FlyBase Allele Report: Dmel\Atg6[HMS01483]

General Information

Symbol

Dmel\Atg6^HMS01483

Species

D. melanogaster

Name

FlyBase ID

FBal0263391

Feature type

allele

Associated gene

Dmel\Atg6

Associated Insertion(s)

Carried in Construct

P{TRiP.HMS01483}

Also Known As

Atg6 RNAi, atg6^shR, UAS-Atg6^RNAi, UAS-Atg6 RNAi

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Ni et al., 2011.7.19)

Mutagen

in vitro construct

(Ni et al., 2011.7.19)

Progenitor genotype

Carried in construct

P{TRiP.HMS01483}

Cytology

Description

UAS regulatory sequences drive expression of a short inverted repeat.

(Ni et al., 2011.7.19)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

dsRNA-HMS01483

Also carries

loxP

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates C9orf72 frontotemporal dementia and/or amyotrophic lateral sclerosis

modeled by Zzzz\GGGGCC^30.UAS.EGFP

(Cunningham et al., 2020)

ameliorates cancer

modeled by Raf^F22.hs

(Zhao et al., 2021)

exacerbates Alzheimer's disease 1

modeled by Hsap\APP^2xAPP.SP.UAS

(Jang et al., 2024)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | P-stage, with Scer\GAL4^NP0001

(Xu et al., 2015)

increased occurrence of cell division | adult stage, with Scer\GAL4^esg.PU

(Zhang et al., 2019)

Phenotype Manifest In

Detailed Description

Statement

Reference

Adulthood-only expression of Atg6^HMS01483 under the control of Scer\GAL4^esg.PU leads to a significant increase in the number of mitotic cells (phospho-H3 staining) in the adult midgut, as compared to controls. (The temporal regulation of expression is dependent on Gal80[ts] and induced by temperature shift to 29[o]C for 5-6 days during adulthood).

(Zhang et al., 2019)

The expression of Atg6^HMS01483 under the control of Scer\GAL4^Cg.PA blocks the starvation-induced accumulation of acidic vesicles (LysoTracker-positive) in the early third instar larval fat body, as compared to controls. Under standard conditions, there is no change in the number of autophagosome-like structures (Atg8a-positive), as compared to controls.

(Liu et al., 2018)

Expression of Atg6^HMS01483 under the control of Scer\GAL4^{Myo31DF-NP0001} results in delayed midgut removal after pupariation, with increased size of the gastric caeca 4 hrs after puparium formation, but no difference in autophagy levels 2 hrs after puparium formation, as compared to controls.

(Xu et al., 2015)

Embryos derived from females expressing Atg6^HMS01483 under the simultaneous control of Scer\GAL4^{otu.T:Hsim\VP16}, Scer\GAL4^nos.PG and Scer\GAL4^{nos.UTR.T:Hsim\VP16} show a significant delay in the clearance of the paternal mitochondrial derivatives compared to wild type.

(Politi et al., 2014)

Expression of Atg6^HMS01483 under the control of Scer\GAL4^Mef2.PR is able to significantly suppress the formation of autophagosomes in the muscles of chloroquine-treated larvae starved on low-nutrient food for 6 hours.

(Zirin et al., 2013)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Suppressor of

Statement

Reference

Atg6^HMS01483, Scer\GAL4^Cg.PA is a non-suppressor of decreased cell size | third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Pink1^UAS.cCa/Atg6^HMS01483, Scer\GAL4^Cg.PA is a non-suppressor of decreased cell size | third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Phenotype Manifest In

Suppressor of

Statement

Reference

Atg6^HMS01483, Scer\GAL4^Cg.PA is a suppressor of autophagosome | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Atg6^HMS01483, Scer\GAL4^Cg.PA is a suppressor | partially of fat cell | early third instar larval stage phenotype of Scer\GAL4^Cg.PA, Tom40^JF02030

(Liu et al., 2018)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

35741

y¹ sc^* v¹ sev²¹; P{TRiP.HMS01483}attP2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Atg6^HMS01483

(Billes et al., 2018)

atg6^RNAi

(Zhao et al., 2021)

atg6^Ri

(Tarayrah-Ibraheim et al., 2021)

atg6^shR

(Politi et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

Open Close