

allele

Associated gene

Scer\GAL4

Associated Insertion(s)

Carried in Construct

P{GMR82E12-GAL4}

Also Known As

GMR82E12-GAL4, 82E12-Gal4, A08n-Gal4, R82E12

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Pfeiffer et al., 2011.2.9)

Progenitor genotype

Carried in construct

P{GMR82E12-GAL4}

Cytology

Description

A 3191 base pair fragment from the flanking non-coding or intronic region of CG4575 is fused upstream of a Drosophila core synthetic promoter (DSCP) that is followed by sequence encoding a GAL4 driver.

(Pfeiffer et al., 2011.2.9)

Allele components

Component

Use(s)

Regulatory region(s)

GMR82E12

Encoded product / tool

GAL4

binary expression system - driver

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter (Gal4 UAS)

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

larval A08n neuron

(Hu et al., 2017)

adult stage

antennal mechanosensory and motor center | faint | restricted

(Jenett and Svirskas, 2012.9.24)

Additional Information

Statement

Reference

Marker for

larval A08n neuron

(Hu et al., 2017)

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal larval locomotory behavior | light conditional | chemical conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.13XLexAop2.Venus}, Ecol\lexA^{VP16.cytoFLARE1.0.UAS}, TEV\NIa^{S153N.UAS.CaM}

(Zhou et al., 2025)

abnormal larval locomotory behavior | light conditional | chemical conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Zhou et al., 2025)

abnormal larval locomotory behavior | light conditional | chemical conditional, with Ecol\lexA^{VP16.cytoFLARE1.0.UAS}, Gthe\ACR1^{lexAop.d.EYFP}, TEV\NIa^{S153N.UAS.CaM}

(Zhou et al., 2025)

abnormal larval locomotory behavior | light conditional | chemical conditional, with Gthe\ACR1^UAS.d.EYFP

(Zhou et al., 2025)

abnormal locomotor behavior | larval stage | chemical conditional, with Ctet\tetX^TNT-LC.UAS

(Lopez-Bellido et al., 2019)

abnormal locomotor behavior | larval stage | conditional, with Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}

(Hu et al., 2017)

abnormal locomotor behavior | larval stage | conditional, with Crei\ChR2^UAS.cSa

(Vogelstein et al., 2014)

abnormal neuroanatomy | larval stage, with Cbeta\DT^A-I.UAS

(Kaneko et al., 2024)

abnormal neuroanatomy | larval stage, with dpp^HMS00011

(Kaneko et al., 2024)

abnormal neuroanatomy | larval stage, with dpp^JF02794

(Kaneko et al., 2024)

abnormal pain response | larval stage, with Hsap\KCNJ2^UAS.EGFP

(Hu et al., 2017)

Phenotype Manifest In

axon terminus | larval stage, with Cbeta\DT^A-I.UAS

(Kaneko et al., 2024)

axon terminus | larval stage, with dpp^HMS00011

(Kaneko et al., 2024)

axon terminus | larval stage, with dpp^JF02794

(Kaneko et al., 2024)

larval nociceptive neuropil, with Cbeta\DT^A-I.UAS

(Kaneko et al., 2024)

larval nociceptive neuropil, with dpp^HMS00011

(Kaneko et al., 2024)

larval nociceptive neuropil, with dpp^JF02794

(Kaneko et al., 2024)

Detailed Description

Statement

Reference

Inhibition (using Ctet\tetX^TNT-LC.UAS) of Scer\GAL4^GMR82E12 cells significantly reduces larval escape behavior in response to HCl application, compared to controls.

(Lopez-Bellido et al., 2019)

Hyperpolarization (using Hsap\KCNJ2^UAS.EGFP) of Scer\GAL4^GMR82E12 cells in larvae significantly reduces nociceptive movement responses following stimulation with a von Frey filament, compared to controls; there is no effect on the response to gentle touch or locomotion speed, compared to controls.

Optogenetic activation (using Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus} with 625 nm (red) light) of Scer\GAL4^GMR82E12 cells in larvae significantly increases rolling responses in the absence of pain stimuli, compared to controls.

(Hu et al., 2017)

Optogenetic activation (using Crei\ChR2^UAS.cSa with 470 nm (blue) light) of Scer\GAL4^GMR82E12 cells in larvae results in a significant increase in escape behaviors, compared to controls.

(Vogelstein et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

Cnoc\ChR1::Csub\ChR^{CsChrimson.IVS.20xUAS.Venus}, Scer\GAL4^GMR82E12 has abnormal locomotor behavior | larval stage | conditional phenotype, non-enhanceable by Ecol\lexA^GMR27H06/Hsap\KCNJ2^lexAop.cHa

(Hu et al., 2017)