FlyBase Allele Report: Dmel\rpr[UTR.UAS.Tag:HA]

General Information

Symbol

Dmel\rpr^{UTR.UAS.Tag:HA}

Species

D. melanogaster

Name

FlyBase ID

FBal0260662

Feature type

allele

Associated gene

Dmel\rpr

Associated Insertion(s)

Carried in Construct

P{UAS-rpr-HA}

Key Links

Transgenic product class

wild_type

(Sandu et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Sandu et al., 2010)

Mutagen

in vitro construct

(Sandu et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-rpr-HA}

Cytology

Description

UAS regulatory sequences drive expression of rpr coding sequences flanked by rpr 5' and 3' UTRs and tagged at the C-terminus with Tag:HA.

(Sandu et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

rpr

Tagged with

Tag:HA

epitope tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

increased cell death, with Scer\GAL4^GMR.PF

(Sandu et al., 2010)

Phenotype Manifest In

eye, with Scer\GAL4^GMR.PF

(Sandu et al., 2010)

wing, with Scer\GAL4^sd.PU

(Fereres et al., 2013)

Detailed Description

Statement

Reference

Scer\GAL4^sd.PU-mediated expression of rpr^{UTR.Scer\UAS.T:Ivir\HA1} produces flies with no wings.

(Fereres et al., 2013)

Expression of rpr^{UTR.Scer\UAS.T:Ivir\HA1} in the developing eye under the control of Scer\GAL4^GMR.PF produces severe eye ablation.

(Sandu et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^GMR.PF, rpr^{UTR.UAS.Tag:HA} has increased cell death phenotype, suppressible by Diap1^6-3s/th[+]

(Sandu et al., 2010)

Scer\GAL4^GMR.PF, rpr^{UTR.UAS.Tag:HA} has increased cell death phenotype, suppressible by Diap1^23-4s/th[+]

(Sandu et al., 2010)

Scer\GAL4^GMR.PF, rpr^{UTR.UAS.Tag:HA} has increased cell death phenotype, suppressible by hid^GD1673/Scer\GAL4^GMR.PF

(Sandu et al., 2010)

Scer\GAL4^GMR.PF, rpr^{UTR.UAS.Tag:HA} has increased cell death phenotype, suppressible by hid^A206/W[+]

(Sandu et al., 2010)

Scer\GAL4^GMR.PF, rpr^{UTR.UAS.Tag:HA} has increased cell death phenotype, suppressible by hid^rvX1/W[+]

(Sandu et al., 2010)

Suppressor of

Statement

Reference

rpr^{UTR.UAS.Tag:HA}/Scer\GAL4^GMR.PF is a suppressor of increased cell death phenotype of hid^GD1673

(Sandu et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^sd.PU, rpr^{UTR.UAS.Tag:HA} has wing phenotype, suppressible | partially by Scer\GAL4^sd.PU/SkpA^UAS.OB

(Fereres et al., 2013)

Scer\GAL4^GMR.PF, rpr^{UTR.UAS.Tag:HA} has eye phenotype, suppressible by Diap1^6-3s/th[+]

(Sandu et al., 2010)

Scer\GAL4^GMR.PF, rpr^{UTR.UAS.Tag:HA} has eye phenotype, suppressible by Diap1^23-4s/th[+]

(Sandu et al., 2010)

Scer\GAL4^GMR.PF, rpr^{UTR.UAS.Tag:HA} has eye phenotype, suppressible by hid^GD1673/Scer\GAL4^GMR.PF

(Sandu et al., 2010)

Scer\GAL4^GMR.PF, rpr^{UTR.UAS.Tag:HA} has eye phenotype, suppressible by hid^A206/W[+]

(Sandu et al., 2010)

Scer\GAL4^GMR.PF, rpr^{UTR.UAS.Tag:HA} has eye phenotype, suppressible by hid^rvX1/W[+]

(Sandu et al., 2010)

Suppressor of

Statement

Reference

rpr^{UTR.UAS.Tag:HA}/Scer\GAL4^GMR.PF is a suppressor of eye phenotype of hid^GD1673

(Sandu et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of SkpA^Scer\UAS.OB partially restores wings to flies expressing rpr^{UTR.Scer\UAS.T:Ivir\HA1} via Scer\GAL4^sd.PU.

(Fereres et al., 2013)

A th^6-3s or th^23-4s heterozygous background significantly suppresses apoptosis induced by expression of rpr^{UTR.Scer\UAS.T:Ivir\HA1} under the control of Scer\GAL4^GMR.PF.

RNAi-mediated knockdown of W through expression of W^GD1673 under the control of Scer\GAL4^GMR.PF considerably suppressed the cell death phenotype of flies expressing rpr^{UTR.Scer\UAS.T:Ivir\HA1} in the developing eye.

A W^hid-A206 or W^rvX1 heterozygous background suppresses the degree of rpr^{UTR.Scer\UAS.T:Ivir\HA1}-indued eye ablation.

(Sandu et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

rpr^{UTR.UAS.Tag:HA} is rescued by rpr^GD4698/Scer\GAL4^GMR.PF

(Sandu et al., 2010)

Rescues

rpr^{UTR.UAS.Tag:HA}/Scer\GAL4^GMR.PF rescues rpr^LZ.UAS

(Sandu et al., 2010)

Comments

Co-expression of rpr^{UTR.Scer\UAS.T:Ivir\HA1} rescues eye morphology in flies also expressing rpr^{Scer\UAS.T:Scer\GCN4} under the control of Scer\GAL4^GMR.PF.

Co-expression of rpr^GD4698 rescues eye morphology defects caused by expression of rpr^{UTR.Scer\UAS.T:Ivir\HA1} (where both transgenes are under the control of Scer\GAL4^GMR.PF).

(Sandu et al., 2010)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

rpr^{UTR.Scer\UAS.T:Ivir\HA1}

rpr^{UTR.UAS.Tag:HA}

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

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